{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T14:48:42Z","timestamp":1784731722073,"version":"3.55.0"},"reference-count":41,"publisher":"Walter de Gruyter GmbH","issue":"3","license":[{"start":{"date-parts":[[2024,9,1]],"date-time":"2024-09-01T00:00:00Z","timestamp":1725148800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Infections with hookworms (\n                    <jats:italic>Necator americanus<\/jats:italic>\n                    and\n                    <jats:italic>Ancylostoma duodenale<\/jats:italic>\n                    ) remain a major public health problem in low- and middle-income countries. However, the information about the distribution of each species is inaccurate in many countries since their traditional diagnosis is based only on the identification of eggs in stool under a microscope. We aimed to identify the prevalence of hookworm species using morphological stools to identify L3 larvae to gain insights into the distribution of both species in five regions of Cameroon. Samples were collected from schoolchildren in five regions and 34 subdivisions of Cameroon and examined using the Kato-Katz method. We randomly selected a total of 157 samples among hookworm's positive stool samples. They were cultured using the Harada-Mori test-tube technique. The morphological identification of a total of 8063 isolated hookworm filariform larvae L3 was conducted following established criteria. The sensitivity rate to the Harada-Mori technique was 58 %. Among the 8063 L3 larvae identified during this study, 230 (2.95 %) of L3 larvae were identified as\n                    <jats:italic>A. duodenale<\/jats:italic>\n                    , and 7833 (97.15 %) of L3 larvae were identified as\n                    <jats:italic>N. americanus<\/jats:italic>\n                    .\n                    <jats:italic>A. duodenale<\/jats:italic>\n                    was observed only in the Mouanko subdivision in the Littoral region. The complementary use of the Kato Katz and the Harada-Mori culture techniques to screen hookworm infections contributes to the differentiation of\n                    <jats:italic>N. americanus<\/jats:italic>\n                    and\n                    <jats:italic>A. duodenale<\/jats:italic>\n                    as the two hookworm species in Cameroon. An extended molecular study in the localities where only\n                    <jats:italic>N. americanus<\/jats:italic>\n                    has been identified is necessary to reach more conclusions on the distribution of hookworm species in Cameroon.\n                  <\/jats:p>","DOI":"10.2478\/helm-2024-0025","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T16:28:37Z","timestamp":1734020917000},"page":"224-231","source":"Crossref","is-referenced-by-count":3,"title":["Morphological identification of hookworm species in five regions of Cameroon"],"prefix":"10.2478","volume":"61","author":[{"given":"C.N.","family":"Dongmo","sequence":"first","affiliation":[{"name":"Laboratory of Parasitology and Ecology , Department of Animal Biology and Physiology , Faculty of Science, University of Yaounde I , P.O. Box 812 , Yaounde , Cameroon"},{"name":"Centre for Schistosomiasis and Parasitology , P.O. Box 7244 , Yaound\u00e9 , Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.B.","family":"Jatsa","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Physiology , Department of Animal Biology and Physiology , Faculty of Science, University of Yaound\u00e9 I , P.O. Box 812 , Yaound\u00e9 , Cameroon"},{"name":"Centre for Schistosomiasis and Parasitology , P.O. Box 7244 , Yaound\u00e9 , Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L-A","family":"Tchuem Tchuente","sequence":"additional","affiliation":[{"name":"Laboratory of Parasitology and Ecology , Department of Animal Biology and Physiology , Faculty of Science, University of Yaounde I , P.O. Box 812 , Yaounde , Cameroon"},{"name":"Centre for Schistosomiasis and Parasitology , P.O. Box 7244 , Yaound\u00e9 , Cameroon"},{"name":"National Program for the Control of Schistosomiasis and Intestinal Helminthiasis, Ministry of Public Health , Yaounde , Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2024,12,12]]},"reference":[{"key":"2026060619564311128_j_helm-2024-0025_ref_001","doi-asserted-by":"crossref","unstructured":"Adenusi, A.A. (1997): The distribution of Necator americanus and Ancylostoma duodenale among schoolchildren in Lagos, Nigeria. Trans R Soc Trop Med Hyg, 91: 270. DOI: 10.1016\/s0035-9203(97)90069-9","DOI":"10.1016\/S0035-9203(97)90069-9"},{"key":"2026060619564311128_j_helm-2024-0025_ref_002","doi-asserted-by":"crossref","unstructured":"Adenusi A., Ogunyomi, E. (2003): Relative prevalence of the human hookworm species, Necator americanus and Ancylostoma duodenale in an urban community in Ogun State, Nigeria. Afr J Biotechnol, 2: 470 \u2013 473. 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