{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T11:03:30Z","timestamp":1779879810722,"version":"3.53.1"},"reference-count":15,"publisher":"Walter de Gruyter GmbH","issue":"3","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Corynosoma pseudohamanni<\/jats:italic>\n                    Zdzitowiecki, 1984 (Polymorphidae) was described from the intestinal tract of 5 species of seals, including the type and primary host, the Weddell seal\n                    <jats:italic>Leptonycotes weddellii<\/jats:italic>\n                    (Lesson) in the South Shetlands, West Antarctica.\n                    <jats:italic>Notothenia coriiceps<\/jats:italic>\n                    Richardson was the primary paratenic host of 14 fish hosts reported in the original description. We redescribed excysted juveniles from the body cavity of\n                    <jats:italic>N. coriiceps<\/jats:italic>\n                    collected off Galindez Island, Argentine Islands, and included its molecular analysis, SEM images, and Energy Dispersive X-ray Analysis (EDXA) for the first time. The identity and distribution of mineral elements in the center and edge of anterior, middle, and posterior proboscis hooks establish their taxonomic relevance. Samples were dehydrated through an ascending ethanol series and then critical point dried, mounted on stubs and coated with carbon with a thickness of 20 nm. The specimens were examined and positioned using the LYRA3 FIB-SEM (TESCAN, Brno \u2013 Kohoutovice, Czech Republic), equipped with a Phoenix energy-dispersive X-ray analyzer (Oxford Instruments, Abingdon, England). X-ray spot and live scan analyses were performed at 15 kV with a spot size 2. The AZtec version 4.3 software system (Oxford Instruments, Abingdon, England) was used. We demonstrated the highest calcium levels in all hooks and hook roots compared to sulfur and phosphorus. Here, for the first time, we report a new aspect of the elemental analysis of hooks, demonstrating the differential distribution of sulfur, phosphorous, calcium, and magnesium at the center and margins of anterior, middle, and posterior hooks and hook roots. Calcium was the most prevalent element in roots (root edge and root middle) and the center of middle of all hooks. Phosphorous was equally common but not as prevalent and was highest in the middle of the roots and the middle of all hooks. Sulfur was negligible or absent in the roots and middle of all hooks but highest at hook tips and the edge of the middle hook. The biological and taxonomic importance of this EDXA pattern is discussed, as well as its relevance to hook strength and flexibility, species identity, and comparative systematics. Comparative results were also presented for cystacanths of five other species of acanthocephalans for which EDXA patterns have been studied.\n                  <\/jats:p>","DOI":"10.2478\/helm-2025-0025","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T14:04:24Z","timestamp":1764165864000},"page":"218-229","source":"Crossref","is-referenced-by-count":0,"title":["Differential distribution of minerals in the proboscis hooks of\n                    <i>Corynosoma pseudohamanni<\/i>\n                    Zdzitowiecki, 1984 (Acanthocephala: Polymorphidae) juveniles from\n                    <i>Notothenia coriiceps<\/i>\n                    Richardson off Argentine Islands, West Antarctica"],"prefix":"10.2478","volume":"62","author":[{"given":"M. E.","family":"Caracciolo","sequence":"first","affiliation":[{"name":"Multiuser Center for Biomedical Phenomena Analysis (CMABio), State University of Amazonas (UEA) , Manaus , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O. M.","family":"Amin","sequence":"additional","affiliation":[{"name":"Institute of Parasitic Diseases , 11445 E. Via Linda, # 2-419 , Scottsdale , , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Wendt","sequence":"additional","affiliation":[{"name":"lnstitute of Biomedical Sciences, Federal University of Rio de Janeiro , Rio de Janeiro , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. YU.","family":"Rubtsova","sequence":"additional","affiliation":[{"name":"Institute of Parasitic Diseases , 11445 E. Via Linda, # 2-419 , Scottsdale , , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W De","family":"Souza","sequence":"additional","affiliation":[{"name":"Hertha Meyer Laboratory of Cellular Ultrastructure, Carlos Chagas Filho Institute of Biophysics, Center for Health Sciences, Federal University of Rio de Janeiro , Rio de Janeiro , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2025,11,26]]},"reference":[{"key":"2026052710154625430_j_helm-2025-0025_ref_001","doi-asserted-by":"crossref","unstructured":"Amin, O.M., Chaudhary, A., Singh, H.S. (2022): Morphological and molecular description of immature Southwellina hispida (Van Cleave, 1925) Witenberg, 1932 (Acanthocephala: Polymorphidae) from the body cavity of the paratenic host Gillichthys mirabilis Cooper (Gobiidae) in California, with analyses of the chemical composition of hooks and spines. Acta Parasitol, 67: 1107 \u2013 1125. DOI: 10.1007\/s11686-022-00552-2","DOI":"10.1007\/s11686-022-00552-2"},{"key":"2026052710154625430_j_helm-2025-0025_ref_002","doi-asserted-by":"crossref","unstructured":"Amin, O.M., Chaudhary, A., Caracciolo, M.E., Rubtsova, N.Yu., Wendt, C., Lisitsyna, O., Kuzmina, T.A., de Souza, W., Singh, H.S. (2025a): Redescription and molecular analysis of Corynosoma pseudohamanni Zdzitowiecki, 1984 (Acanthocephala: Polymorphidae) juveniles from Notothenia coriiceps Richardson in the water area of Argentine Islands, West Antarctica. Syst Parasitol, 102(1): 16. DOI: 10.1007\/s11230-024-10212-w","DOI":"10.1007\/s11230-024-10212-w"},{"key":"2026052710154625430_j_helm-2025-0025_ref_003","doi-asserted-by":"crossref","unstructured":"Amin, O.M., Chaudhary, A., Caracciolo, M.E., Rubtsova, N.Yu., Wendt, C., Kuzmina, T.A., de Souza, W., Singh, H.S. (2025c): Morphological and molecular description of Corynosoma paraevae n. sp. (Acanthocephala: Polymorphidae) juveniles from Notothenia coriiceps Richardson (Perciformes: Nototheniidae) in Argentine Islands, West Antarctica. Syst Parasitol, 102: 43. DOI: 10.1007\/s1230-025-10238-8","DOI":"10.1007\/s11230-025-10238-8"},{"key":"2026052710154625430_j_helm-2025-0025_ref_004","doi-asserted-by":"crossref","unstructured":"Amin, O.M., Heckmann, R.A. (2017): Neoandracantha peruensis n. gen. n. sp. (Acanthocephala: Polymorphidae) described from cystacanths infecting the ghost crab Ocypode gaudichaudii on the Peruvian coast. Parasite, 24: 40. DOI: 10.1051\/parasite\/2017038","DOI":"10.1051\/parasite\/2017038"},{"key":"2026052710154625430_j_helm-2025-0025_ref_005","doi-asserted-by":"crossref","unstructured":"Amin, O.M., Heckmann, R.A., Bannai, M.A. (2018): Cavisoma magnum (Cavisomidae), a unique Pacific acanthocephalan redescribed from an unusual host, Mugil cephalus (Mugilidae), in the Arabian Gulf, with notes on histopathology and metal analysis. Parasite, 25: 5. DOI: 10.1051\/parasite\/2018006","DOI":"10.1051\/parasite\/2018006"},{"key":"2026052710154625430_j_helm-2025-0025_ref_006","unstructured":"Amin, O.M., Heckmann, R.A., Rubtsova, N. (2025b): Developmental changes and distribution of chemicals in elemental analysis of acanthocephalan hooks using EDXA and EDAX-TEAM systems. Sci Parasitol, 26(1-2): 28 \u2013 50"},{"key":"2026052710154625430_j_helm-2025-0025_ref_007","doi-asserted-by":"crossref","unstructured":"Amin, O.M., Lisitsyna, O., Heckmann, R.A. (2022): Evaluating energy dispersive X-ray analysis (EDXA) as a diagnostic tool in acanthocephalan taxonomy as evidenced in Palaeacanthocephala and Archiacanthocephala. Syst Parasitol, 100: 43 \u2013 57. DOI: 10.1007\/s11230-022-10069-x","DOI":"10.1007\/s11230-022-10069-x"},{"key":"2026052710154625430_j_helm-2025-0025_ref_008","doi-asserted-by":"crossref","unstructured":"Amin, O.M., Rodr\u00edguez, S.M., Farrer, S., Fierro, P., Garc\u00e9s, C., Rivera, F., D\u2019El\u00eda, G. (2023): Review of the concept of Profilicollis Meyer, 1931 with a description of Profilicollis rancoensis n. sp. (Acanthocephala: Polymorphidae) from the freshwater crab, Aegla abtao Schmitt, 1942 (Decapoda: Anomura) in Chile, with a key to congeneric species. Parasite, 30: 42. DOI: 10.1051\/parasite\/2023042","DOI":"10.1051\/parasite\/2023042"},{"key":"2026052710154625430_j_helm-2025-0025_ref_009","doi-asserted-by":"crossref","unstructured":"Goldstein, J. (2003): Scanning Electron Microscopy and X-Ray Microanalysis. Springer. ISBN 978-0-306-47292-3. Retrieved 26 May 2012.","DOI":"10.1007\/978-1-4615-0215-9"},{"key":"2026052710154625430_j_helm-2025-0025_ref_010","doi-asserted-by":"crossref","unstructured":"Heckmann, R.A., Amin, O.M., Standing, M.D. (2007): Chemical analysis of metals in acanthocephalans using energy dispersive X-ray analysis (EDXA, XEDS) in conjunction with a scanning electron microscope (SEM). Comp Parasitol, 74(2): 388 \u2013 391. DOI: 10.1654\/4258.1","DOI":"10.1654\/4258.1"},{"key":"2026052710154625430_j_helm-2025-0025_ref_011","unstructured":"Heckmann, R.A., Amin, O.M., Radwan, N.A.E., Standing, M.D., Eggett, D.L., El Naggar, A.M. (2012): Fine structure and energy dispersive X-ray analysis (EDXA) of the proboscis hooks of Rhadinorynchus ornatus, Van Cleave 1918 (Rhadinorynchidae: Acanthocephala). Sci Parasitol, 13(1): 37 \u2013 43"},{"key":"2026052710154625430_j_helm-2025-0025_ref_012","doi-asserted-by":"crossref","unstructured":"Scimeca, M., Bischetti, S., Lamsira, H.K., Bonfiglio, R., Bonanno, E. (2018): Energy Dispersive X-ray (EDX) microanalysis: A powerful tool in biomedical research and diagnosis. Eur J Histochem, 62(1): 2841. DOI: 10.4081\/ejh.2018.2841","DOI":"10.4081\/ejh.2018.2841"},{"key":"2026052710154625430_j_helm-2025-0025_ref_013","doi-asserted-by":"crossref","unstructured":"Standing, M.D., Heckmann, R.A. 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Retrieved from https:\/\/www.nano.pitt.edu\/sites\/default\/files\/Equipment-SOP\/Guide %20to %20operate %20the %20FEI %20 Scios%20FIB-SEM_TEM%20specimen%20preparation%20V2.pdf"}],"container-title":["Helminthologia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/reference-global.com\/pdf\/10.2478\/helm-2025-0025","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T10:15:59Z","timestamp":1779876959000},"score":1,"resource":{"primary":{"URL":"https:\/\/reference-global.com\/article\/10.2478\/helm-2025-0025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,1]]},"references-count":15,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,11,26]]},"published-print":{"date-parts":[[2025,9,1]]}},"alternative-id":["10.2478\/helm-2025-0025"],"URL":"https:\/\/doi.org\/10.2478\/helm-2025-0025","relation":{},"ISSN":["1336-9083"],"issn-type":[{"value":"1336-9083","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,1]]}}}