{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T11:03:32Z","timestamp":1779879812642,"version":"3.53.1"},"reference-count":21,"publisher":"Walter de Gruyter GmbH","issue":"3","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,9,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Domestic and wild animals can contract amphistomosis, a disease caused by digenetic trematodes belonging to the superfamily Paramphistomoidea. The importance of these flukes is underestimated worldwide due to their ubiquity and abundance among hosts.\n                    <jats:italic>Pseudodiscus collinsi<\/jats:italic>\n                    is a member of the family Paramphistomatidae that infects the colon of equines and elephants. In the present study, the flukes were recovered from the colon of a dead wild Indian elephant (\n                    <jats:italic>Elephas maximus indicus<\/jats:italic>\n                    ). The flukes were stained using acetyl alum carmine and morphologically identified as\n                    <jats:italic>P. collinsi<\/jats:italic>\n                    based on the presence of oral pouches and position of the testes. The polymerase chain reaction (PCR) for amplification of the internal transcribed spacer 2 (ITS-2) region and sequence analysis were performed. The phylogenetic analysis using the Maximum Likelihood (ML) method, based on the Kimura 2-parameter model, revealed the separation of\n                    <jats:italic>P. collinsi<\/jats:italic>\n                    (elephant) as a distinct species from the other amphistomes of different hosts. This is the first molecular marker of\n                    <jats:italic>P. collinsi<\/jats:italic>\n                    to be presented.\n                  <\/jats:p>","DOI":"10.2478\/helm-2025-0029","type":"journal-article","created":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T14:04:14Z","timestamp":1764165854000},"page":"254-258","source":"Crossref","is-referenced-by-count":1,"title":["Molecular identification of\n                    <i>Pseudodiscus collinsi<\/i>\n                    from wild Indian elephant (\n                    <i>Elephas maximus indicus<\/i>\n                    ) based on ITS-2"],"prefix":"10.2478","volume":"62","author":[{"given":"A.","family":"Anaswara","sequence":"first","affiliation":[{"name":"Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University , Wayanad , Kerala , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Arun","sequence":"additional","affiliation":[{"name":"Assistant Forest Veterinary Offi cer , Kozhikode Forest Division India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Geethu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University , Wayanad , Kerala , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. C.","family":"Sreenidhi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University , Wayanad , Kerala , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Suriya","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University , Wayanad , Kerala , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Harshit","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University , Wayanad , Kerala , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. K.","family":"Binoy","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University , Wayanad , Kerala , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Anju","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University , Wayanad , Kerala , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. K.","family":"Deepa","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Pookode, Kerala Veterinary and Animal Sciences University , Wayanad , Kerala , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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