{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:35:03Z","timestamp":1759941303754},"reference-count":64,"publisher":"Centre for Evaluation in Education and Science (CEON\/CEES)","issue":"4","license":[{"start":{"date-parts":[[2019,7,30]],"date-time":"2019-07-30T00:00:00Z","timestamp":1564444800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,30]]},"abstract":"<jats:title>Summary<\/jats:title>\n<jats:sec id=\"j_jomb-2018-0045_s_005\">\n<jats:title>Background<\/jats:title>\n<jats:p>This study aimed to show the status of thioldisulphide homeostasis in essential thrombocytosis patients, which is known to play a role in platelet function.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"j_jomb-2018-0045_s_006\">\n<jats:title>Methods<\/jats:title>\n<jats:p>The study included 27 ET patients and a control group of 36 healthy subjects. Serum total (\u2013SH + \u2013S\u2013S\u2013) and native (\u2013SH) thiol levels were measured in all subjects using an automatic method.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"j_jomb-2018-0045_s_007\">\n<jats:title>Results<\/jats:title>\n<jats:p>Age and gender distribution were similar in both groups. Compared with the control group, in the ET group, there were increased native thiol and total thiol levels (p = 0.001, p = 0.046). There was no correlation between thiol, total thiol and disulphide ratios with Jak2 mutation, hemorrhage and thrombosis. A positive correlation was determined between thrombosis and thiol disulphide homeostasis (p = 0.058). The study results showed that thiol-disulphide homeostasis shifted to the proliferative side in ET, in which ineffective erythropoiesis was predominant. It is also known that platelets are more active in ET cases and thiol disulphide balance is important in platelet function.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"j_jomb-2018-0045_s_008\">\n<jats:title>Conclusions<\/jats:title>\n<jats:p>This result suggests that thrombotic complications may be reduced if the formation is achieved of mechanisms (oxidation mechanisms) that will trigger the increase of disulphide groups. However, more extensive research is needed on this subject.<\/jats:p><\/jats:sec>","DOI":"10.2478\/jomb-2018-0045","type":"journal-article","created":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T09:30:43Z","timestamp":1547112643000},"page":"475-480","source":"Crossref","is-referenced-by-count":5,"title":["Thiol-disulphide homeostasis in essential thrombocythemia patients"],"prefix":"10.5937","volume":"38","author":[{"given":"Aysun \u015eent\u00fcrk","family":"Y\u0131k\u0131lmaz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u015eule Mine","family":"Bakanay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sema","family":"Akinci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murat","family":"Alisik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d6zcan","family":"Erel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u0130mdat","family":"Dilek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"3964","reference":[{"key":"ref571","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/0003-2697(92)90456-H","article-title":"Determination of the in vivo redox status of cysteine, cysteinylglycine, homocysteine, and glutathione in human plasma","volume":"200","year":"1992","journal-title":"Anal Biochem"},{"key":"ref471","first-page":"71","article-title":"Bleeding time and platelet function in essential thrombocythemia and other myeloproliferative syndromes","volume":"1","year":"1996","journal-title":"Leuk Lymphoma"},{"key":"ref481","first-page":"67","article-title":"Pathophysiology and treatment of platelet-mediated microvascular disturbances, major thrombosis and bleeding complications in essential thrombocythaemia and polycythaemia vera","volume":"15","year":"2004","journal-title":"Platelets"},{"key":"ref591","first-page":"69","article-title":"Molecular and cellular aspects of thiol\u2013 disulfide exchange","volume":"63","year":"1990","journal-title":"Adv Enzymol Relat Areas Mol Biol"},{"key":"ref581","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1136\/gut.29.9.1153","article-title":"Glutathione deficiency in alcoholics: risk factor for paracetamol hepatotoxicity","volume":"29","year":"1988","journal-title":"Gut"},{"key":"ref501","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/j.1582-4934.2001.tb00140.x","article-title":"Abnormalities of platelet aggregation in chronic myeloproliferative disorders","volume":"5","year":"2001","journal-title":"J Cell Mol Med"},{"key":"ref91","doi-asserted-by":"crossref","first-page":"S79","DOI":"10.1080\/10715762.2016.1235787","article-title":"Dynamic thiol\/disulphide homeostasis before and after radical prostatectomy in patients with prostate cancer","volume":"50","year":"2016","journal-title":"Free Radic Res"},{"key":"ref211","first-page":"873","article-title":"Platelet abnormalities in myeloproliferative disorders","volume":"10","year":"1990","journal-title":"Clin Lab Med"},{"key":"ref171","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.bcmd.2005.12.031","article-title":"Thrombotic complications in essential thrombocythemia (ET): clinical facts and biochemical riddles","volume":"36","year":"2006","journal-title":"Blood Cells Mol Dis"},{"key":"ref631","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1182\/blood.V98.8.2432","article-title":"A point mutation in the cysteine-rich domain of glycoprotein (GP) IIIa results in the expression of a GPIIb-IIIa (alphaIIbbeta3) integrin receptor locked in a high-affinity state and a Glanzmann thrombasthenia-like phenotype","volume":"98","year":"2001","journal-title":"Blood"},{"key":"ref191","first-page":"22","article-title":"Molecular Basis of Thrombophilia \/ moleku larne osnove trombofilije","volume":"33","year":"2013","journal-title":"Journal of Medical Biochemistry"},{"key":"ref441","doi-asserted-by":"crossref","first-page":"306","DOI":"10.4274\/balkanmedj.2017.1140","article-title":"The Role of Follicular Fluid Thiol\/Disulphide Homeostasis in Polycystic Ovary Syndrome","volume":"35","year":"2018","journal-title":"Balkan Med J"},{"key":"ref281","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1042\/bst0020873","article-title":"Evidence for two populations of disulfide bonds on blood platelets","volume":"2","year":"1974","journal-title":"Biochem Soc Trans"},{"key":"ref401","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1016\/j.freeradbiomed.2009.08.021","article-title":"Measuring the poise of thiol\/disulphide couples in vivo","volume":"47","year":"2009","journal-title":"Free Radic Biol Med"},{"key":"ref531","first-page":"873","article-title":"Platelet abnormalities in myeloproliferative disorders","volume":"10","year":"1990","journal-title":"Clin Lab Med"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"22","DOI":"10.4065\/76.1.22","article-title":"A longterm retrospective study of young women with essential thrombocythemia","volume":"76","year":"2001","journal-title":"Mayo Clin Proc"},{"key":"ref311","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1182\/blood.V98.8.2432","article-title":"A point mutation in the cysteine-rich domain of glycoprotein (GP) IIIa results in the expression of a GPIIb-IIIa (alphaIIbbeta3) integrin receptor locked in a high-affinity state and a Glanzmann thrombasthenia-like phenotype","volume":"98","year":"2001","journal-title":"Blood"},{"key":"ref361","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.ctrv.2008.07.004","article-title":"Elevated copper and oxidative stress in cancer cells as a target for cancer treatment","volume":"35","year":"2009","journal-title":"Cancer Treat Rev"},{"key":"ref541","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0090-6980(74)90076-8","article-title":"Inhibition by sulfhydryl agents of arachidonic acid-induced platelet aggregation and release of potential inflammatory substances","volume":"8","year":"1974","journal-title":"Prostaglandins"},{"key":"ref41","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.ctrv.2008.07.004","article-title":"Elevated copper and oxidative stress in cancer cells as a target for cancer treatment","volume":"35","year":"2009","journal-title":"Cancer Treat Rev"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"6070","DOI":"10.1021\/bi002454e","article-title":"Protein disulfide isomerase and sulfhydryl-dependent pathways in platelet activation","volume":"40","year":"2001","journal-title":"Biochemistry"},{"key":"ref621","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1182\/blood-2002-06-1646","article-title":"Enzymatically catalyzed disulfide exchange is required for platelet adhesion to collagen via integrin 2 1","volume":"102","year":"2003","journal-title":"Blood"},{"key":"ref341","doi-asserted-by":"crossref","first-page":"22","DOI":"10.4065\/76.1.22","article-title":"A longterm retrospective study of young women with essential thrombocythemia","volume":"76","year":"2001","journal-title":"Mayo Clin Proc"},{"key":"ref141","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.clinbiochem.2014.09.026","article-title":"A novel and automated assay for thiol\/disulphide homeostasis","volume":"47","year":"2014","journal-title":"Clin Biochem"},{"key":"ref11","doi-asserted-by":"crossref","first-page":"2658","DOI":"10.1002\/1097-0142(19910515)67:10<2658::AID-CNCR2820671042>3.0.CO;2-C","article-title":"Life expectancy of patients with chronic nonleukemic myeloproliferative disorders","volume":"67","year":"1991","journal-title":"Cancer"},{"key":"ref491","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.bcmd.2005.12.031","article-title":"Thrombotic complications in essential thrombocythemia (ET): clinical facts and biochemical riddles","volume":"36","year":"2006","journal-title":"Blood Cells Mol Dis"},{"key":"ref161","first-page":"67","article-title":"Pathophysiology and treatment of platelet-mediated microvascular disturbances, major thrombosis and bleeding complications in essential thrombocythaemia and polycythaemia vera","volume":"15","year":"2004","journal-title":"Platelets"},{"key":"ref261","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1136\/gut.29.9.1153","article-title":"Glutathione deficiency in alcoholics: risk factor for paracetamol hepatotoxicity","volume":"29","year":"1988","journal-title":"Gut"},{"key":"ref271","first-page":"69","article-title":"Molecular and cellular aspects of thiol\u2013 disulfide exchange","volume":"63","year":"1990","journal-title":"Adv Enzymol Relat Areas Mol Biol"},{"key":"ref131","doi-asserted-by":"crossref","first-page":"126","DOI":"10.2478\/v10011-011-0045-z","article-title":"Role of Redox Metals, Oxidative Protein Products and Antioxidant Potentials of Thiols in Diabetic Retinopathy","volume":"31","year":"2012","journal-title":"Journal of Medical Biochemistry"},{"key":"ref411","doi-asserted-by":"crossref","first-page":"S79","DOI":"10.1080\/10715762.2016.1235787","article-title":"Dynamic thiol\/disulphide homeostasis before and after radical prostatectomy in patients with prostate cancer","volume":"50","year":"2016","journal-title":"Free Radic Res"},{"key":"ref391","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/bj3240001","article-title":"Biochemistry and pathology of radical-mediated protein oxidation","volume":"324","year":"1997","journal-title":"Biochem J"},{"key":"ref151","first-page":"71","article-title":"Bleeding time and platelet function in essential thrombocythemia and other myeloproliferative syndromes","volume":"1","year":"1996","journal-title":"Leuk Lymphoma"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1042\/bj3240001","article-title":"Biochemistry and pathology of radical-mediated protein oxidation","volume":"324","year":"1997","journal-title":"Biochem J"},{"key":"ref551","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1055\/s-0038-1650579","article-title":"Erythromelalgia in essential thrombocythemia is characterized by platelet activation and endothelial cell damage but not by thrombin generation","volume":"76","year":"1996","journal-title":"Thromb Haemost"},{"key":"ref01","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1002\/1097-0142(19900801)66:3<549::AID-CNCR2820660324>3.0.CO;2-6","article-title":"Clinical course of essential thrombocythemia in 147 cases","volume":"66","year":"1990","journal-title":"Cancer"},{"key":"ref451","doi-asserted-by":"crossref","first-page":"126","DOI":"10.2478\/v10011-011-0045-z","article-title":"Role of Redox Metals, Oxidative Protein Products and Antioxidant Potentials of Thiols in Diabetic Retinopathy","volume":"31","year":"2012","journal-title":"Journal of Medical Biochemistry"},{"key":"ref321","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1002\/1097-0142(19900801)66:3<549::AID-CNCR2820660324>3.0.CO;2-6","article-title":"Clinical course of essential thrombocythemia in 147 cases","volume":"66","year":"1990","journal-title":"Cancer"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1016\/j.freeradbiomed.2009.08.021","article-title":"Measuring the poise of thiol\/disulphide couples in vivo","volume":"47","year":"2009","journal-title":"Free Radic Biol Med"},{"key":"ref241","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1089\/1523086041361622","article-title":"The role of thiols and disulfides in platelet function","volume":"6","year":"2004","journal-title":"Antioxid Redox Signal"},{"key":"ref201","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/0049-3848(95)00064-X","article-title":"Arachidonic acid metabolism in platelets of patients with essential thrombocythaemia","volume":"78","year":"1995","journal-title":"Thromb Res"},{"key":"ref301","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1182\/blood-2002-06-1646","article-title":"Enzymatically catalyzed disulfide exchange is required for platelet adhesion to collagen via integrin 2 1","volume":"102","year":"2003","journal-title":"Blood"},{"key":"ref121","doi-asserted-by":"crossref","first-page":"306","DOI":"10.4274\/balkanmedj.2017.1140","article-title":"The Role of Follicular Fluid Thiol\/Disulphide Homeostasis in Polycystic Ovary Syndrome","volume":"35","year":"2018","journal-title":"Balkan Med J"},{"key":"ref291","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1515\/jomb-2017-0053","article-title":"Lipid peroxidation, antioxidative defense and level of 8-hydroxy-2-deoxyguanosine in cervical cancer patients","volume":"37","year":"2018","journal-title":"J Med Biochem"},{"key":"ref421","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1080\/13510002.2016.1180100","article-title":"Assessment of serum thiol\/disulfide homeostasis in multiple myeloma patients by a new method","volume":"22","year":"2017","journal-title":"Redox Rep"},{"key":"ref611","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1515\/jomb-2017-0053","article-title":"Lipid peroxidation, antioxidative defense and level of 8-hydroxy-2-deoxyguanosine in cervical cancer patients","volume":"37","year":"2018","journal-title":"J Med Biochem"},{"key":"ref221","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/0090-6980(74)90076-8","article-title":"Inhibition by sulfhydryl agents of arachidonic acid-induced platelet aggregation and release of potential inflammatory substances","volume":"8","year":"1974","journal-title":"Prostaglandins"},{"key":"ref431","first-page":"248","article-title":"The relationship between low thiol levels and major adverse cardiovascular events after primary percutaneous coronary intervention in patients with STEMI","volume":"46","year":"2018","journal-title":"Turk Kardiyol Dern Ars"},{"key":"ref601","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1042\/bst0020873","article-title":"Evidence for two populations of disulfide bonds on blood platelets","volume":"2","year":"1974","journal-title":"Biochem Soc Trans"},{"key":"ref111","first-page":"248","article-title":"The relationship between low thiol levels and major adverse cardiovascular events after primary percutaneous coronary intervention in patients with STEMI","volume":"46","year":"2018","journal-title":"Turk Kardiyol Dern Ars"},{"key":"ref31","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1515\/jomb-2017-0040","article-title":"Association between oxidative stress and melanoma progression","volume":"37","year":"2018","journal-title":"J Med Biochem"},{"key":"ref231","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1055\/s-0038-1650579","article-title":"Erythromelalgia in essential thrombocythemia is characterized by platelet activation and endothelial cell damage but not by thrombin generation","volume":"76","year":"1996","journal-title":"Thromb Haemost"},{"key":"ref351","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1515\/jomb-2017-0040","article-title":"Association between oxidative stress and melanoma progression","volume":"37","year":"2018","journal-title":"J Med Biochem"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1080\/10428190902802323","article-title":"Study of antioxidant levels in patients with multiple myeloma","volume":"50","year":"2009","journal-title":"Leukemia Lymphoma"},{"key":"ref561","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1089\/1523086041361622","article-title":"The role of thiols and disulfides in platelet function","volume":"6","year":"2004","journal-title":"Antioxid Redox Signal"},{"key":"ref511","first-page":"22","article-title":"Molecular Basis of Thrombophilia \/ moleku larne osnove trombofilije","volume":"33","year":"2013","journal-title":"Journal of Medical Biochemistry"},{"key":"ref101","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1080\/13510002.2016.1180100","article-title":"Assessment of serum thiol\/disulfide homeostasis in multiple myeloma patients by a new method","volume":"22","year":"2017","journal-title":"Redox Rep"},{"key":"ref521","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/0049-3848(95)00064-X","article-title":"Arachidonic acid metabolism in platelets of patients with essential thrombocythaemia","volume":"78","year":"1995","journal-title":"Thromb Res"},{"key":"ref181","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1111\/j.1582-4934.2001.tb00140.x","article-title":"Abnormalities of platelet aggregation in chronic myeloproliferative disorders","volume":"5","year":"2001","journal-title":"J Cell Mol Med"},{"key":"ref371","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1080\/10428190902802323","article-title":"Study of antioxidant levels in patients with multiple myeloma","volume":"50","year":"2009","journal-title":"Leukemia Lymphoma"},{"key":"ref331","doi-asserted-by":"crossref","first-page":"2658","DOI":"10.1002\/1097-0142(19910515)67:10<2658::AID-CNCR2820671042>3.0.CO;2-C","article-title":"Life expectancy of patients with chronic nonleukemic myeloproliferative disorders","volume":"67","year":"1991","journal-title":"Cancer"},{"key":"ref381","doi-asserted-by":"crossref","first-page":"6070","DOI":"10.1021\/bi002454e","article-title":"Protein disulfide isomerase and sulfhydryl-dependent pathways in platelet activation","volume":"40","year":"2001","journal-title":"Biochemistry"},{"key":"ref251","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/0003-2697(92)90456-H","article-title":"Determination of the in vivo redox status of cysteine, cysteinylglycine, homocysteine, and glutathione in human plasma","volume":"200","year":"1992","journal-title":"Anal Biochem"},{"key":"ref461","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1016\/j.clinbiochem.2014.09.026","article-title":"A novel and automated assay for thiol\/disulphide homeostasis","volume":"47","year":"2014","journal-title":"Clin Biochem"}],"container-title":["Journal of Medical Biochemistry"],"original-title":[],"link":[{"URL":"http:\/\/content.sciendo.com\/view\/journals\/jomb\/38\/4\/article-p475.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/scindeks-clanci.ceon.rs\/data\/pdf\/1452-8258\/2019\/1452-82581904475S.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T19:22:05Z","timestamp":1588879325000},"score":1,"resource":{"primary":{"URL":"https:\/\/scindeks.ceon.rs\/article.aspx?artid=1452-82581904475S"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,30]]},"references-count":64,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.2478\/jomb-2018-0045","relation":{},"ISSN":["1452-8266"],"issn-type":[{"value":"1452-8266","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,30]]}}}