{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T23:08:46Z","timestamp":1784156926713,"version":"3.55.0"},"reference-count":32,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2022,3,1]],"date-time":"2022-03-01T00:00:00Z","timestamp":1646092800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec id=\"j_jvetres-2022-0010_s_005\">\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially\n                      <jats:italic>M. agalactiae<\/jats:italic>\n                      . The main option for systematic diagnosis under monitoring control programmes is the enzyme-linked immunosorbent assay (ELISA) test.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jvetres-2022-0010_s_006\">\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>\n                      This study was designed to appraise the performance of two commercial indirect ELISA tests using\n                      <jats:italic>M. agalactiae<\/jats:italic>\n                      p48 protein and one using total protein, for antibody detection in small ruminants after natural infection with different\n                      <jats:italic>M. agalactiae<\/jats:italic>\n                      strains. We carried out the test evaluation using sera of confirmed\n                      <jats:italic>M. agalactiae<\/jats:italic>\n                      -positive goats with clinical signs. In addition, test agreement was assessed by kappa between the three commercial ELISA tests.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jvetres-2022-0010_s_007\">\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All three ELISA tests showed high validity scores (Youden\u2019s J: 72.9\u201384%). The sensitivity values for the P48 protein-based tests were 76.9% and 84.6%, and was 79% for the total protein-based test. The specificity of all tests was 100%. In addition, between the total protein-based ELISA test and the other two ELISA tests based on the P48 protein, the agreement was substantial (kappa: 0.762\u20130.763) and the agreement between the latter two tests was almost perfect (kappa: 0.93).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"j_jvetres-2022-0010_s_008\">\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The validity parameters for all tests allowed their application for diagnostic purposes in lactating goats excreting\n                      <jats:italic>M. agalactiae<\/jats:italic>\n                      in milk and presenting clinical signs. The agreements show that any of these ELISA tests could be equally well used for diagnosis in programmes against CA.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.2478\/jvetres-2022-0010","type":"journal-article","created":{"date-parts":[[2022,3,12]],"date-time":"2022-03-12T08:28:06Z","timestamp":1647073686000},"page":"95-101","source":"Crossref","is-referenced-by-count":4,"title":["Comparison of commercial enzyme-linked immunosorbent assays for diagnosis of contagious agalactia caused by\n                    <i>Mycoplasma agalactiae<\/i>"],"prefix":"10.2478","volume":"66","author":[{"given":"Antonio","family":"S\u00e1nchez","sequence":"first","affiliation":[{"name":"Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, University of Murcia , Murcia , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Contreras","sequence":"additional","affiliation":[{"name":"Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, University of Murcia , Murcia , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda L.","family":"S\u00e1nchez-Corral","sequence":"additional","affiliation":[{"name":"Servicio de Sanidad Animal de la Direcci\u00f3n General de Producci\u00f3n Agropecuaria de la Consejer\u00eda de Agricultura, Ganader\u00eda y Desarrollo Rural de la Junta de Castilla y Le\u00f3n , Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Mart\u00ednez-Nista","sequence":"additional","affiliation":[{"name":"Servicio de Sanidad Animal de la Direcci\u00f3n General de Producci\u00f3n Agropecuaria de la Consejer\u00eda de Agricultura, Ganader\u00eda y Desarrollo Rural de la Junta de Castilla y Le\u00f3n , Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soledad","family":"Collado","sequence":"additional","affiliation":[{"name":"S.G. Sanidad e Higiene Animal y Trazabilidad, Ministerio de Agricultura, Pesca y Alimentaci\u00f3n , Madrid , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 L.","family":"S\u00e1ez","sequence":"additional","affiliation":[{"name":"S.G. Sanidad e Higiene Animal y Trazabilidad, Ministerio de Agricultura, Pesca y Alimentaci\u00f3n , Madrid , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Minguez","sequence":"additional","affiliation":[{"name":"Servicio de Sanidad Animal de la Direcci\u00f3n General de Producci\u00f3n Agropecuaria de la Consejer\u00eda de Agricultura, Ganader\u00eda y Desarrollo Rural de la Junta de Castilla y Le\u00f3n , Le\u00f3n Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian de la","family":"Fe","sequence":"additional","affiliation":[{"name":"Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence \u201cCampus Mare Nostrum\u201d, University of Murcia , Murcia , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2022,3,10]]},"reference":[{"key":"2026042820515104815_j_jvetres-2022-0010_ref_001","doi-asserted-by":"crossref","unstructured":"Amores J., G\u00f3mez-Mart\u00edn \u00c1., Corrales J.C., S\u00e1nchez A., Contreras A., de la Fe C.: Presence of contagious agalactia causing mycoplasmas in Spanish goat artificial insemination centres. Theriogenology 2011, 75, 1265\u20131270, doi: 10.1016\/j.theriogenology.2010.11.040.","DOI":"10.1016\/j.theriogenology.2010.11.040"},{"key":"2026042820515104815_j_jvetres-2022-0010_ref_002","doi-asserted-by":"crossref","unstructured":"Amores J., S\u00e1nchez A., G\u00f3mez-Mart\u00edn \u00c1., Corrales J.C., Contreras A., de la Fe C.: Surveillance of Mycoplasma agalactiae and Mycoplasma mycoides subsp. capri in dairy goat herds. Small Rumin Res 2012, 102, 89\u201393, doi: 10.1016\/j.smallrumres.2011.09.008.","DOI":"10.1016\/j.smallrumres.2011.09.008"},{"key":"2026042820515104815_j_jvetres-2022-0010_ref_003","doi-asserted-by":"crossref","unstructured":"Ariza-Miguel J., Rodr\u00edguez-L\u00e1zaro D., Hern\u00e1ndez M.: A survey of Mycoplasma agalactiae in dairy sheep farms in Spain. 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