{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T08:44:49Z","timestamp":1784709889877,"version":"3.55.0"},"reference-count":64,"publisher":"Polish Academy of Sciences Chancellery","issue":"1","license":[{"start":{"date-parts":[[2014,3,1]],"date-time":"2014-03-01T00:00:00Z","timestamp":1393632000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The presence of tumor within the serosal cavities, often connected with accumulation of serosal effusion, is a quite common problem in the small animal veterinary medicine. The first step in diagnosis of such cases is cytopathological examination. The aim of the present study was to evaluate the usefulness of cytology and immunocytochemistry, using commercially available antibodies (anti-cytokeratin, anti-vimentin, and anti-desmin), in differential diagnosis of malignant tumors located within serosal cavities in dogs. The final cytological diagnosis of carcinoma\/adenocarcinoma, sarcoma, and mesothelioma was obtained on the basis of routine cytopathology and immunocytochemistry, and then confirmed by histopathology and immunohistochemistry. Cytoplasmic immunoreactivitiy of normal mesothelid cells and cytoplasmic immunoreactivity of hyperplastic mesothelial cells revealed constant and strong expression of all examined intermediate filaments: cytokeratin, vimentin and desmin. Application of routine cytopathology and immunocytochemistry allowed 32 neoplastic tumors to be detected: 19 cases of carcinomas\/adenocarcinomas, 6 cases of sarcomas, 7 cases of mesotheliomas. Immunostaining of cytopathological samples with chosen set of antibodies: anti-cytokeratin, anti-vimentin, anti-desmin is a useful, and low invasive test for differentiation between mesotheliomas and carcinomas\/adenocarcinomas in dogs.<\/jats:p>","DOI":"10.2478\/pjvs-2014-0020","type":"journal-article","created":{"date-parts":[[2014,9,3]],"date-time":"2014-09-03T15:14:47Z","timestamp":1409757287000},"page":"149-159","source":"Crossref","is-referenced-by-count":14,"title":["Using of immunocytochemistry in differential diagnosis of neoplasms of serosal cavities in dogs"],"prefix":"10.24425","volume":"17","author":[{"given":"R.","family":"Prze\u017adziecki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Sapierzy\u0144ski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"37468","reference":[{"key":"ref371","first-page":"1997","article-title":"Camps Evaluation of intracavitary mitoxantrone and carboplatin for treatment of carcinomatosis sarcomatosis and mesothelioma with or without malignant effusions retrospective analysis of cases","volume":"12","author":"Charney","year":"2005","journal-title":"Vet Comp Oncol"},{"key":"ref311","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1177\/104063871102300125","article-title":"Malignant mesothelioma of the tunica vaginalis testis in a dog : histological and immunohistochemical characterization","volume":"23","author":"VascellariM","year":"2011","journal-title":"J Vet Diagn Invest"},{"key":"ref631","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1177\/104063871102300125","article-title":"Malignant mesothelioma of the tunica vaginalis testis in a dog : histological and immunohistochemical characterization","volume":"23","author":"VascellariM","year":"2011","journal-title":"J Vet Diagn Invest"},{"key":"ref11","first-page":"229","article-title":"Portal site metastasis of invasive mesothelioma after diagnostic thoracoscopy in a dog","author":"Brisson","year":"2006","journal-title":"J Am Vet Med Assoc"},{"key":"ref331","first-page":"229","article-title":"Portal site metastasis of invasive mesothelioma after diagnostic thoracoscopy in a dog","author":"Brisson","year":"2006","journal-title":"J Am Vet Med Assoc"},{"key":"ref261","first-page":"184","article-title":"Diagnostic value of pericardial fluid analysis in the dog","author":"Sisson","year":"1984","journal-title":"J Am Vet Med Assoc"},{"key":"ref151","first-page":"37","article-title":"Immunocytochemical differentiation of canine mesenchymal tumors in cytologic imprint preparations Vet","author":"H\u00f6inghaus","year":"2008","journal-title":"Clin"},{"key":"ref01","first-page":"161","article-title":"and peritoneal fluids In eds Color Atlas of Cytology of the Dog and Cat St pp","author":"Baker","year":"2000"},{"key":"ref191","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1111\/j.1439-0531.2008.01243.x","article-title":"and immunohistochemical exam in one case of canine endometrial adenocarcinoma","author":"Pires MA","year":"2010","journal-title":"Reprod Domest Anim"},{"key":"ref291","first-page":"57","article-title":"comparative evaluation of immunohistochemical markers for the differential diagnosis between malignant mesothelioma non - 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