{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T21:57:27Z","timestamp":1694210247067},"reference-count":58,"publisher":"Polish Academy of Sciences Chancellery","issue":"3","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n\t\t\t\t<jats:p> Mycobacterium avium ssp. paratuberculosis (MAP) is the cause of chronic gastroenteritis in cattle called the Johne\u2019s disease (JD). The disease causes significant economic losses in cattle production. MAP is also supposed to be involved in the Crohn\u2019s disease and inflammatory bowel disease (IBD) in people. The detection of the cattle infection based on investigations of milk samples and evaluation of the capacity of the methods used to detect the disease was the objective of the present study. Following methods were applied for milk samples testing: detection of MAP in bacterial culture, detection of the specific IS-900 fragment of MAP in the genetic material isolated directly and detection of MAP antibodies. The results obtained were compared with the \u201cgolden standard\u201d results, i.e. the isolation of MAP from the faeces. PQStat-the program for diagnostic reliability estimation, was used for evaluation of the sensitivity, specificity and predictive value. The method based on detection of the specific IS-900 fragment of MAP in the genetic material isolated directly from milk samples was found to possess the highest sensitivity. Detection of anti-MAP antibodies on the other hand showed the lowest sensitivity. The method of detecting anti-MAP antibodies in milk was the most specific while detection of the IS-900 fragment in the genetic material was the least specific method. These results obtained may serve as a guide to choose the most appropriate method for diagnosis of MAP infections by milk sample testing.<\/jats:p>","DOI":"10.2478\/pjvs-2014-0066","type":"journal-article","created":{"date-parts":[[2015,2,27]],"date-time":"2015-02-27T17:56:01Z","timestamp":1425059761000},"page":"459-463","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of methods for Mycobacterium avium ssp. paratuberculosis detection in milk samples from the cattle herd showing low seroprevalence of Johne\u2019s disease"],"prefix":"10.24425","volume":"17","author":[{"given":"J.","family":"Szteyn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Wiszniewska-\u0141aszczych","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Smoli\u0144ska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"37468","reference":[{"key":"ref371","first-page":"1","article-title":"diagn\u00f3sticas principios me todos para su evaluaci\u00f3n e interpretaci\u00f3n In eds Temas de Zoonosis III st edition Buenos Aires Argentina de Zoonosis","author":"Le\u00f3n","year":"2006"},{"key":"ref501","first-page":"95","article-title":"The effect of decontamination protocols on the numbers of sheep strain Mycobacterium avium subsp paratuberculosis isolated from tissues and faeces","author":"Reddacliff LA","year":"2003","journal-title":"Vet Microbiol"},{"key":"ref431","first-page":"1","article-title":"of prevalences of paratuberculosis in farmed animals in Europe","author":"Nielsen","year":"2009","journal-title":"review Prev Vet Med"},{"key":"ref301","first-page":"900","article-title":"Characterization of IS loci in Mycobacterium avium subsp paratuberculosis and development of multiplex PCR typing","author":"Bull","year":"2000","journal-title":"Microbiology"},{"key":"ref01","first-page":"443","article-title":"B\u00f6ttcher Mycobacterium avium ssp paratuberculosis - combined serological testing and classification of individual animals and herds","author":"Gangl","year":"2004","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"key":"ref91","first-page":"8","article-title":"Evaluation of combined high - efficiency DNA extraction and real - time PCR for detection of Mycobacterium avium subsp paratuberculosis in subclinically infected dairy cattle : comparison with faecal culture , milk real - time PCR and milk ELISA","author":"Logar","year":"2012","journal-title":"BMC Vet Res"},{"key":"ref161","first-page":"276","article-title":"Office International Des Epizooties Manual of Diagnostic Tests and Vaccines for Terrestrial Animals Part II mammals birds bees","year":"2008"},{"key":"ref281","first-page":"484","article-title":"Paratuberculosis infection in a dairy herd in the \u017bu\u0142awy region","author":"Wiszniewska","year":"2009","journal-title":"Med Weter"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1089\/fpd.2007.0028","article-title":"Assessment of the prevalence of Mycobacterium avium subsp paratuberculosis in commercially pasteurized milk","author":"Cerf","year":"2007","journal-title":"Foodborne Pathog Dis"},{"key":"ref411","first-page":"900","article-title":"IS to detect Mycobacterium paratuberculosis in retail supplies of whole pasteurized cow s milk in England and","author":"Millar","year":"1996","journal-title":"Appl Environ Microbiol"},{"key":"ref41","first-page":"77","article-title":"MT Improved detection of Mycobacterium avium subsp paratuberculosis in milk by immunomagnetic PCR","author":"Grant","year":"2000","journal-title":"Vet Microbiol"},{"key":"ref81","first-page":"1","article-title":"diagn\u00f3sticas principios me todos para su evaluaci\u00f3n e interpretaci\u00f3n In eds Temas de Zoonosis III st edition Buenos Aires Argentina de Zoonosis","author":"Le\u00f3n","year":"2006"},{"key":"ref521","first-page":"419","article-title":"- prevalence of bovine Johne s disease in buffaloes and cattle population of North India using indigenous ELISA kit based on native Mycobacterium avium subspecies paratuberculosis Bison type genotype of goat origin Infect","volume":"31","author":"Singh","year":"2008","journal-title":"Microbiol Dis"},{"key":"ref31","first-page":"217","article-title":"MT Paratuberculosis of present knowledge","author":"Collins","year":"2003","journal-title":"review Acta Vet Scand"},{"key":"ref201","first-page":"137","article-title":"JE DO CD Mycobacterium paratuberculosis shedding into milk : association of ELISA seroreactivity with DNA detection on milk Vet","author":"Pinedo","year":"2008","journal-title":"Intern Appl Res Med"},{"key":"ref111","doi-asserted-by":"crossref","first-page":"133","DOI":"10.20506\/rst.20.1.1275","article-title":"MT avium subsp paratuberculosis pathogen pathogenesis diagnosis","volume":"20","author":"Manning","year":"2001","journal-title":"Rev Sci Tech"},{"key":"ref571","first-page":"484","article-title":"Paratuberculosis infection in a dairy herd in the \u017bu\u0142awy region","author":"Wiszniewska","year":"2009","journal-title":"Med Weter"},{"key":"ref341","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S0956-7135(01)00046-9","article-title":"avium subspecies paratuberculosis the nature of the problem","volume":"12","author":"Hermon","year":"2001","journal-title":"Food Control"},{"key":"ref421","first-page":"13","article-title":"Comparison of single - 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round and nested PCR assays targeting IS locus for detection of Mycobacterium avium subsp paratuberculosis","author":"Mobius","year":"2008","journal-title":"Vet Microbiol"},{"key":"ref271","first-page":"77","article-title":"ELISA and fecal culture for paratuberculosis disease sensitivity and specificity of each method","author":"Whitlock","year":"2000","journal-title":"Vet Microbiol"},{"key":"ref261","first-page":"85","article-title":"How accurately can we detect Mycobacterium avium subsp paratuberculosis infection","author":"Timms","year":"2001","journal-title":"J Microbiol Meth"},{"key":"ref561","first-page":"77","article-title":"ELISA and fecal culture for paratuberculosis disease sensitivity and specificity of each method","author":"Whitlock","year":"2000","journal-title":"Vet Microbiol"},{"key":"ref491","first-page":"137","article-title":"JE DO CD Mycobacterium paratuberculosis shedding into milk : association of ELISA seroreactivity with DNA detection on milk Vet","author":"Pinedo","year":"2008","journal-title":"Intern Appl Res Med"},{"key":"ref391","first-page":"71","article-title":"Heat inactivation of Mycobacterium avium subsp paratuberculosis in milk","author":"McDonald","year":"2005","journal-title":"Appl Environ Microbiol"},{"key":"ref51","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S0956-7135(01)00046-9","article-title":"avium subspecies paratuberculosis the nature of the problem","volume":"12","author":"Hermon","year":"2001","journal-title":"Food Control"},{"key":"ref531","doi-asserted-by":"crossref","DOI":"10.1177\/104063870601800102","article-title":"Longitudinal study of ELISA seroreactivity to Mycobacterium avium subsp paratuberculosis in infected cattle and culture - negative herd mates","volume":"18","author":"Sweeney","year":"2006","journal-title":"J Vet Diagn Invest"},{"key":"ref71","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1016\/j.ijid.2008.06.034","article-title":"Association Mycobacterium avium subsp paratuberculosis DNA in blood and cellular and humoral immune response in inflammatory bowel disease patients and controls","volume":"13","author":"Juste","year":"2009","journal-title":"Int J Infect Dis"},{"key":"ref141","first-page":"1","article-title":"of prevalences of paratuberculosis in farmed animals in Europe","author":"Nielsen","year":"2009","journal-title":"review Prev Vet Med"},{"key":"ref311","doi-asserted-by":"crossref","first-page":"433","DOI":"10.1089\/fpd.2007.0028","article-title":"Assessment of the prevalence of Mycobacterium avium subsp paratuberculosis in commercially pasteurized milk","author":"Cerf","year":"2007","journal-title":"Foodborne Pathog Dis"},{"key":"ref321","first-page":"217","article-title":"MT Paratuberculosis of present knowledge","author":"Collins","year":"2003","journal-title":"review Acta Vet Scand"},{"key":"ref291","first-page":"443","article-title":"B\u00f6ttcher Mycobacterium avium ssp paratuberculosis - combined serological testing and classification of individual animals and herds","author":"Gangl","year":"2004","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"key":"ref251","first-page":"11","article-title":"Effectiveness of Mycobacterium paratuberculosis isolation from raw milk by means of direct isolation of DNA and classic culture","author":"Szteyn","year":"2008","journal-title":"Pol J Vet Sci"},{"key":"ref181","first-page":"77","article-title":"Parallel faecal and organ Mycobacterium avium subsp paratuberculosis culture of different productivity types of cattle","author":"Pavlik","year":"2000","journal-title":"Vet Microbiol"},{"key":"ref191","first-page":"900","article-title":"Application of IS for detection of Mycobacterium avium subspecies paratuberculosis directly from raw milk","author":"Pillai","year":"2002","journal-title":"J Dairy Sci"},{"key":"ref171","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0167-5877(99)00037-9","article-title":"- level economic losses associated with Johne s disease on US dairy operations","author":"Ott","year":"1999","journal-title":"Prev Vet Med"},{"key":"ref451","first-page":"276","article-title":"Office International Des Epizooties Manual of Diagnostic Tests and Vaccines for Terrestrial Animals Part II mammals birds bees","year":"2008"},{"key":"ref231","first-page":"419","article-title":"- prevalence of bovine Johne s disease in buffaloes and cattle population of North India using indigenous ELISA kit based on native Mycobacterium avium subspecies paratuberculosis Bison type genotype of goat origin Infect","volume":"31","author":"Singh","year":"2008","journal-title":"Microbiol Dis"}],"container-title":["Polish Journal of Veterinary Sciences"],"original-title":[],"link":[{"URL":"http:\/\/content.sciendo.com\/view\/journals\/pjvs\/17\/3\/article-p459.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/view\/j\/pjvs.2014.17.issue-3\/pjvs-2014-0066\/pjvs-2014-0066.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T14:03:54Z","timestamp":1679493834000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.pan.pl\/dlibra\/publication\/114240\/edition\/99293\/content"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9,1]]},"references-count":58,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.2478\/pjvs-2014-0066","relation":{},"ISSN":["2300-2557"],"issn-type":[{"value":"2300-2557","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9,1]]}}}