{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:58:41Z","timestamp":1790873921774,"version":"4.1.0"},"reference-count":64,"publisher":"Polish Academy of Sciences Chancellery","issue":"4","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cryptosporidium intestinal parasites have been detected in farmed pigs worldwide. Infections are usually asymptomatic with a low number of oocysts shed in pig feces. This makes the recognition of infection difficult or unsuccessful when microscopic methods are used. The aim of this study was molecular identification of Cryptosporidium species in pig herds raised in Poland with regard to the occurrence of zoonotic species. In total, 166 pig fecal samples were tested. The examined pigs were aged 1 to 20 weeks. Overall, 39 pig farms were monitored for parasite presence. The detection and identification of Cryptosporidium DNA was performed on the basis of PCR-RFLP and nucleotide sequence analysis of the amplified 18 SSU rRNA and COWP gene fragments. Infected animals were housed in 21 (53.8%) of the pig farms monitored. The presence of Cryptosporidum was confirmed in 46 (27.7%) samples of pig feces. Among positive fecal samples, 34 (29.3%) were collected from healthy animals, and 12 (24%) from diarrheic pigs. Most infected animals (42.1%) were 2 to 3 months old. The following parasite species were detected: C. scrofarum, C. suis and C. parvum. Indeed, asymptomatic infections caused by C. scrofarum were observed in the majority of the herds. Mixed infections caused by C. suis and C. scrofarum were not common; however, they were observed in 8.6% of the positive animals. C. parvum DNA was found only in one sample collected from a diarrheic pig. The application of molecular diagnostic tools allowed for detection and identification of Cryptosporidium species in pigs. The sporadic findings of C. parvum are subsequent evidence for the contribution of pigs in the transmission of cryptosporidiosis from animals to humans.<\/jats:p>","DOI":"10.2478\/pjvs-2014-0086","type":"journal-article","created":{"date-parts":[[2015,2,27]],"date-time":"2015-02-27T17:56:01Z","timestamp":1425059761000},"page":"577-582","source":"Crossref","is-referenced-by-count":13,"title":["Molecular studies on pig cryptosporidiosis in Poland"],"prefix":"10.24425","volume":"17","author":[{"given":"A.","family":"Rze\u017cutka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Kaupke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Kozyra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Pejsak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"37468","reference":[{"key":"ref221","first-page":"196","article-title":"Occurrence and molecular identification of Cryptosporidium species isolated from cattle in","author":"Rze\u017cutka","year":"2013","journal-title":"Vet Parasitol"},{"key":"ref401","first-page":"85","article-title":"Characterization of Cryptosporidium parvum in human and animal feces by single - 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related infection of Cryptosporidium suis muris and Cryptosporidium pig genotype II in pigs on a farm complex in the Czech Republic","author":"Kv\u00e1\u010d","year":"2009","journal-title":"Vet Parasitol"},{"key":"ref41","first-page":"1","article-title":"General biology In eds cryptosporidiosis Group","author":"Fayer","year":"2008"},{"key":"ref191","first-page":"112","article-title":"Occurrence of Cryptosporidium suis and Cryptosporidium scrofarum on commercial swine farms in the Czech Republic and its associations with age and husbandry practices","author":"N\u0115mejc","year":"2013","journal-title":"Parasitol Res"},{"key":"ref581","first-page":"53","article-title":"Prevalence and pathogenicity of Cryptosporidium suis in pre - and post - weaned pigs","author":"V\u00edtovec","year":"2006","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"key":"ref461","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/s00436-008-1215-x","article-title":"Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Republic","author":"Kv\u00e1\u010d","year":"2009","journal-title":"Parasitol Res"},{"key":"ref161","first-page":"141","article-title":"Cryptosporidium and Giardia in different age groups of Danish cattle and pigs - occurrence and management associated risk factors","author":"Maddox","year":"2006","journal-title":"Vet Parasitol"},{"key":"ref131","first-page":"191","article-title":"Cryptosporidium scrofarum sp Cryptosporidiidae in domestic pigs scrofa","author":"Kv\u00e1\u010d","year":"2013","journal-title":"Vet Parasitol"},{"key":"ref231","first-page":"451","article-title":"Livestock In eds cryptosporidiosis Group","author":"Sant\u00edn","year":"2008"},{"key":"ref321","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00436-008-1179-x","article-title":"Cryptosporidium spp infections in humans animals and the environment in","author":"Bajer","year":"2008","journal-title":"Parasitol Res"},{"key":"ref261","first-page":"53","article-title":"Prevalence and pathogenicity of Cryptosporidium suis in pre - and post - weaned pigs","author":"V\u00edtovec","year":"2006","journal-title":"J Vet Med B Infect Dis Vet Public Health"},{"key":"ref141","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1007\/s00436-008-1215-x","article-title":"Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Republic","author":"Kv\u00e1\u010d","year":"2009","journal-title":"Parasitol Res"},{"key":"ref431","first-page":"119","article-title":"UM Prevalence of Cryptosporidium genotypes in pre and post - weaned pigs in Australia","author":"Johnson","year":"2008","journal-title":"Exp Parasitol"},{"key":"ref81","first-page":"85","article-title":"Characterization of Cryptosporidium parvum in human and animal feces by single - tube nested polymerase chain reaction and restriction analysis","author":"Homan","year":"1999","journal-title":"Parasitol Res"},{"key":"ref01","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00436-008-1179-x","article-title":"Cryptosporidium spp infections in humans animals and the environment in","author":"Bajer","year":"2008","journal-title":"Parasitol Res"},{"key":"ref561","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.pt.2006.02.009","article-title":"for investigating the environmental transmission of Cryptosporidium and Giardia infections in humans","volume":"22","author":"Smith","year":"2006","journal-title":"Tools Trends Parasitol"}],"container-title":["Polish Journal of Veterinary Sciences"],"original-title":[],"link":[{"URL":"https:\/\/content.sciendo.com\/view\/journals\/pjvs\/17\/4\/article-p577.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/view\/j\/pjvs.2014.17.issue-4\/pjvs-2014-0086\/pjvs-2014-0086.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T14:04:00Z","timestamp":1679493840000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.pan.pl\/dlibra\/publication\/114260\/edition\/99313\/content"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,12,1]]},"references-count":64,"journal-issue":{"issue":"4"},"URL":"https:\/\/doi.org\/10.2478\/pjvs-2014-0086","relation":{},"ISSN":["2300-2557"],"issn-type":[{"value":"2300-2557","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,12,1]]}}}