{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T08:31:47Z","timestamp":1786350707510,"version":"build-2736575974"},"reference-count":52,"publisher":"Walter de Gruyter GmbH","issue":"2","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:bold>Introduction<\/jats:bold>\n                    : The present study aimed to assess the presence of main types of microorganisms involved in the aetiopathogenesis of chronic periodontitis with PCR technique and determinates the presence of composite IL-1 genotype and their associations with founded bacteria.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Material and method<\/jats:bold>\n                    : The examined group was consisted from 20 subjects with diagnosed chronic periodontitis and 20 healthy control without periodontitis. Clinical parameters like gingival index (GI), plaque index (PI), bleeding on probing (BOP), periodontal pocket depth (PPD) and clinical attachment lost (CAL) were determinates. Subgingival dental plaque was collected using a sterilized paper point. We used Parodontose Plus test, reverse hybridization kit, for the detection of periodontal marker bacteria, as well as for the detection of composite Interleukin -1 Genotype\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results<\/jats:bold>\n                    : The most present bacterial species detected from subgingival dental plaque was Treponema denticola and Porfiromonas gingivalis which was present in 65% of examined patients. In relation to the presence of positive genotype in patients, there was no significant difference between the test and control group for p&gt; 0.05 (p = 1.00). For \u03c72=8,17 (p=0,06, p&lt;0,05) there is an association between\n                    <jats:italic>Prevotella intermedia<\/jats:italic>\n                    , and composite genotype.\n                  <\/jats:p>\n                  <jats:p>\n                    Between positive genotype and analyzed bacterial species\n                    <jats:italic>A. actinomycetem comitans<\/jats:italic>\n                    for p&gt; 0.05 (p = 1.00),\n                    <jats:italic>P. gingivalis<\/jats:italic>\n                    for p&gt; 0.05 (p = 0.16), T. Forsythia for p&gt; 0.05 (p = 0.20),\n                    <jats:italic>T. Denticola<\/jats:italic>\n                    for p&gt; 0.05 (p = 0.64) no association was found.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion<\/jats:bold>\n                    . This investigations confirmed the strong association of these five examined periopathogenes with periodontitis.\n                  <\/jats:p>","DOI":"10.2478\/prilozi-2019-0019","type":"journal-article","created":{"date-parts":[[2019,10,12]],"date-time":"2019-10-12T05:31:08Z","timestamp":1570858268000},"page":"89-97","source":"Crossref","is-referenced-by-count":3,"title":["Identification of Periopathogenes from Dental Plaque in Periodontal Patients with PCR Technique and Their Association with Composite Interleukin-1 Genotype"],"prefix":"10.2478","volume":"40","author":[{"given":"Aneta Atanasovska","family":"Stojanovska","sequence":"first","affiliation":[{"name":"Faculty of Dentistry \u2013 Ss. Cyril and Methodius University - Skopje, University Dental Clinic \u201cSt. Pantelejmon\u201d , Skopje"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saska","family":"Todoroska","sequence":"additional","affiliation":[{"name":"University Dental Clinic \u201cSt. Pantelejmon\u201d , Skopje"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirjana","family":"Popovska","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry \u2013 Ss. Cyril and Methodius University - Skopje, University Dental Clinic \u201cSt. Pantelejmon\u201d , Skopje"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilijana","family":"Muratovska","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry \u2013 Ss. Cyril and Methodius University - Skopje, University Dental Clinic \u201cSt. Pantelejmon\u201d , Skopje"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda Zendeli","family":"Bedzeti","sequence":"additional","affiliation":[{"name":"University Dental Clinic \u201cSt. Pantelejmon\u201d , Skopje"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","published-online":{"date-parts":[[2019,10,11]]},"reference":[{"key":"#cr-split#-2026081008140131475_j_prilozi-2019-0019_ref_001_w2aab3b7c11b1b6b1ab1ab1Aa.1","doi-asserted-by":"crossref","unstructured":"1. 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The interleukin-1 genotype as a severity factor in adult periodontal disease. J Clin Periodontol 1997; 24(1): 72\u20137.10.1111\/j.1600-051X.1997.tb01187.x","DOI":"10.1111\/j.1600-051X.1997.tb01187.x"},{"key":"2026081008140131475_j_prilozi-2019-0019_ref_004_w2aab3b7c11b1b6b1ab1ab4Aa","doi-asserted-by":"crossref","unstructured":"4. Deppe H, M\u00fccke T, Wagenpfeil S, Kesting M, Karl J, Noe S, Sculean A. Are selected IL-1 polymorphisms and selected subgingival microorganisms significantly associated to periodontitis in type 2 diabetes patients? a clinical study. BMC Oral Health. 2015 Nov 14; 15(1): 143. doi: 10.1186\/s12903-015-0132-5.10.1186\/s12903-015-0132-5465028826576766","DOI":"10.1186\/s12903-015-0132-5"},{"key":"2026081008140131475_j_prilozi-2019-0019_ref_005_w2aab3b7c11b1b6b1ab1ab5Aa","doi-asserted-by":"crossref","unstructured":"5. 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APMIS. 2004 Nov-Dec"},{"key":"#cr-split#-2026081008140131475_j_prilozi-2019-0019_ref_016_w2aab3b7c11b1b6b1ab1ac16Aa.2","unstructured":"112(11-12): 785-807. Molecular genetic methods for the diagnosis of fastidious microorganisms.[APMIS. 2004]"},{"key":"2026081008140131475_j_prilozi-2019-0019_ref_017_w2aab3b7c11b1b6b1ab1ac17Aa","doi-asserted-by":"crossref","unstructured":"17. Veal DA, Deere D, Ferrari B, Piper J, Attfield PV. Fluorescence staining and flow cytometry for monitoring microbial cells. J Immunol Methods 2000; 243: 191\u2013210. [PubMed]10.1016\/S0022-1759(00)00234-9","DOI":"10.1016\/S0022-1759(00)00234-9"},{"key":"2026081008140131475_j_prilozi-2019-0019_ref_018_w2aab3b7c11b1b6b1ab1ac18Aa","doi-asserted-by":"crossref","unstructured":"18. Morikawa M, Chiba T, Tomii N, Sato S, Takahashi Y, Konishi K, Numabe Y, Iwata K, Imai K.Comparative analysis of putative periodontopathic bacteria by multiplex polymerase chain reaction. 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