{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T13:36:34Z","timestamp":1740144994380,"version":"3.37.3"},"reference-count":42,"publisher":"Walter de Gruyter GmbH","issue":"2","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of this study was to investigate if there is specific host-parasite association in Chilean populations of Trypanosoma cruzi. For this purpose, two groups of parasites were analyzed, one from chronic chagasic patients, and the other from Triatoma infestans triatomines in three regions of the country. The first group consisted of four types of samples: parasites from peripheral blood of non-cardiopathic T. cruzi infected patients (NB); parasites from their corresponding xenodiagnosis (NX); parasites from peripheral blood of T. cruzi infected cardiopathic patients (CB) and parasites from their xenodiagnostics (CX). The T. infestans sample in turn was from three regions: III, V and M (Metropolitan). The genetic differentiation by the Fisher exact method, the lineage distribution of the samples, the molecular phylogeny and the frequency of multiclonality were analysed. The results show that not only are the groups of T. cruzi clones from Chagas disease patients and vectors genetically differentiated, but also all the sub-groups (NB, NX, CB and CX) from the III, V and M regions. The analysis of lineage distribution was concordant with the above results, because significant differences among the percentages of TcI, TcIII and hybrids (TcV or TcVI) were observed. The phylogenetic reconstruction with these Chilean T. cruzi samples was coherent with the above results because the four chagasic samples clustered together in a node with high bootstrap support, whereas the three triatomine samples (III, V and M) were located apart from that node. The topology of the tree including published T. cruzi clones and isolates was concordant with the known topology, which confirmed that the results presented here are correct and are not biased by experimental error. Taken together the results presented here are concordant with a specific host-parasite association between some Chilean T. cruzi populations.<\/jats:p>","DOI":"10.2478\/s11686-013-0123-0","type":"journal-article","created":{"date-parts":[[2013,5,10]],"date-time":"2013-05-10T07:13:26Z","timestamp":1368170006000},"source":"Crossref","is-referenced-by-count":5,"title":["Microsatellite loci-based distribution of Trypanosoma cruzi genotypes from Chilean chronic Chagas disease patients and Triatoma infestans is concordant with a specific host-parasite association hypothesis"],"prefix":"10.2478","volume":"58","author":[{"given":"Juan","family":"Venegas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felipe","family":"D\u00edaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamara","family":"Rojas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Miranda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Jercic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Co\u00f1oep\u00e1n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Vargas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Pichuantes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Gajardo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gittith","family":"S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref181","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1590\/S0074-02761999000700025","article-title":"Taxonomy of Trypanosoma cruzi : a Commentary on Characterization and Nomenclature do http dx doi org","volume":"19","author":"Momen","year":"1999","journal-title":"Memorias Instituto Oswaldo Cruz"},{"key":"ref81","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1590\/S0074-02762003000100001","article-title":"Should Trypanosoma cruzi be Called cruzi Complex ? 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Tropica"},{"key":"ref111","doi-asserted-by":"crossref","first-page":"740","DOI":"10.4269\/ajtmh.1999.60.740","article-title":"Utility of the polymerase chain reaction in detection of Trypanosoma cruzi in Guatemalan Chagas disease vectors of and","volume":"12","author":"Dorn","year":"1999","journal-title":"American Journal Tropical Medicine Hygiene"},{"key":"ref151","doi-asserted-by":"publisher","first-page":"7396","DOI":"10.1073\/pnas.12118719.http:\/\/dx.doi.org\/10.1073\/pnas.121187198","article-title":"Nucleotide sequences provide evidence of genetic exchange among distantly related lineages of Trypanosoma cruzi Proceeding of National Academic of","volume":"16","author":"Machado","year":"2001","journal-title":"Science USA"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1038\/368455a0.http:\/\/dx.doi.org\/10.1038\/368455a0","article-title":"High resolution human evolutionary trees with polymorphic microsatellites","volume":"2","author":"Bowcock","year":"1994","journal-title":"Nature"},{"key":"ref191","doi-asserted-by":"publisher","first-page":"3776","DOI":"10.1073\/pnas.95.7.3776","article-title":"Probing the genetic population structure of Trypanosoma cruzi with polymorphic microsatellites Proceeding of National Academic of http dx doi org","volume":"20","author":"Oliveira","year":"1998","journal-title":"Science USA"},{"key":"ref91","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1371\/journal.ppat.0020024.http:\/\/dx.doi.org\/10.1371\/journal.ppat.0020024","article-title":"Ancestral genomes sex , and the population structure of Trypanosoma cruzi","volume":"10","author":"De Freitas","year":"2006","journal-title":"PLOS Pathogens"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.4269\/ajtmh.2006.74.1008","article-title":"Variation in Trypanosoma cruzi clonal composition detected in blood patients and xenodiagnosis triatomines : implications in the molecular epidemiology of Chile of and","volume":"7","author":"Coronado","year":"2006","journal-title":"American Journal Tropical Medicine Hygiene"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/S0020-7519(99)00168-X.http:\/\/dx.doi.org\/10.1016\/S0020-7519(99)00168-X","article-title":"Identification of six Trypanosoma cruzi phylogenetic lineages by random amplified polymorphic DNA and multilocus enzyme electrophoresis for","volume":"5","author":"Brisse","year":"2000","journal-title":"International Journal Parasitology"},{"key":"ref01","doi-asserted-by":"crossref","first-page":"302","DOI":"10.4269\/ajtmh.1987.37.302","article-title":"et Characterization of Trypanosoma cruzi sylvatic transmission cycles and their association with cardiopathy populations by zymodemes : correlation with clinical picture of and","volume":"1","author":"Apt","year":"1987","journal-title":"American Journal Tropical Medicine Hygiene"},{"key":"ref371","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/j.actatropica.2008.11.007.http:\/\/dx.doi.org\/10.1016\/j.actatropica.2008.11.007","article-title":"b Differential distribution of Trypanosama cruzi clones in human chronic cardiopathic and noncardiopathic individuals","volume":"38","author":"Venegas","year":"2009","journal-title":"Acta Tropica"},{"key":"ref21","doi-asserted-by":"crossref","first-page":"335","DOI":"10.4269\/ajtmh.1992.46.335","article-title":"Direct identification of Trypanosoma cruzi natural clones in vectors and mammalian hosts by polymerase chain reaction amplification of and","volume":"3","author":"Breni\u00e8re","year":"1992","journal-title":"American Journal Tropical Medicine Hygiene"},{"key":"ref281","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1007\/s004360050443.http:\/\/dx.doi.org\/10.1007\/s004360050443","article-title":"Hydrophobic attachment of Trypanosoma cruzi to a superficial layer of the rectal cuticle in the bug Triatoma infestans","volume":"29","author":"Schmidt","year":"1998","journal-title":"Parasitology Research"},{"key":"ref301","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/S0166-6851(96)02755-7","article-title":"DNA markers define two major phylogenetic lineages of Trypanosoma cruzi http dx doi org","volume":"31","author":"Souto","year":"1996","journal-title":"Molecular Biochemistry Parasitology"},{"key":"ref241","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1093\/oxfordjournals.jhered.a111573","article-title":"GENEPOP Version population genetics software for exact tests and ecumenicism","volume":"25","author":"Raymond","year":"1995","journal-title":"Journal of Heredity"},{"key":"ref221","doi-asserted-by":"publisher","first-page":"9197","DOI":"10.1073\/pnas.94.17.9197","article-title":"Detecting immigration by using multilocus genotypes Proceeding of National Academic of http dx doi org","volume":"23","author":"Rannala","year":"1997","journal-title":"Science USA"},{"key":"ref331","doi-asserted-by":"publisher","first-page":"277","DOI":"10.2307\/2409232","article-title":"Isozyme variability in Trypanosoma cruzi , the agent of Chagas disease : genetical , taxonomical , and epidemiological significance http dx doi org","volume":"34","author":"Tibayrenc","year":"1988","journal-title":"Evolution"},{"key":"ref311","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/j.exppara.2006.04.005.http:\/\/dx.doi.org\/10.1016\/j.exppara.2006.04.005","article-title":"Trypanosoma cruzi : Sequence analysis of the variable region of kinetoplast minicircles","volume":"32","author":"Telleria","year":"2006","journal-title":"Experimental Parasitology"},{"key":"ref431","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/S0020-7519(97)00178-1.http:\/\/dx.doi.org\/10.1016\/S0020-7519(97)00178-1","article-title":"Molecular epidemiology of American trypanosomiasis in Brazil based on dimorphisms of rRNA and mini - exon gene sequences for","volume":"44","author":"Zingales","year":"1998","journal-title":"International Journal Parasitology"},{"key":"ref261","first-page":"406","article-title":"The neighbor - joining method : A new method for reconstructing phylogenetic trees and","volume":"27","author":"Saitou","year":"1987","journal-title":"Molecular Biology Evolution"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"1319","DOI":"10.1016\/j.ijpara.2007.04.015http:\/\/dx.doi.org\/10.1016\/j.ijpara.2007.04.015","article-title":"Direct molecular profiling of minicircle signatures and lineages of Trypanosoma cruzi bloodstream populations causing congenital Chagas disease for","volume":"6","author":"Burgos","year":"2007","journal-title":"International Journal Parasitology"},{"key":"ref131","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1038\/nature01438.http:\/\/dx.doi.org\/10.1038\/nature01438","article-title":"Mechanism of genetic 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Research"},{"key":"ref341","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1017\/S0031182003004475.http:\/\/dx.doi.org\/10.1017\/S0031182003004475","article-title":"Trypanosoma cruzi isolates from Chile are heterogeneous and composed of mixed populations when characterized by schizodeme and Southern analyses","volume":"35","author":"Torres","year":"2004","journal-title":"Parasitology"},{"key":"ref381","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1007\/s00436-010-1939-2.http:\/\/dx.doi.org\/10.1007\/s00436-010-1939-2","article-title":"Microsatellite marker analysis shows differentiation among Trypanosoma cruzi populations of peripheral blood and dejections of Triatoma infestans fed on the same chronic chagasic patients","volume":"39","author":"Venegas","year":"2010","journal-title":"Parasitology Research"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1006\/expr.1998.4295.http:\/\/dx.doi.org\/10.1006\/expr.1998.4295","article-title":"Diferent behaviour of two Trypanosoma cruzi major clones : transmission and circulation in young Bolivian children","volume":"4","author":"Breniere","year":"1998","journal-title":"Experimental Parasitology"},{"key":"ref201","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1111\/j.1365-294X.1995.tb00227.x","article-title":"Microsatellite analysis of population structure in Canadian polar bears http dx doi org","volume":"21","author":"Paetkau","year":"1995","journal-title":"Molecular Ecology"},{"key":"ref71","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1590\/S0074-02761984000400016.http:\/\/dx.doi.org\/10.1590\/S0074-02761984000400016","article-title":"Trypanosoma cruzi : strain selection by different schedules of mouse passage of an initially mixed infection do","volume":"8","author":"Deane","year":"1984","journal-title":"Memorias Instituto Oswaldo Cruz"},{"key":"ref121","first-page":"164","article-title":"PHYLIP Phylogeny Inference Package ( Version","volume":"13","author":"Felsenstein","year":"1989","journal-title":"Cladistics"},{"key":"ref231","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.2307\/2410454","article-title":"a An exact test for population differentiation http dx doi org","volume":"24","author":"Raymond","year":"1995","journal-title":"Evolution"},{"key":"ref351","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/0001-706X(90)90067-A","article-title":"SubspeciWc kDNA probes for major clones of Trypanosoma cruzi http dx doi org","volume":"36","author":"Veas","year":"1990","journal-title":"Acta Tropica"},{"key":"ref291","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1006\/expr.2001.4607","article-title":"Identification of Trypanosoma cruzi genotypes circulating in Chilean chagasic patients http dx doi org","volume":"30","author":"Solari","year":"2001","journal-title":"Experimental Parasitology"},{"key":"ref441","doi-asserted-by":"publisher","first-page":"1051","DOI":"10.1590\/S0074-02762009000700021.http:\/\/dx.doi.org\/10.1590\/S0074-02762009000700021","article-title":"A new consensus for Trypanosoma cruzi intraspecific nomenclature : second revision meeting recommends TcI to TcVI do","volume":"45","author":"Zingales","year":"2009","journal-title":"Memorias Instituto Oswaldo Cruz"},{"key":"ref391","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1179\/2047773211Y.0000000002.http:\/\/dx.doi.org\/10.1179\/2047773211Y.0000000002","article-title":"Geographical structuring of Trypanosoma cruz populations from Chilean Triatoma infestans triatomines and their genetic relationship with other Latino American counterparts and","volume":"40","author":"Venegas","year":"2011","journal-title":"Annals of Tropical Medicine 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