{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T13:36:35Z","timestamp":1740144995258,"version":"3.37.3"},"reference-count":33,"publisher":"Walter de Gruyter GmbH","issue":"3","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although the incidence of anisakiasis is rising worldwide, its frequency is still unknown in Brazil. The aim of this study was to verify immunoreactivity to Anisakis simplex antigens in healthy adults and determine its possible relationship with frequency of fish consumption and allergy symptoms. A prospective cross-sectional study was carried out with 67 volunteers recruited from a military facility in Niter\u00f3i, Brazil. The subjects completed a structured questionnaire and serum titers of specific anti-Anisakis IgE and IgG antibodies were measured. The association between frequency of fish intake and IgE reactivity was evaluated by Fisher\u2019s exact test. Almost all subjects (97.0%, 65\/67) that consumed seafood; 64.6% (42\/65) ate fish at least once weekly. Of all seafood consumers, 56.9% (37\/65) reported allergy symptoms, being gut allergies most often cited (35.5%). IgE seroreactivity to Anisakis simplex was found in 20.9% of subjects (14\/67), with 13.4% (9\/67) reacting exclusively to somatic antigen, 3.0% (2\/67) exclusively to excretory\/secretory antigens and 4.5% (3\/67) to both antigens. There was a significant association between frequency of fish consumption and positive serology (p = 0.019). An immunoblot assay for Anisakis antigens showed different positive bands for IgG. The direct relationship between ELISA reactivity and frequency of fish intake and absence of association with allergy symptoms suggests previous contact with Anisakis simplex antigens.<\/jats:p>","DOI":"10.2478\/s11686-013-0157-3","type":"journal-article","created":{"date-parts":[[2013,8,28]],"date-time":"2013-08-28T11:27:00Z","timestamp":1377689220000},"source":"Crossref","is-referenced-by-count":4,"title":["Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niter\u00f3i, Brazil"],"prefix":"10.2478","volume":"58","author":[{"given":"Israel","family":"Junior","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mauricio","family":"Vericimo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana","family":"Cardoso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Clemente","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elmiro","family":"Nascimento","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerlinde","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref71","first-page":"97","article-title":"Endoscopic imaging of the first clinical case of anisakidosis in Brazil","volume":"8","author":"Cruz","year":"2010","journal-title":"Scientia Parasitologica"},{"key":"ref181","doi-asserted-by":"publisher","first-page":"1903","DOI":"10.4067\/S0717-77122007000200005","article-title":"Anisakidae parasitos de congro - rosa Genypterus brasiliensis Regan comercializados no estado do Rio de Janeiro , Brasil de interesse na sa\u00fade p\u00fablica http dx doi org","volume":"19","author":"Knoff","year":"2007","journal-title":"Parasitolog\u00eda Latinoamericana"},{"key":"ref271","doi-asserted-by":"publisher","first-page":"1845","DOI":"10.1590\/S1676-06032008000200010","article-title":"Gastric lesions associated with the presence of Anisakis spp in Cetaceans stranded on the coast of Ceara Biota Neotropica http dx doi org","volume":"28","author":"Motta","year":"2008","journal-title":"Dujardin"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1007\/s00436-008-1105-2.http:\/\/dx.doi.org\/10.1007\/s00436-008-1105-2","article-title":"Isolation of Ani s an excretory - secretory and highly heat - resistant allergen useful for the diagnosis of Anisakis larvae sensitization","volume":"5","author":"Caballero","year":"2008","journal-title":"Parasitology Research"},{"key":"ref81","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1007\/s00403-012-1206-8","article-title":"Ani s s recombinant allergens are able to differentiate distinct Anisakis simplex - associated allergic clinical disorders","volume":"9","author":"Cuellar","year":"2012","journal-title":"Ani Archives of Dermatological Research"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1128\/CMR.00012-07.http:\/\/dx.doi.org\/10.1128\/CMR.00012-07","article-title":"Anisakis simplex : from obscure infectious worm to inducer of immune hypersensitivity pii","volume":"4","author":"Audicana","year":"2008","journal-title":"Clinical Microbiology Reviews"},{"key":"ref221","first-page":"193","article-title":"Protein Measurement with the Folin Phenol Reagent","volume":"23","author":"Lowry","year":"1951","journal-title":"Journal of Biological Chemistry"},{"key":"ref261","first-page":"142","article-title":"Incidence of anaphylaxis and subtypes of anaphylaxis in a general hospital emergency department","volume":"27","author":"Moro Moro","year":"2011","journal-title":"Journal of Investigational Allergology and Clinical Immunology"},{"key":"ref141","first-page":"175","article-title":"Anisakidae nematodes and Trypanorhyncha cestodes of hygienic importance infecting the king mackerel Scomberomorus cavalla Osteichthyes in Brazil","volume":"15","author":"Dias","year":"2011","journal-title":"Veterinary Parasitology"},{"key":"ref331","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1016\/j.exppara.2007.07.002.http:\/\/dx.doi.org\/10.1016\/j.exppara.2007.07.002","article-title":"Anisakis simplex : the high prevalence in Madrid and its relation with fish consumption pii","volume":"34","author":"Puente","year":"2008","journal-title":"Experimental Parasitology"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/S1471-4922(01)02152-3","article-title":"Anisakis simplex : dangerous - dead and alive pii http dx doi org","volume":"3","author":"Audicana","year":"2002","journal-title":"Trends Parasitology"},{"key":"ref211","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T http dx doi org","volume":"22","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"ref371","doi-asserted-by":"publisher","first-page":"224","DOI":"10.1111\/j.1365-4632.2006.03090.x","article-title":"Anisakis simplex and cross - reacting antigens pii","volume":"38","author":"Weiler","year":"2007","journal-title":"International Journal of Dermatology"},{"key":"ref351","doi-asserted-by":"publisher","first-page":"4330","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polycrylamide gels to nitrocellulose sheets : Procedures and some applications Proceedings of the National Academy of http dx doi org","volume":"36","author":"Towbin","year":"1979","journal-title":"Sciences"},{"key":"ref231","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1111\/j.1095-8649.2009.02515.x.http:\/\/dx.doi.org\/10.1111\/j.1095-8649.2009.02515.x","article-title":"Metazoan parasites as biological tags for stock discrimination of whitemouth croaker Micropogonias furnieri","volume":"24","author":"Luque","year":"2010","journal-title":"Journal of Fish Biology"},{"key":"ref161","doi-asserted-by":"publisher","first-page":"1550","DOI":"10.1016\/j.foodcont.2010.03.019","article-title":"Occurrence and infection dynamics of anisakid larvae in Scomber scombrus Trachurus trachurus pilchardus and Engraulis encrasicolus from Tarragona NE http dx doi org","volume":"17","author":"Guti\u00e9rrez","year":"2010","journal-title":"Food Control"},{"key":"ref151","doi-asserted-by":"publisher","first-page":"554","DOI":"10.1016\/S0091-6749(98)70364-1","article-title":"Allergenic crossreactivity between third stage larvae of Hysterothylacium aduncum and Anisakis simplex pii http dx doi org","volume":"16","author":"Fernandez","year":"1998","journal-title":"Journal of Allergy and Clinical Immunology"},{"key":"ref91","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1046\/j.1365-2133.1998.02507.x","article-title":"Gastric anisakiasis : an underestimated cause of acute urticaria and angiooedema http dx doi org","volume":"10","author":"Daschner","year":"1998","journal-title":"British Journal of Dermatology"},{"key":"ref241","first-page":"353","article-title":"Use of fish as intermediate hosts by helminth parasites : A comparative analysis","volume":"25","author":"Luque","year":"2004","journal-title":"Acta Parasitologica"},{"key":"ref381","first-page":"199","article-title":"Yp - Antibodies in Guinea - Pigs Infected with the Cattle Lungworm","volume":"39","author":"Wilson","year":"1966","journal-title":"Immunology"},{"key":"ref361","first-page":"108","article-title":"Anisakis allergy : an update Revue Fran\u00e7ise d","volume":"37","author":"Valls","year":"2005","journal-title":"Allergologie"},{"key":"ref311","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/0002-9343(75)90328-9","article-title":"Intestinal anisakiasis First case report from North America","volume":"32","author":"Pinkus","year":"1975","journal-title":"American Journal of Medicine"},{"key":"ref111","first-page":"0301","article-title":"Different clinical presentation of Anisakis simplex associated urticaria is dependent on the frequency of raw fish intake pii","volume":"12","author":"Daschner","year":"2010","journal-title":"Allergologia et Immunopathologia"},{"key":"ref251","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1590\/S1516-89132006000100017","article-title":"Helminths of the marine tucuxi Sotalia fluviatilis in northern Rio de Janeiro State","volume":"26","author":"Melo","year":"2006","journal-title":"Brazilian Archives of Biology and Technology"},{"key":"ref131","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.actatropica.2005.11.007.http:\/\/dx.doi.org\/10.1016\/j.actatropica.2005.11.007","article-title":"Sensitization to Anisakis simplex s l in a healthy population pii","volume":"14","author":"Del Rey Moreno","year":"2006","journal-title":"Acta Tropica"},{"key":"ref121","doi-asserted-by":"publisher","first-page":"977","DOI":"10.1016\/S0091-6749(96)80073-X","article-title":"Laboratory determinations in Anisakis simplex allergy pii http dx doi org","volume":"13","author":"Del Pozo","year":"1996","journal-title":"Journal of Allergy and Clinical Immunology"},{"key":"ref101","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.pt.2011.10.001.http:\/\/dx.doi.org\/10.1016\/j.pt.2011.10.001","article-title":"The Anisakis allergy debate : does an evolutionary approach help pii","volume":"11","author":"Daschner","year":"2012","journal-title":"Trends Parasitology"},{"key":"ref201","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1016\/j.parint.2011.01.004.http:\/\/dx.doi.org\/10.1016\/j.parint.2011.01.004","article-title":"Identification of novel three allergens from Anisakis simplex by chemiluminescent immunoscreening of an expression cDNA library pii","volume":"21","author":"Kobayashi","year":"2011","journal-title":"Parasitology International"},{"key":"ref321","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1089\/fpd.2009.0484.http:\/\/dx.doi.org\/10.1089\/fpd.2009.0484","article-title":"Anisakiasis , an underestimated infection : effect on intestinal permeability of Anisakis simplex - sensitized patients and","volume":"33","author":"Polimeno","year":"2010","journal-title":"Foodborne Pathogens Disease"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1111\/j.1365-2249.2009.03919.x.http:\/\/dx.doi.org\/10.1111\/j.1365-2249.2009.03919.x","article-title":"The Anisakis simplex Ani s major allergen as an indicator of true Anisakis infections and pii","volume":"2","author":"Anadon","year":"2009","journal-title":"Clinical Experimental Immunology"},{"key":"ref01","first-page":"496","article-title":"Diagnosing human anisakiasis : recombinant Ani s s allergens versus the UniCAP fluorescence enzyme immunoassay and pii","volume":"1","author":"Anadon","year":"2010","journal-title":"Ani Clinical Vaccine Immunology"},{"key":"ref171","doi-asserted-by":"publisher","first-page":"139","DOI":"10.3347\/kjp.2011.49.2.139.http:\/\/dx.doi.org\/10.3347\/kjp.2011.49.2.139","article-title":"Seroprevalence of antibodies against Anisakis simplex larvae among health - examined residents in three hospitals of southern parts of Korea","volume":"18","author":"Kim","year":"2011","journal-title":"Korean Journal of Parasitology"},{"key":"ref291","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.exppara.2011.09.008.http:\/\/dx.doi.org\/10.1016\/j.exppara.2011.09.008","article-title":"Identification of a kDa excretory secretory protein in Anisakis simplex pii","volume":"30","author":"Park","year":"2012","journal-title":"Experimental Parasitology"}],"container-title":["Acta Parasitologica"],"original-title":[],"link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.2478\/s11686-013-0157-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.2478\/s11686-013-0157-3\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.2478\/s11686-013-0157-3","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,4]],"date-time":"2021-03-04T05:21:11Z","timestamp":1614835271000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.2478\/s11686-013-0157-3\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,1]]},"references-count":33,"journal-issue":{"issue":"3"},"URL":"https:\/\/doi.org\/10.2478\/s11686-013-0157-3","relation":{},"ISSN":["1896-1851","1230-2821"],"issn-type":[{"type":"electronic","value":"1896-1851"},{"type":"print","value":"1230-2821"}],"subject":[],"published":{"date-parts":[[2013,1,1]]}}}