{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T20:25:08Z","timestamp":1787862308113,"version":"build-2784847793"},"reference-count":26,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, two pairs of oligonucleotide primers were designed according to the nucleotide sequence of the internal transcribed spacers (ITSs) of Babesia bigemina and B. bovis isolates from China. The primers were used in a multiplex PCR to detect parasite DNA in blood samples from cattle. There was no cross reactions with B. ovata, B. major, B. sp. Kashi, Theileria annulata, T. sergenti, T. sinensis or normal bovine DNA. The sensitivity of multiplex PCR assay was 1 pg and 10 pg DNA for B. bigemina and B. bovis, respectively. A total of 260 field blood samples collected from cattle in five provinces of China were analyzed by multiplex PCR and light microscopy. PCR testing revealed that 7.3% (19\/260) and 5.8% (15\/260) of cattle were positive for B. bigemina and B. bovis and 1.2% (3\/260) of cattle were co-infected with B. bigemina and B. bovis. Using light microscopy, 2.3% (6\/260) and 1.5% (4\/260) of cattle were infected by B. bigemina and B. bovis, respectively, and no co-infection was found. The results showed that the multiplex PCR developed in the present study could be an alternative diagnostic tool for the detection of B. bovis and B. bigemina infection in cattle.<\/jats:p>","DOI":"10.2478\/s11686-014-0222-6","type":"journal-article","created":{"date-parts":[[2014,2,25]],"date-time":"2014-02-25T05:22:00Z","timestamp":1393305720000},"source":"Crossref","is-referenced-by-count":13,"title":["A PCR method targeting internal transcribed spacers: the simultaneous detection of Babesia bigemina and Babesia bovis in cattle"],"prefix":"10.2478","volume":"59","author":[{"given":"Junlong","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiquan","family":"Guan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aihong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youquan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Yin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianxun","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"ref161","first-page":"5","article-title":"Discrimination of Babesia major and Babesia ovata based on ITS ITS region sequences of rRNA gene","volume":"17","author":"Liu","year":"2008","journal-title":"Parasitology Research"},{"key":"ref91","first-page":"46","article-title":"A report of buffalo babesiosis of Animals Husbandry In Chinese","volume":"10","author":"Fu","year":"1997","journal-title":"Guangxi Journal Veterinary Medicine"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1128\/JCM.42.1.99-105.2004","article-title":"Infection with a proposed new subspecies of Babesia canis Babesia canis subsp present in domestic cat http dx doi org","volume":"5","author":"Baneth","year":"2004","journal-title":"Journal of Clinical Microbiology"},{"key":"ref131","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/S1383-5769(03)00028-X","article-title":"A multiplex polymerase chain reaction for the differential diagnosis of Plasmodium falciparum and Plasmodium vivax http dx doi org","volume":"14","author":"Kho","year":"2003","journal-title":"Parasitology International"},{"key":"ref231","article-title":"Diagnosis and therapy of bovine babesiosis Husbandry and in Chinese","volume":"24","author":"Qin","year":"2005","journal-title":"Today Animal Veterinary Medicine"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/S0304-4017(01)00464-2","article-title":"Bovine piroplasms in Minorca ( Balearic Islands : a comparison of PCR - 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