{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T16:32:32Z","timestamp":1793464352717,"version":"4.5.0"},"reference-count":8,"publisher":"Walter de Gruyter GmbH","issue":"2","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Classification and identification of muscle-parasitizing didymozoids found in marine fish is difficult because of their novel parasitism and morphology. Recent sequence analysis has helped, but only seven sequences are available. Therefore, the usefulness of molecular methods for differentiation of muscle-parasitizing didymozoids, as well as genetic differences between the muscle and the other site-parasitizing didymozoids are quite unclear. In the present study, six unidentified didymozoid isolates from the trunk muscles of four marine fish species (Diagramma pictum, Plectorhinchus cinctus, Pagrus major and Cypselurus heterurus) were examined genetically using sequence analysis (18S rDNA, 28S rDNA, ITS-2 and coxI). All isolates were placed phylogenetically as a lineage independent of other site-parasitizing didymozoids at 18S rDNA, ITS-2 and coxI. They were grouped into three distinct lineages. The present and the previous unidentified or identified didymozoids from trunk muscles were found to differ clearly for every host species by sequence analysis, suggesting that muscle-parasitizing didymozoids are host-specific. This report is the first describing the molecular characteristics of muscle-parasitizing didymozoids by sequence analysis targeting the nuclear and mitochondrial DNA loci, which is proposed as a superior method for didymozoid differentiation.<\/jats:p>","DOI":"10.2478\/s11686-014-0234-2","type":"journal-article","created":{"date-parts":[[2014,5,14]],"date-time":"2014-05-14T04:51:46Z","timestamp":1400043106000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Molecular characterization of muscle-parasitizing didymozoids in marine fishes"],"prefix":"10.2478","volume":"59","author":[{"given":"Niichiro","family":"Abe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuru","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomofumi","family":"Maehara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"374","reference":[{"key":"ref41","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1017\/S0031182009991703.http:\/\/dx.doi.org\/10.1017\/S0031182009991703","article-title":"Multilocus phylogenetic analyses reveal that habitat selection drives the speciation of Didymozoidae parasitizing Pacific and Atlantic bluefin tunas","volume":"137","author":"Mladineo","year":"2010","journal-title":"Parasitology"},{"key":"ref91","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1016\/S0020-7519(03)00049-3.http:\/\/dx.doi.org\/10.1016\/S0020-7519(03)00049-3","article-title":"Phylogeny and classification of the Digenea for","volume":"33","author":"Olson","year":"2003","journal-title":"International Journal Parasitology"},{"key":"ref01","doi-asserted-by":"publisher","first-page":"227","DOI":"10.2478\/s11686-011-0029-7.http:\/\/dx.doi.org\/10.2478\/s11686-011-0029-7","article-title":"Molecular and morphological identification of helminthes found in Japanese smelt Hypomesus transpacificus nipponensis with notes on new host records of Eustrongylides ignotus and Raphidascaris gigi","volume":"1","author":"Abe","year":"2011","journal-title":"Acta Parasitologica"},{"key":"ref121","first-page":"97","article-title":"Molecular identification of animal foreign substances in foods and food products : Identification of a parasite in the muscle of Crescent sweetlips Plectorhinchus cinctus Report of Hokkaido Institute of Public","volume":"57","author":"Yagi","year":"2007","journal-title":"Health"},{"key":"ref61","first-page":"175","article-title":"A trematodes Gonapodasmius okushimai ISHII found in the muscle of red sea bream Pagrosomus major http dx doi org","volume":"8","author":"Nakajima","year":"1974","journal-title":"Fish Pathology"},{"key":"ref11","first-page":"29","article-title":"Identification of parasites found in fresh fish by morphological and sequencing analyses Annual Report of Osaka City Institution Public and","volume":"73","author":"Abe","year":"2011","journal-title":"Health Environmental Science"},{"key":"ref31","first-page":"279","article-title":"Studies of the family Didymozooidae of","volume":"6","author":"Ishii","year":"1935","journal-title":"Japanese Journal Zoology"},{"key":"ref101","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/j.icesjms.2005.08.010.http:\/\/dx.doi.org\/10.1016\/j.icesjms.2005.08.010","article-title":"Host - parasite interaction of a muscle - infecting didymozoid in the Atlantic mackerel Scomber scombrus L of","volume":"63","author":"Pascual","year":"2006","journal-title":"ICES Journal Marine Science"}],"container-title":["Acta Parasitologica"],"original-title":[],"link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.2478\/s11686-014-0234-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.2478\/s11686-014-0234-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.2478\/s11686-014-0234-2","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,4]],"date-time":"2021-03-04T05:21:33Z","timestamp":1614835293000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.2478\/s11686-014-0234-2\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,1,1]]},"references-count":8,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.2478\/s11686-014-0234-2","relation":{},"ISSN":["1896-1851"],"issn-type":[{"value":"1896-1851","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,1,1]]}}}