{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T14:08:33Z","timestamp":1770905313872,"version":"3.50.1"},"reference-count":35,"publisher":"Walter de Gruyter GmbH","issue":"2","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Echinococcus multilocularis is one of the most pathogenic zoonotic parasites in the temperate and arctic region of the Northern Hemisphere. For estimating the potential risk of human infection in endemic areas, reliable antemortem methods are needed to detect the parasite in carnivore definitive hosts. The sensitivity of routine flotation techniques for detection of E. multilocularis eggs was found to be low (3\u201333%) depending on the flotation solution used (specific gravities = 1.3\u20131.4). An improved faecal flotation followed by a species-specific PCR is described with a sensitivity of 74% (95% CI = 62\u201384%) and a specificity of 100% (95% CI = 94\u2013100%). These parameters are similar to those of the intestinal scraping technique (sensitivity = 78%, specificity = 100%). The sensitivity of the improved flotation was significantly higher (P &lt; 0.0001) than that of routine flotation techniques. The costs of the method are similar or lower than those of other antemortem diagnostic methods. Based on these data, the method is suitable for surveys of domesticated and wild carnivores.<\/jats:p>","DOI":"10.2478\/s11686-014-0248-9","type":"journal-article","created":{"date-parts":[[2014,5,14]],"date-time":"2014-05-14T04:51:46Z","timestamp":1400043106000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Evaluation of faecal flotation methods followed by species-specific PCR for detection of Echinococcus multilocularis in the definitive hosts"],"prefix":"10.2478","volume":"59","author":[{"given":"Zolt\u00e1n","family":"Sz\u00e9ll","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zsuzsanna","family":"Sr\u00e9ter-Lancz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tam\u00e1s","family":"Sr\u00e9ter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref141","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.ijpara.2005.08.012","article-title":"Reproductive potential of Echinococcus multilocularis in experimentally infected foxes dogs raccoon dogs and cats http dx org","volume":"15","author":"Kapel","year":"2006","journal-title":"International Journal for Parasitology"},{"key":"ref311","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/S0304-4017(98)00263-5","article-title":"der Detection of Echinococcus multilocularis in foxes in The Netherlands http dx org","volume":"32","author":"van","year":"1999","journal-title":"Veterinary Parasitology"},{"key":"ref241","doi-asserted-by":"publisher","first-page":"1344","DOI":"10.3201\/eid1007.031027","article-title":"Echinococcus multilocularis in Northern Hungary http dx org","volume":"25","author":"Sr\u00e9ter","year":"2004","journal-title":"Emerging Infectious Diseases"},{"key":"ref81","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1128\/JCM.36.7.1871-1876.1998","article-title":"von Nickisch Detection of Echinococcus multilocularis in the definitive host : coprodiagnosis by PCR as an alternative to necropsy","volume":"9","author":"Dinkel","year":"1998","journal-title":"Journal of Clinical Microbiology"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"68","DOI":"10.2307\/3281005","article-title":"Determination of the specific gravity of certain helminth eggs using sucrose density gradient centrifugation http dx org","volume":"5","author":"David","year":"1982","journal-title":"Journal of Parasitology"},{"key":"ref51","first-page":"245","article-title":"Diagnosis of the Echinococcus multilocularis infection in final hosts","volume":"6","author":"Deplazes","year":"1996","journal-title":"Applied Parasitology"},{"key":"ref181","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1079\/JOH200038","article-title":"a Echinococcus multilocularis in carnivores from the Klatovy district of the Czech Republic http dx org","volume":"19","author":"Mart\u00ednek","year":"2001","journal-title":"Journal of Helminthology"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"2059","DOI":"10.3201\/eid1812.120219","article-title":"Westward spread of Echinococcus multilocularis in foxes http dx org","volume":"4","author":"Combes","year":"2012","journal-title":"Emerging Infectious Diseases"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1016\/j.vetpar.2010.08.023","article-title":"Spatial distribution and genetic diversity of Echinococcus multilocularis in Hungary http dx org","volume":"3","author":"Casulli","year":"2010","journal-title":"Veterinary Parasitology"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"1079","DOI":"10.1016\/j.ijpara.2005.04.005","article-title":"Echinococcus multilocularis in red foxes ( Vulpes vulpes ) of the Italian Alpine region : is there a focus of autochthonous transmission http dx org","volume":"2","author":"Casulli","year":"2005","journal-title":"International Journal for Parasitology"},{"key":"ref271","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1645\/GE-3057.1","article-title":"Echinococcus multilocularis identified in Michigan with additional records from Ohio http dx org","volume":"28","author":"Storandt","year":"2012","journal-title":"Journal of Parasitology"},{"key":"ref261","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.vetpar.2011.01.065","article-title":"Bayesian space - 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