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In the present study, we established short- and long-term in vitro cultures of G. duodenalis trophozoites originating from red deer and Thomson\u2019s gazelle (artiodactyls) and genetically characterised the isolates by their glutamate dehydrogenase and triose phosphate isomerase gene sequences. The G. duodenalis isolates from red deer and the gazelle represented assemblages A (AIII sub-assemblage) and B. In conclusion, G. duodenalis assemblages and sub-assemblages can be associated with differences in growth rate in vitro cultures.<\/jats:p>","DOI":"10.2478\/s11686-014-0279-2","type":"journal-article","created":{"date-parts":[[2014,9,19]],"date-time":"2014-09-19T00:25:25Z","timestamp":1411086325000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Axenic in vitro culture and molecular characterization of Giardia duodenalis from red deer (Cervus elaphus) and Thomson\u2019s gazelle (Gazella thomsonii)"],"prefix":"10.2478","volume":"59","author":[{"given":"Piotr","family":"Solarczyk","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Majewska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"S\u0142odkowicz-Kowalska","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"374","reference":[{"key":"ref71","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1016\/j.molbiopara.2008.04.006","article-title":"Molecular epidemiology of giardiasis http dx org","volume":"8","author":"Cacci\u00f2","year":"2008","journal-title":"Molecular and Biochemical Parasitology"},{"key":"ref211","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1016\/j.vetpar.2011.03.049.http:\/\/dx.doi.org\/10.1016\/j.vetpar.2011.03.049","article-title":"de Genotyping of potentially zoonotic Giardia duodenalis from exotic and wild animals kept in captivity in Brazil","volume":"22","author":"Soares","year":"2011","journal-title":"Veterinary Parasitology"},{"key":"ref01","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1017\/S0031182000074540","article-title":"Selection of specific genotypes of Giardia intestinalis by growth in vitro and in vivo http dx org","volume":"1","author":"Andrews","year":"1992","journal-title":"Parasitology"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"1523","DOI":"10.1016\/j.ijpara.2008.04.008.http:\/\/dx.doi.org\/10.1016\/j.ijpara.2008.04.008","article-title":"Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B for","volume":"7","author":"Cacci\u00f2","year":"2008","journal-title":"International Journal Parasitology"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/0020-7519(92)90101-P","article-title":"Comparative studies on the growth dynamics of two genetically distinct isolates of Giardia duodenalis in vitro for http dx org","volume":"5","author":"Binz","year":"1992","journal-title":"International Journal Parasitology"},{"key":"ref261","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1017\/S0031182010001459.http:\/\/dx.doi.org\/10.1017\/S0031182010001459","article-title":"Risk of human infection with Giardia duodenalis from cats in Japan and genotyping of the isolates to assess the route of infection in cats","volume":"27","author":"Suzuki","year":"2011","journal-title":"Parasitology"},{"key":"ref171","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/0014-4894(76)90016-3","article-title":"Giardia lamblia : isolation and axenic cultivation http dx org","volume":"18","author":"Meyer","year":"1976","journal-title":"Experimental Parasitology"},{"key":"ref231","doi-asserted-by":"publisher","first-page":"59","DOI":"10.14411\/fp.2012.032","article-title":"Multilocus genotyping of Giardia duodenalis isolates from red deer ( Cervus elaphus ) and roe deer ( Capreolus capreolus ) from Poland http dx org","volume":"24","author":"Solarczyk","year":"2012","journal-title":"Folia Parasitologica"},{"key":"ref131","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1017\/S0031182006001533","article-title":"Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark http dx org","volume":"14","author":"Langkjaer","year":"2007","journal-title":"Parasitology"},{"key":"ref191","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.meegid.2004.02.001","article-title":"Discrimination of all genotypes of Giardia duodenalis at the glutamate dehydrogenase locus using PCR - 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