{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T10:40:10Z","timestamp":1717584010479},"reference-count":0,"publisher":"Walter de Gruyter GmbH","issue":"1","license":[{"start":{"date-parts":[[2011,12,25]],"date-time":"2011-12-25T00:00:00Z","timestamp":1324771200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study describes the development of a micropropagation protocol for Pinguicula vulgaris using cultures initiated from in vitro produced seedlings. P. vulgaris is a carnivorous plant with a northern, disjunctly circumpolar distribution and specific habitat requirements, and is hence becoming increasingly rare. Shoot proliferation was significantly influenced by Murashige and Skoog (MS) macronutrient concentration, showing higher proliferation rates in 1\/4MS, but was not affected by the addition of 0.1 mg\/L 6-benzyladenine (BA) or zeatin (Zea). The best medium for propagating P. vulgaris was plant growth regulator (PGR) free \u00bcMS. An average of 7.62 new shoots per initial explant could be obtained after 8 weeks of culture, of which over 79% produced roots during proliferation. Moreover, rooting percentages of 100% were obtained for the initial explants in all the tested media, including media without PGRs. The plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development.<\/jats:p>","DOI":"10.2478\/s11535-011-0103-z","type":"journal-article","created":{"date-parts":[[2011,12,24]],"date-time":"2011-12-24T06:56:45Z","timestamp":1324709805000},"page":"48-53","source":"Crossref","is-referenced-by-count":2,"title":["In vitro plantlet production of the endangered Pinguicula vulgaris"],"prefix":"10.2478","volume":"7","author":[{"given":"Tom\u00e1s","family":"Grevenstuk","sequence":"first","affiliation":[{"name":"Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB\/CGB), Faculty of Sciences and Technology, University of Algarve, 8005-139, Faro, Portugal"}]},{"given":"Anabela","family":"Romano","sequence":"additional","affiliation":[{"name":"Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB\/CGB), Faculty of Sciences and Technology, University of Algarve, 8005-139, Faro, Portugal"}]}],"member":"374","published-online":{"date-parts":[[2011,12,25]]},"container-title":["Open Life Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.degruyter.com\/view\/journals\/biol\/7\/1\/article-p48.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.2478\/s11535-011-0103-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.2478\/s11535-011-0103-z\/xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.2478\/s11535-011-0103-z\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,5]],"date-time":"2024-06-05T09:52:08Z","timestamp":1717581128000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.2478\/s11535-011-0103-z\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12,25]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2011,12,25]]},"published-print":{"date-parts":[[2012,2,1]]}},"alternative-id":["10.2478\/s11535-011-0103-z"],"URL":"https:\/\/doi.org\/10.2478\/s11535-011-0103-z","relation":{},"ISSN":["2391-5412"],"issn-type":[{"value":"2391-5412","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12,25]]}}}