{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T19:06:51Z","timestamp":1758395211782,"version":"3.37.3"},"reference-count":22,"publisher":"Walter de Gruyter GmbH","issue":"4","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Maritrema portucalensis sp. nov. (Digenea, Microphallidae) from Aveiro estuary, northern Portugal, is described on the basis of excysted metacercariae obtained from the gill lamellae of the shore crab Carcinus maenas (L.). M. portucalensis sp. nov. most closely resembles Maritrema subdolum J\u00e4gerski\u00f6ld, 1909, a species that also frequently uses C. maenas as second intermediate host. The new species differs from M. subdolum in having a narrower body at the level of testes and a smaller ovary and testes. Also, the digestive caeca presented various folds along their length, thickening in the terminal region. Moreover, the body surface was fully covered by tegumental spines provided with several teeth. To complement our morphological characterization and to identify M. portucalensis metacercariae, the ITS1 rDNA region of the cysts isolated from C. maenas was sequenced and compared with the corresponding available sequences of digenean trematodes belonging to the family Microphallidae. Alignments revealed 28 base-pair differences between the query-ITS1 and that of M. subdolum, and more importantly a 100% similarity with the sequence of Microphallidae sp. no. 15 cercaria from the snail Hydrobia ulvae. The last observation allowed us to establish an unequivocal association between cercaria known as Microphallidae sp. no. 15, and the metacercaria of M. portucalensis sp. nov. characterized in this work, contributing decisively to the clarification of its life cycle.<\/jats:p>","DOI":"10.2478\/s11686-011-0068-0","type":"journal-article","created":{"date-parts":[[2011,12,8]],"date-time":"2011-12-08T21:47:00Z","timestamp":1323380820000},"source":"Crossref","is-referenced-by-count":9,"title":["Description of Maritrema portucalensis sp. nov. (Digenea, Microphallidae) parasite of Carcinus maenas (Crustacea, Decapoda) from Aveiro estuary, Northern Portugal"],"prefix":"10.2478","volume":"56","author":[{"given":"Susana","family":"Pina","sequence":"first","affiliation":[]},{"given":"Fernanda","family":"Russell-Pinto","sequence":"additional","affiliation":[]},{"given":"Pedro","family":"Rodrigues","sequence":"additional","affiliation":[]}],"member":"374","reference":[{"key":"ref151","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1016\/0020-7519(96)82612-9.http:\/\/dx.doi.org\/10.1016\/0020-7519(96)82612-9","article-title":"Molecular genetic approaches to parasite identification : their value in diagnostic parasitology and systematics for","volume":"16","author":"McManus","year":"1996","journal-title":"International Journal Parasitology"},{"key":"ref91","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1007\/s00436-004-1136-2.http:\/\/dx.doi.org\/10.1007\/s00436-004-1136-2","article-title":"Use of ITS rDNA for discriminating of larval stages of two microphallid species using Hydrobia ulvae and Corophium volutator as intermediate hosts","volume":"10","author":"Hust","year":"2004","journal-title":"Pallas Parasitology Research"},{"key":"ref221","first-page":"979","article-title":"Systema Helminthum The digenetic trematodes of vertebrates Part Interscience Publishers New","volume":"23","author":"Yamaguti","year":"1958","journal-title":"York"},{"key":"ref141","first-page":"295","article-title":"Encapsulaci\u00f3n de dos metacercarias en Cyrtograpsus angulatus y Palaemonetes argentinus de","volume":"15","author":"Martorelli","year":"1990","journal-title":"Revista Biologia Tropical"},{"key":"ref51","first-page":"1","article-title":"Organization of parthenogenetic and hermaphroditic generations of trematodes In Fried and The biology and evolution of trematodes Kluwer Academic Publishers , The","volume":"6","author":"Galaktionov","year":"2003","journal-title":"Netherlands"},{"key":"ref121","doi-asserted-by":"publisher","first-page":"1403","DOI":"10.1016\/S0020-7519(98)00117-9.http:\/\/dx.doi.org\/10.1016\/S0020-7519(98)00117-9","article-title":"Use of the ITS rDNA for elucidation of some life - 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