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Specific haemagglutinating activity (SHA) decreased by 94 % for the extract and cMoL and by 50 % for the fraction in the presence of humic acid. Precipitation bands were observed in the diffusion gel. Both results suggested humic acid-cMoL binding. Carbohydrates, potassium, and calcium ions and pH affected the SHA of cMoL. As an example of application, cMoL was immobilised on a column packed with sepharose receiving 20 mg mL\u22121 of carbon humic acid solution, 30 mg of humic acid per gram of support was removed. This result suggested that protein preparations might be used in water treatment to remove humic acids.<\/jats:p>","DOI":"10.2478\/s11696-011-0025-2","type":"journal-article","created":{"date-parts":[[2011,3,25]],"date-time":"2011-03-25T17:22:30Z","timestamp":1301073750000},"source":"Crossref","is-referenced-by-count":5,"title":["Interaction of Moringa oleifera seed lectin with humic acid"],"prefix":"10.1007","volume":"65","author":[{"given":"Andr\u00e9a","family":"Santos","sequence":"first","affiliation":[]},{"given":"Maria","family":"Carneiro-da-Cunha","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9","family":"Teixeira","sequence":"additional","affiliation":[]},{"given":"Patr\u00edcia","family":"Paiva","sequence":"additional","affiliation":[]},{"given":"Luana","family":"Coelho","sequence":"additional","affiliation":[]},{"given":"Regina","family":"Nogueira","sequence":"additional","affiliation":[]}],"member":"297","reference":[{"key":"ref361","doi-asserted-by":"publisher","first-page":"3621","DOI":"10.1016\/j.watres.2006.05.044.http:\/\/dx.doi.org\/10.1016\/j.watres.2006.05.044","article-title":"Enhanced coagulation in a typical North - China water treatment plant","volume":"37","author":"Yan","year":"2006","journal-title":"Water Research"},{"key":"ref61","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1016\/S0968-0004(97)01146-8.http:\/\/dx.doi.org\/10.1016\/S0968-0004(97)01146-8","article-title":"Lectin - carbohydrate interactions : different folds , common recognition principles in","volume":"7","author":"Elgavish","year":"1997","journal-title":"Trends Biochemical Sciences"},{"key":"ref181","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/j.biologicals.2003.12.001.http:\/\/dx.doi.org\/10.1016\/j.biologicals.2003.12.001","article-title":"Mitogenic activity of Cratylia mollis lectin on human lymphocytes","volume":"19","author":"Maciel","year":"2004","journal-title":"Biologicals"},{"key":"ref241","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/j.microc.2008.02.001.http:\/\/dx.doi.org\/10.1016\/j.microc.2008.02.001","article-title":"Quartz Crystal Microbalance monitoring the real - time binding of lectin with carbohydrate with high and low molecular mass","volume":"25","author":"Pedroso","year":"2008","journal-title":"Microchemical Journal"},{"key":"ref281","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/S0960-8524(99)00018-8.http:\/\/dx.doi.org\/10.1016\/S0960-8524(99)00018-8","article-title":"Relationships between water - soluble carbohydrate and phenol fractions and the humification indices of different organic wastes during composting","volume":"29","author":"S\u00e1nchez","year":"1999","journal-title":"Bioresource Technology"},{"key":"ref31","first-page":"47","article-title":"Lectins carbohydrate recognition molecules : Are they toxic ? 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