{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T16:07:28Z","timestamp":1745338048376,"version":"3.37.3"},"reference-count":20,"publisher":"Springer Science and Business Media LLC","issue":"12","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The production of antimicrobial metabolites by Paenibacillus polymyxa RNC-D was assessed. Two process variables, glucose and inoculum concentrations, were evaluated at different levels (5\u201340 g L\u22121, and at \u03c6 r = 2.5\u20135.0 %, respectively), and their effects on biomass formation, minimal inhibitory concentration (MIC) against Escherichia coli, and surface tension reduction (STR) were studied. When the fermentation process was carried out under non-optimised conditions, the biomass, MIC, and STR achieved the following values: 0.6 g L\u22121, 1 g L\u22121, and 18.4 mN m\u22121, respectively. The optimum glucose (16 g L\u22121) and inoculum volume ratio (\u03c6 r = 5.0 %) were defined in order to maximise the biomass formation, with a low value of MIC and high STR of extract. The experiments carried out under optimal conditions showed the following values for the dependent variables: biomass concentration 2.05 g L\u22121, MIC 31.2 \u03bcg mL\u22121, and STR 10.7 mN m\u22121, which represented improvement of 241.7 %, 96.9 %, and 41.9 % for the responses of biomass, MIC, and STR, respectively. This is the first recorded study on the optimisation of culture conditions for the production of antimicrobial metabolites of P. polymyxa RNC-D, and constitutes an important step in the development of strategies to modulate the production of antimicrobial molecules by this microorganism at elevated levels.<\/jats:p>","DOI":"10.2478\/s11696-012-0242-3","type":"journal-article","created":{"date-parts":[[2012,9,12]],"date-time":"2012-09-12T03:15:03Z","timestamp":1347419703000},"source":"Crossref","is-referenced-by-count":8,"title":["Optimal glucose and inoculum concentrations for production of bioactive molecules by Paenibacillus polymyxa RNC-D"],"prefix":"10.1007","volume":"66","author":[{"given":"Nadja","family":"Serrano","sequence":"first","affiliation":[]},{"given":"Ligia","family":"Rodrigues","sequence":"additional","affiliation":[]},{"given":"Carlos","family":"Hokka","sequence":"additional","affiliation":[]},{"given":"Cristina","family":"Sousa","sequence":"additional","affiliation":[]},{"given":"Jos\u00e9","family":"Teixeira","sequence":"additional","affiliation":[]},{"given":"Solange","family":"Mussatto","sequence":"additional","affiliation":[]}],"member":"297","reference":[{"key":"ref191","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1021\/np030397v.http:\/\/dx.doi.org\/10.1021\/np030397v","article-title":"Natural products from endophytic microorganisms of","volume":"20","author":"Strobel","year":"2004","journal-title":"Journal Natural Products"},{"key":"ref91","doi-asserted-by":"publisher","first-page":"243","DOI":"10.7164\/antibiotics.25.243","article-title":"A new antibiotic , gatavalin Isolation and characterization The of","volume":"10","author":"Nakajima","year":"1972","journal-title":"Journal Antibiotics"},{"key":"ref201","doi-asserted-by":"publisher","first-page":"8245","DOI":"10.1016\/j.biortech.2008.03.039.http:\/\/dx.doi.org\/10.1016\/j.biortech.2008.03.039","article-title":"Medium optimization for antifungal active substances production from a newly isolated Paenibacillus sp using response surface methodology","volume":"21","author":"Wang","year":"2008","journal-title":"Bioresource Technology"},{"key":"ref01","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1016\/s0032-9592(03)00049-9.http:\/\/dx.doi.org\/10.1016\/S0032-9592(03)00049-9","article-title":"Optimization of process parameters for cephalosporin C production under solid state fermentation from Acremonium chrysogenum","volume":"1","author":"Adinarayana","year":"2003","journal-title":"Process Biochemistry"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"129","DOI":"10.7164\/antibiotics.49.129.http:\/\/dx.doi.org\/10.7164\/antibiotics.49.129","article-title":"Fusaricidin a new depsipeptide antibiotic produced by Bacillus polymyxa KT - Taxonomy fermentation isolation structure elucidation and biological activity The of","volume":"5","author":"Kajimura","year":"1996","journal-title":"Journal Antibiotics"},{"key":"ref61","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1128\/MMBR.41.2.449-474.1977","article-title":"The peptide antibiotics of Bacillus : chemistry biogenesis and possible functions","volume":"7","author":"Katz","year":"1977","journal-title":"Bacteriology Reviews"},{"key":"ref181","doi-asserted-by":"publisher","first-page":"1228","DOI":"10.1021\/jm00232a012.http:\/\/dx.doi.org\/10.1021\/jm00232a012","article-title":"Structure of the peptide antibiotic polypeptin of","volume":"19","author":"Sogn","year":"1976","journal-title":"Journal Medicinal Chemistry"},{"key":"ref51","doi-asserted-by":"publisher","first-page":"220","DOI":"10.7164\/antibiotics.50.220.http:\/\/dx.doi.org\/10.7164\/antibiotics.50.220","article-title":"Fusaricidins new depsipeptide antibiotics produced by Bacillus polymyxa KT Isolation structure elucidation and biological activity The of","volume":"6","author":"Kajimura","year":"1997","journal-title":"Journal Antibiotics"},{"key":"ref161","doi-asserted-by":"publisher","first-page":"996","DOI":"10.1017\/s0953756202006342.http:\/\/dx.doi.org\/10.1017\/S0953756202006342","article-title":"Endophytic fungi : a source of novel biologically active secondary metabolites","volume":"17","author":"Schulz","year":"2002","journal-title":"Mycological Research"},{"key":"ref21","doi-asserted-by":"publisher","first-page":"304","DOI":"10.7164\/antibiotics.25.304.http:\/\/dx.doi.org\/10.7164\/antibiotics.25.304","article-title":"a Jolipeptin a new peptide antibiotic Isolation physico - chemical and biological characteristics The of","volume":"3","author":"Ito","year":"1972","journal-title":"Journal Antibiotics"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.mycmed.2007.10.006.http:\/\/dx.doi.org\/10.1016\/j.mycmed.2007.10.006","article-title":"Impact of submerged culture conditions on growth and bioactive metabolite produced by endophyte Hypocrea spp isolated from Dillenia indica Linn in North - East India Journal de Mycologie M\u00e9dicale of","volume":"2","author":"Gogoi","year":"2008","journal-title":"Journal Medical Mycology"},{"key":"ref31","doi-asserted-by":"publisher","first-page":"309","DOI":"10.7164\/antibiotics.25.309.http:\/\/dx.doi.org\/10.7164\/antibiotics.25.309","article-title":"Jolipeptin a new peptide antibiotic II The mode of action of jolipeptin The of","volume":"4","author":"Ito","year":"1972","journal-title":"Journal Antibiotics"},{"key":"ref121","doi-asserted-by":"publisher","first-page":"294","DOI":"10.1590\/s1678-91992008000200007.http:\/\/dx.doi.org\/10.1590\/S1678-91992008000200007","article-title":"Antagonistic properties of some microorganisms isolated from Brazilian tropical savannah plants against Staphylococcus coagulase - positive strain of and","volume":"13","author":"Ratti","year":"2008","journal-title":"Journal Venomous Animals Toxins Including Tropical Diseases"},{"key":"ref111","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1111\/j.1574-6968.1995.tb07887.x.http:\/\/dx.doi.org\/10.1111\/j.1574-6968.1995.tb07887.x","article-title":"Gavaserin and saltavalin new peptide antibiotics produced by Bacillus polymyxa","volume":"12","author":"Pichard","year":"1995","journal-title":"FEMS Microbiology Letters"},{"key":"ref211","doi-asserted-by":"publisher","first-page":"141876","DOI":"10.1155\/2010\/141876","article-title":"Optimization of the fermentation process of Actinomycete strain Hhs of and","volume":"22","author":"Wang","year":"2010","journal-title":"Journal Biomedicine Biotechnology"},{"key":"ref151","doi-asserted-by":"publisher","first-page":"1639","DOI":"10.1021\/bp050219n.http:\/\/dx.doi.org\/10.1021\/bp050219n","article-title":"Variables that affect xylitol production from sugarcane bagasse hydrolysate in a zeolite fluidized bed reactor","volume":"16","author":"Santos","year":"2005","journal-title":"Biotechnology Progress"},{"key":"ref131","doi-asserted-by":"publisher","first-page":"1904","DOI":"10.1016\/j.biortech.2009.10.029.http:\/\/dx.doi.org\/10.1016\/j.biortech.2009.10.029","article-title":"Use of response surface methodology to evaluate the effect of metal ions Ca ) on production of antifungal compounds by Paenibacillus polymyxa","volume":"14","author":"Raza","year":"2010","journal-title":"Bioresource Technology"},{"key":"ref141","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1016\/j.colsurfb.2006.08.009.http:\/\/dx.doi.org\/10.1016\/j.colsurfb.2006.08.009","article-title":"van der Isolation and partial characterization of a biosurfactant produced by Streptococcus thermophilus A and :","volume":"15","author":"Rodrigues","year":"2006","journal-title":"Colloids Surfaces B Biointerfaces"},{"key":"ref81","doi-asserted-by":"publisher","first-page":"540","DOI":"10.1016\/j.procbio.2008.01.013.http:\/\/dx.doi.org\/10.1016\/j.procbio.2008.01.013","article-title":"Establishment of the optimum initial xylose concentration and nutritional supplementation of brewer s spent grain hydrolysate for xylitol production by Candida guilliermondii","volume":"9","author":"Mussatto","year":"2008","journal-title":"Process Biochemistry"},{"key":"ref71","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1007\/bf01569794","article-title":"Carbon catabolite regulation of rebeccamycin production in Saccharothrix aerocolonigenes of &","volume":"8","author":"Lam","year":"1989","journal-title":"Journal Industrial Microbiology Biotechnology"}],"container-title":["Chemical Papers"],"original-title":[],"link":[{"URL":"http:\/\/www.springerlink.com\/index\/pdf\/10.2478\/s11696-012-0242-3","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,3,4]],"date-time":"2021-03-04T18:48:43Z","timestamp":1614883723000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.degruyter.com\/document\/doi\/10.2478\/s11696-012-0242-3\/html"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1,1]]},"references-count":20,"journal-issue":{"issue":"12"},"URL":"https:\/\/doi.org\/10.2478\/s11696-012-0242-3","relation":{},"ISSN":["1336-9075"],"issn-type":[{"type":"electronic","value":"1336-9075"}],"subject":[],"published":{"date-parts":[[2012,1,1]]}}}