{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T08:07:32Z","timestamp":1781770052177,"version":"3.54.5"},"reference-count":38,"publisher":"Oceanside Publications Inc.","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["allergy asthma proc"],"published-print":{"date-parts":[[2023,3,1]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Diagnosis and management of eosinophilic esophagitis (EoE) occur <jats:italic>via<\/jats:italic> esophagogastroduodenoscopy with tissue biopsy.  <jats:bold>Objective:<\/jats:bold> We sought to determine if salivary microribonucleic acid (miRNA) levels could differentiate children with\n EoE, serving as a noninvasive biomarker.  <jats:bold>Methods:<\/jats:bold> Saliva was collected from children undergoing esophagogastroduodenoscopy (N = 291). miRNA analysis was conducted on 150 samples: EoE (n = 50), no pathologic alteration (n = 100). RNA was quantified with high throughput sequencing\n and aligned to build hg38 of the human genome using sequencing and alignment software. Quantile normalized levels of robustly expressed miRNAs (raw counts &gt; 10 in 10% of samples) were compared across EoE and non-EoE groups with Wilcoxon rank sum testing. miRNA biomarker candidates were\n selected based on variable importance projection (VIP) scoring with partial least squared discriminant analysis (VIP &gt; 1.5). Ability of these miRNAs to differentiate EoE status was assessed <jats:italic>via<\/jats:italic> logistic regression. Putative biologic targets for the miRNA candidates were determined\n in miRNA pathway analysis software.  <jats:bold>Results:<\/jats:bold> Of the 56 salivary miRNAs reliably detected, miR-205-5p displayed the largest difference between EoE and non-EoE groups (V = 1623, adjusted p = 0.029). Six miRNAs (miR-26b-5p, miR-27b-3p, Let-7i-5p, miR-142-5p, miR-30a-5p, miR-205-5p)\n displayed elevated VIP scores (&gt;1.5) and were able to differentiate EoE samples on logistic regression analysis with 70% sensitivity and 68% specificity. These six miRNAs demonstrated significant enrichment for gene targets involved in valine, leucine, and isoleucine biosynthesis (p = 0.0012),\n 2-oxycarboxylic acid metabolism (p = 0.043), and steroid hormone biosynthesis (p = 0.048).  <jats:bold>Conclusions:<\/jats:bold> Salivary miRNAs represent a noninvasive, biologically relevant measure that may aid disease monitoring of EoE.<\/jats:p>","DOI":"10.2500\/aap.2023.44.220102","type":"journal-article","created":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T04:30:44Z","timestamp":1678077044000},"page":"145-152","source":"Crossref","is-referenced-by-count":15,"title":["Salivary microRNAs in pediatric eosinophilic esophagitis"],"prefix":"10.2500","volume":"44","author":[{"given":"Pooja BM.","family":"Jhaveri","sequence":"first","affiliation":[{"name":"From the Division of Allergy & Immunology, Department of Pediatrics, Penn State Children's Hospital, Hershey, 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