{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T01:54:06Z","timestamp":1792547646536,"version":"4.5.0"},"reference-count":44,"publisher":"Oceanside Publications Inc.","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["allergy asthma proc"],"published-print":{"date-parts":[[2025,11,1]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Allergen immunotherapy (IT) in Europe is generally administered as monoallergen IT. However, in some patients and in other parts of the world, multiallergen IT is common practice. A key question is whether allergens in a mixture retain their allergenicity over\n time.\n                    <jats:bold>Objective:<\/jats:bold>\n                    To demonstrate if allergenicity, as measured by skin-prick testing (SPT), is maintained after 6 months in a sublingual immunotherapy (SLIT) maintenance vial with three allergen extracts in a single individual vaccine, including two pollens and Dermatophagoides.\n                    <jats:bold>Methods:<\/jats:bold>\n                    We prepared two maintenance SLIT vials that contained mixed Dermatophagoides species (2000 AU\/mL), ash tree and Bermuda grass pollens (1:50 w\/v), one 6 months before SPT and the second 0\u20102 weeks before SPT, and three fresh vials (0\u20102 weeks before SPT)\n for each individual allergen. Duplicate SPTs were conducted with all five vials, diluent and positive (histamine 1 mg\/mL) controls. Wheals were measured at 10 minutes (controls) and 20 minutes (allergen vaccines). The mean wheal diameter was calculated as the average of the longest and orthogonal\n diameters, i.e., (D\n                    <jats:sub>1<\/jats:sub>\n                    + D\n                    <jats:sub>2<\/jats:sub>\n                    )\/2. For each allergen extract, two replicate wheals were obtained per participant; their mean diameters were averaged to yield a patient-level mean wheal diameter. These patient-level means were then averaged across all the participants. The\n paired t-test was used to calculate statistically significant differences between the mean wheal diameters of the extracts, with significance at p &lt; 0.05.\n                    <jats:bold>Results:<\/jats:bold>\n                    The mean \u00b1 standard deviation wheal diameter produced by the 6-month-old and the fresh extracts\n were 8.7 \u00b1 3.3 mm and 8.4 \u00b1 2.87 mm, respectively. This difference was not significant. Moreover, the potency of both pollen extracts was conserved when mixed with house-dust mite extracts.\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    In our pilot study, allergenicity of tree and grass pollen\n allergens in an allergen mix with Dermatophagoides in a SLIT maintenance vial was maintained over 6 months. Replication in a larger population would consolidate our findings.\n                  <\/jats:p>","DOI":"10.2500\/aap.2025.46.250075","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T04:32:17Z","timestamp":1761712337000},"page":"e176-e182","source":"Crossref","is-referenced-by-count":3,"title":["<i>In-vivo<\/i>\n                    allergen extract potency is maintained in a sublingual immunotherapy maintenance vial of pollens and mites"],"prefix":"10.2500","volume":"46","author":[{"given":"D\u00e9sir\u00e9e","family":"Larenas-Linnemann","sequence":"first","affiliation":[{"name":"From the Center of Excellence in Asthma and Allergy Larenas, Hospital M\u00e9dica Sur, Mexico City, Mexico;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph","family":"Mosges","sequence":"additional","affiliation":[{"name":"Institute of Medical Statistics and Computational Biology, Faculty of Medicine, University of Cologne, Cologne, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1355","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"1572","DOI":"10.1016\/S0140-6736(00)78276-6","article-title":"Prophylactic inoculation against hay fever","volume":"177","author":"Noon","year":"1911","journal-title":"Lancet"},{"key":"B2","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1016\/S1081-1206(10)63136-5","article-title":"Allergen immunotherapy: therapeutic vaccines for allergic diseases. 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