{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T05:04:23Z","timestamp":1789535063026,"version":"build-2803163510"},"reference-count":25,"publisher":"Oceanside Publications Inc.","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["allergy asthma proc"],"published-print":{"date-parts":[[2026,9,1]]},"abstract":"\n Backgroud:<\/jats:bold>\n Nonspecific lipid transfer proteins (nsLTP) are major plant allergens associated with systemic allergic reactions. The major Cannabis allergen, Can s 3, has recently emerged as a potential contributor to nsLTP-mediated allergy and cross-reactivity. However, its\n role within the broader nsLTP sensitization network remains insufficiently characterized.\n Objective:<\/jats:bold>\n This study aimed to evaluate the prevalence of sensitization to Can s 3 and to characterize its co-sensitization patterns with food- and pollen-derived nsLTPs in a large\n molecular cohort.\n Methods:<\/jats:bold>\n A retrospective observational study, including 2409 patients referred for allergological evaluation, was conducted. Allergen-specific immunoglobulin E (IgE) was measured by using a multiplex allergen-specific IgE platform. Sensitization was defined\n as specific IgE level \u2265 0.35 kUA\/L. Associations among allergens were assessed by using the Fisher exact test with odds ratios (OR) and 95% confidence intervals. Quantitative relationships between nsLTP components were evaluated by using the Spearman correlation. Principal component analysis\n and k-means clustering were applied to identify sensitization profiles.\n Results:<\/jats:bold>\n Overall, 35.0% of the patients were sensitized to at least one nsLTP component. Strong associations were observed between Can s 3 and several food-derived nsLTPs, particularly Pru p 3 (peach)\n and Mal d 3 (apple) (ORs 222.9 and 227.6, respectively; p\u2009<\u20090.001). Can s 3 sensitization rarely occurred in isolation and was typically part of a broader nsLTP polysensitization pattern that involved both food and pollen allergens.\n Conclusion:<\/jats:bold>\n Can s 3\n sensitization seems integrated within the broader nsLTP sensitization landscape and is strongly associated with polysensitization to multiple nsLTP allergens. These findings support a shared nsLTP-mediated cross-reactivity network and highlight the relevance of including Can s 3 in molecular\n diagnostic panels for suspected nsLTP-mediated allergy.\n <\/jats:p>","DOI":"10.2500\/aap.2026.47.260045","type":"journal-article","created":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T04:50:20Z","timestamp":1788583820000},"page":"e118-e124","source":"Crossref","is-referenced-by-count":1,"title":["Cross-sensitization between\n Cannabis sativa<\/i>\n and Lipid transfer proteins: Evidence from a large molecular cohort"],"prefix":"10.2500","volume":"47","author":[{"given":"Carmen","family":"Frias Ruiz","sequence":"first","affiliation":[{"name":"From the Clinical Analysis Service, University Hospital Nuestra Se\u00f1ora de Candelaria, Santa Cruz de Tenerife, Spain and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria D.","family":"Martin Martinez","sequence":"additional","affiliation":[{"name":"From the Clinical Analysis Service, University Hospital Nuestra Se\u00f1ora de Candelaria, Santa Cruz de Tenerife, Spain and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gema","family":"Garcia de la Rosa","sequence":"additional","affiliation":[{"name":"From the Clinical Analysis Service, University Hospital Nuestra Se\u00f1ora de Candelaria, Santa Cruz de Tenerife, Spain and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1355","reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1111\/cea.14049","article-title":"Lipid transfer protein allergy: a review of current controversies","volume":"52","author":"Asero","year":"2022","journal-title":"Clin Exp Allergy"},{"key":"B2","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1111\/all.13043","article-title":"Cannabis sativa allergy: looking through the fog","volume":"72","author":"Decuyper","year":"2017","journal-title":"Allergy"},{"key":"B3","doi-asserted-by":"crossref","first-page":"e70064","DOI":"10.1111\/pai.70064","article-title":"IgE-mediated lipid transfer protein allergy in children","volume":"36","author":"Olivieri","year":"2025","journal-title":"Pediatr Allergy Immunol"},{"key":"B4","doi-asserted-by":"crossref","first-page":"2058738418803154","DOI":"10.1177\/2058738418803154","article-title":"Food-dependent, exercise-induced anaphylaxis in a patient allergic to peach","volume":"32","author":"Ukleja-Soko\u0142owska","year":"2018","journal-title":"Int J Immunopathol Pharmacol"},{"key":"B5","doi-asserted-by":"crossref","first-page":"2433","DOI":"10.1111\/all.14797","article-title":"The diagnosis and management of allergic reactions in patients sensitized to non-specific lipid transfer proteins","volume":"76","author":"Skypala","year":"2021","journal-title":"Allergy"},{"key":"B6","doi-asserted-by":"crossref","first-page":"1253304","DOI":"10.3389\/falgy.2023.1253304","article-title":"Factors and co-factors influencing clinical manifestations in nsLTPs allergy: between the good and the bad","volume":"4","author":"Ridolo","year":"2023","journal-title":"Front Allergy"},{"key":"B7","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.1111\/all.15237","article-title":"Cannabis-related allergies: an international overview and consensus recommendations","volume":"77","author":"Skypala","year":"2022","journal-title":"Allergy"},{"key":"B8","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1016\/j.jaip.2018.09.017","article-title":"Exploring the diagnosis and profile of Cannabis allergy","volume":"7","author":"Decuyper","year":"2019","journal-title":"J Allergy Clin Immunol Pract"},{"key":"B9","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.anai.2014.05.022","article-title":"IgE-binding properties of a recombinant lipid transfer protein from Cannabis sativa","volume":"113","author":"Rihs","year":"2014","journal-title":"Ann Allergy Asthma Immunol"},{"key":"B10","first-page":"2025","article-title":"International Union of Immunological Societies Allergen Nomenclature Sub-Committee. 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Allergen Nomenclature Database."},{"key":"B11","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1159\/000115887","article-title":"Sensitization and allergy to Cannabis sativa leaves in a population of tomato (Lycopersicon esculentum)-sensitized patients","volume":"146","author":"de Larramendi","year":"2008","journal-title":"Int Arch Allergy Immunol"},{"key":"B12","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1016\/j.jaci.2007.07.052","article-title":"Sensitization to Cannabis sativa caused by a novel allergenic lipid transfer protein, Can s 3","volume":"120","author":"Gamboa","year":"2007","journal-title":"J Allergy Clin Immunol"},{"key":"B13","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1093\/annweh\/wxaa043","article-title":"Cannabis: an emerging occupational allergen?","volume":"64","author":"Sussman","year":"2020","journal-title":"Ann Work Expo Health"},{"key":"B14","first-page":"AB12","article-title":"Cannabis hypersensitivity prevalence and presentation \u2013 A survey-based study of an allergic adult population in a Toronto Clinic at Onset of Canadian Legalization of Recreational Marijuana","volume":"145","author":"Lo","year":"2020","journal-title":"J Allergy Clin Immunol"},{"key":"B15","first-page":"954","article-title":"IgE-mediated allergy and asymptomatic sensitization to Cannabis allergens\u2014review of current knowledge and presentation of six cases","volume":"60","author":"W\u0105sik","year":"2024","journal-title":"Medicina (Lithuania)"},{"key":"B16","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.pneumo.2017.09.003","article-title":"Cannabis and crossed allergy with food","volume":"73","author":"Drouet","year":"2017","journal-title":"Rev de Pneumol Clin"},{"key":"B17","article-title":"ICH E6 Good Clinical Practice \u2013 Scientific guideline [Internet]","year":"2025"},{"key":"B18","article-title":"Cannabis Monograph 2022. 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