{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:15:17Z","timestamp":1790730917792,"version":"4.1.0"},"reference-count":12,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2006,5,1]],"date-time":"2006-05-01T00:00:00Z","timestamp":1146441600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["American Journal of Rhinology"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Eosinophils are a characteristic inflammatory cell infiltrate in both chronic rhinosinusitis (CRS) and sinonasal polyposis (SNP). The posttranslational modifications, 3-bromo-tyrosine (Br-Tyr) and 3-chloro-tyrosine (Cl-Tyr), serve as specific molecular markers for production of brominating and chlorinating oxidants, respectively, by the eosinophil peroxidase and myeloperoxidase systems of leukocytes. The aim of this study was to identify mechanisms of oxidative protein modifications in sinonasal mucosa of CRS and SNP patients by measuring Br-Tyr, Cl-Tyr, and alternative molecular markers of distinct oxidative pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Levels of Br-Tyr; Cl-Tyr; di-Tyrosine (di-Tyr), a specific oxidative cross-link; ortho-tyrosine (o-Tyr) and meta-tyrosine (m-Tyr), markers for protein modification by hydroxyl radical-like oxidants; and nitro-tyrosine (NO\n                      <jats:sub>2<\/jats:sub>\n                      -Tyr), a stable product of nitric oxide (NO)\u2013derived oxidants, were measured in anterior ethmoid mucosa tissue from CRS and SNP patients, as well as in middle turbinate mucosa from normal volunteers, using tandem mass spectrometry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Tissue levels of Br-Tyr were significantly higher in the CRS group compared with the control group (797 \u03bcmol\/mol versus 515 \u03bcmol\/mol tyrosine, p &lt; 0.015), but no differences were detected for Cl-Tyr, di-Tyr, m-Tyr, o-Tyr, and NO\n                      <jats:sub>2<\/jats:sub>\n                      -Tyr. Tissue levels of both Br-Tyr and di-Tyr were significantly higher in the SNP group compared with the control group (879 \u03bcmol\/mol versus 515 \u03bcmol\/mol, p &lt; 0.005; 5090 \u03bcmol\/mol versus 1700 \u03bcmol\/mol, p &lt; 0.024, respectively), but no differences were detected for Cl-Tyr, m-Tyr, o-Tyr, and NO\n                      <jats:sub>2<\/jats:sub>\n                      -Tyr.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Br-Tyr, a molecular footprint predominantly formed by eosinophil peroxidase\u2013catalyzed tissue damage, may serve as an objective index of CRS and SNP disease activity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2500\/ajr.2006.20.2858","type":"journal-article","created":{"date-parts":[[2006,8,21]],"date-time":"2006-08-21T05:54:51Z","timestamp":1156139691000},"page":"353-359","source":"Crossref","is-referenced-by-count":21,"title":["Characterization of Oxidative Pathways in Chronic Rhinosinusitis and Sinonasal Polyposis"],"prefix":"10.1177","volume":"20","author":[{"given":"Martin J.","family":"Citardi","sequence":"first","affiliation":[{"name":"Cleveland Clinic, Head and Neck Institute, Cleveland, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Song","sequence":"additional","affiliation":[{"name":"Cleveland Clinic, Department of Cell Biology, Cleveland, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pete S.","family":"Batra","sequence":"additional","affiliation":[{"name":"Cleveland Clinic, Head and Neck Institute, Cleveland, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald C.","family":"Lanza","sequence":"additional","affiliation":[{"name":"Sinus and Nasal Institute of Florida, St. Petersburg, Florida"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley L.","family":"Hazen","sequence":"additional","affiliation":[{"name":"Cleveland Clinic, Department of Cell Biology, Cleveland, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2006,5,1]]},"reference":[{"key":"bibr1-ajr.2006.20.2858","first-page":"S829","volume":"99","author":"Kaliner M.A.","year":"1997","journal-title":"Otolaryngol Head Neck Surg"},{"key":"bibr2-ajr.2006.20.2858","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.55.suppl_2.S79"},{"key":"bibr3-ajr.2006.20.2858","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.4049\/jimmunol.134.3.1875","volume":"134","author":"Carlson M.G.","year":"1985","journal-title":"J Immunol."},{"key":"bibr4-ajr.2006.20.2858","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.85.10.950"},{"key":"bibr5-ajr.2006.20.2858","doi-asserted-by":"publisher","DOI":"10.1179\/135100000101535771"},{"key":"bibr6-ajr.2006.20.2858","doi-asserted-by":"publisher","DOI":"10.1021\/bi982401l"},{"key":"bibr7-ajr.2006.20.2858","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.36.25933"},{"key":"bibr8-ajr.2006.20.2858","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112400200"},{"key":"bibr9-ajr.2006.20.2858","doi-asserted-by":"publisher","DOI":"10.1172\/JCI9702"},{"key":"bibr10-ajr.2006.20.2858","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.9.5763"},{"key":"bibr11-ajr.2006.20.2858","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8797"},{"key":"bibr12-ajr.2006.20.2858","doi-asserted-by":"publisher","DOI":"10.1053\/hn.2003.v128.amhn0312811"}],"container-title":["American Journal of Rhinology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.2500\/ajr.2006.20.2858","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.2500\/ajr.2006.20.2858","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T09:36:04Z","timestamp":1777368964000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.2500\/ajr.2006.20.2858"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5]]},"references-count":12,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.2500\/ajr.2006.20.2858"],"URL":"https:\/\/doi.org\/10.2500\/ajr.2006.20.2858","relation":{},"ISSN":["1050-6586","1539-6290"],"issn-type":[{"value":"1050-6586","type":"print"},{"value":"1539-6290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5]]}}}