{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:17:08Z","timestamp":1777385828468,"version":"3.51.4"},"reference-count":28,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Am J Rhinol\ufffdAllergy"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Remucoslization of the sinonasal cavity after sinus surgery is critical for successful outcomes. Recently, a novel antiprotease and antifibroblast compound, polyhydrated ionogen (PHI) with MgBr2, showed improved wound healing in a rabbit maxillary sinus mucosal wound model. We set out to determine if this effect was reproducible in an in vitro respiratory epithelial culture system.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Fully differentiated mature murine nasal septal air\u2013liquid interface cultures were injured by creating a full-thickness 400-mM-wide scratch through the monolayer. Cultures were then treated with nothing, saline, or PHI with MgBr\n                      <jats:sub>2<\/jats:sub>\n                      for 1 hour on the apical surface. Twenty-four hours after the injury cultures were fixed and processed for immunofluorescence with type IV beta-tubulin and Hoechst stain.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Initial injury resulted in a wound of 394 micromolar (377\u2013411 micromoler; 95% CI; n = 8). After 24 hours with no intervention the wound closed to 161 micromolar (138\u2013184 micromolar; 95% CI; n = 9) and treatment with saline resulted in a residual gap of 88 micromolar (60\u2013116 micromolar; 95% CI; n = 9; p &lt; 0.05) and treatment with PHI with MgBr\n                      <jats:sub>2<\/jats:sub>\n                      resulted in a gap of only 30 micromolar (14\u201346 micromolar; 95% CI; n = 9; p &lt; 0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Poor healing of the sinonasal mucosa after surgery with loss of ciliary function results in adverse clinical outcomes. In an in vitro sinonasal respiratory epithelial injury model, a one-time treatment with PHI with MgBr\n                      <jats:sub>2<\/jats:sub>\n                      showed significantly improved wound healing compared with saline or nothing. This is a viable model to further investigate the mechanism by which PHI with MgBr\n                      <jats:sub>2<\/jats:sub>\n                      improves sinonasal remucosolization.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.2500\/ajra.2013.25.3910","type":"journal-article","created":{"date-parts":[[2013,7,24]],"date-time":"2013-07-24T12:38:30Z","timestamp":1374669510000},"page":"333-337","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":4,"title":["Polyhydrated Ionogen with MgBr\n                    <sub>2<\/sub>\n                    Accelerates\n                    <i>in vitro<\/i>\n                    Respiratory Epithelial Healing"],"prefix":"10.1177","volume":"27","author":[{"given":"David","family":"DePoortere","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology\u2013Head and Neck Surgery, University of Pennsylvania, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2013Head and Neck Surgery, University of Pennsylvania, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noam A.","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2013Head and Neck Surgery, University of Pennsylvania, Philadelphia, Pennsylvania"},{"name":"Division of Otolaryngology\u2013Head and Neck Surgery, Philadelphia Veterans Administration Medical Center, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2018,4,27]]},"reference":[{"key":"bibr1-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1016\/j.iac.2009.07.004"},{"key":"bibr2-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.08.046"},{"key":"bibr3-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2006.01.016"},{"key":"bibr4-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1177\/00034894041130S503"},{"key":"bibr5-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2010.02.007"},{"key":"bibr6-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1985.00800110054002"},{"key":"bibr7-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.2500\/105065896781795067"},{"key":"bibr8-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.2001.00982.x"},{"key":"bibr9-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/5.3.242"},{"key":"bibr10-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1177\/000348949310200610"},{"key":"bibr11-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1177\/194589240201600202"},{"key":"bibr12-ajra.2013.25.3910","author":"Monroe S.","year":"2005","journal-title":"Effect of polyhydrated ionogen (PHI) on viability and matrix metalloproteinase levels in cultures of normal and diabetic human dermal fibroblasts."},{"key":"bibr13-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2003.12.10.26552"},{"key":"bibr14-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1080\/00015458.2007.11680145"},{"key":"bibr15-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2005.02079.x"},{"key":"bibr16-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1159\/000139397"},{"key":"bibr17-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1002\/alr.20019"},{"key":"bibr18-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1177\/0194599811399558"},{"key":"bibr19-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1016\/j.otohns.2010.06.712"},{"key":"bibr20-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.2144\/000112531"},{"key":"bibr21-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2007.21.3068"},{"key":"bibr22-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.2500\/ajr.2008.22.3125"},{"key":"bibr23-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.3233\/BIR-1992-295-606"},{"key":"bibr24-ajra.2013.25.3910","first-page":"L183","volume":"264","author":"Benali R.","year":"1993","journal-title":"Am J Physiol"},{"key":"bibr25-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199602)166:2<413::AID-JCP20>3.0.CO;2-A"},{"key":"bibr26-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1001\/archoto.2010.136"},{"key":"bibr27-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1097\/01.mlg.0000150674.30237.3f"},{"key":"bibr28-ajra.2013.25.3910","doi-asserted-by":"publisher","DOI":"10.1046\/j.1524-475X.1996.40404.x"}],"container-title":["American Journal of Rhinology &amp; 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