{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T14:20:12Z","timestamp":1777386012063,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Am J Rhinol\ufffdAllergy"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The naso-ocular interaction in allergic rhinoconjunctivitis is well recognized from epidemiological, clinical, and experimental observations. The precise mechanisms remain incompletely understood. A new mouse model of allergic rhinoconjunctivitis was used to investigate the contribution of mast cells and trigeminal ganglia activation to conjunctival (conj.) inflammation after nasal allergen provocation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Sensitized mice were exposed to ovalbumin (OVA) via the nose and\/or conjunctiva, and conj. homogenates were analyzed for histamine and substance P (using ELISA) and by eosinophil peroxidase (EPO) and beta-hexosaminidase assays. The conj. effects of nasal allergen deposition were compared with those induced by the mast cell activator C48\/80 and with pretreatment of the mast cell stabilizer ketotifen or the transient receptor potential channel receptor (TRP) agonist capsaicin. Protachykinin 1 (TAC1) expression was quantified in the trigeminal ganglia using real time polymerase chain reaction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>At 1 hour after nasal application of OVA, increased conj. levels of beta-hexosaminidase (0.68 \u00b1 0.03 nm versus 0.56 \u00b1 0.02 nm; p = 0.02), histamine (751.1 \u00b1 52.17 ng\/mL versus 546.3 \u00b1 76.91 ng\/mL; p = 0.05), and EPO (0.66 \u00b1 0.09 nm versus 0.37 \u00b1 0.03 nm; p = 0.02) were detected compared with saline. Higher levels of TAC1 expression were found in the trigeminal ganglia at 24 hours after OVA application (1326 \u00b1 255 versus 687.5 \u00b1 90.77 TAC1\/beta-actin; p = 0.04). Nasal challenge with C48\/80 increased substance P and beta-hexosaminidase levels in the conjunctiva, as well as TAC1 expression. Pretreatment with ketotifen resulted in lower levels of substance P as well as TAC1 expression. Destruction of sensory nerves in the nose by capsaicin reduced the OVA-induced conj. levels of substance P, histamine, a beta-hexosaminidase.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Nasal allergen deposition in sensitized mice induced trigeminal TAC1 expression and conj. mast cell degranulation. These data represent a significant step forward in understanding the close interaction between nasal and conj. inflammation in allergy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2500\/ajra.2014.28.4052","type":"journal-article","created":{"date-parts":[[2014,7,19]],"date-time":"2014-07-19T19:43:24Z","timestamp":1405799004000},"page":"290-296","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":11,"title":["Nasal Allergen Deposition Leads to Conjunctival Mast Cell Degranulation in Allergic Rhinoconjunctivitis"],"prefix":"10.1177","volume":"28","author":[{"given":"Ina","family":"Callebaut","sequence":"first","affiliation":[{"name":"Clinical Immunology, Department of Microbiology and Immunology, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annick","family":"De Vries","sequence":"additional","affiliation":[{"name":"Translational Research of Gastrointestinal Disorder, Catholic University of Leuven, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brecht","family":"Steelant","sequence":"additional","affiliation":[{"name":"Clinical Immunology, Department of Microbiology and Immunology, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valerie","family":"Hox","sequence":"additional","affiliation":[{"name":"Clinical Immunology, Department of Microbiology and Immunology, Leuven, Belgium"},{"name":"Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Leuven, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonja","family":"Bobic","sequence":"additional","affiliation":[{"name":"Clinical Immunology, Department of Microbiology and Immunology, Leuven, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Van Gerven","sequence":"additional","affiliation":[{"name":"Clinical Immunology, Department of Microbiology and 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