{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T08:30:28Z","timestamp":1787041828208,"version":"build-2736575974"},"reference-count":43,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Allergy\ufffdRhinol (Providence)"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Sensitization to Finales (Cupressaceae and Pinaceae) has increased dramatically in recent years. The prevalence of sensitization in different geographic areas is related to exposure to specific pollens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To investigate the prevalence of allergy to different conifer pollens, describe the characteristics of patients with such allergy, and identify the involved allergens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Patients were recruited at five hospitals near Madrid. Extracts from conifer pollen were prepared and used in skin-prick testing. Wheal sizes were recorded, and serum samples obtained from patients with positive reactions to Cupressus arizonica and\/or Pinus pinea. The specific immunoglobulin E value to C. arizonica and Cup a 1 was determined. Individual immunoblots for each patient and with a pool of sera were performed. Allergenic proteins were sequenced by using liquid chromatography-tandem mass spectrometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Of 499 individuals included in the study, 17 (14%) had positive skin-prick test results to some conifer pollen extracts. Sixty-four patients had positive results to C. arizonica (prevalence 12.8%) and 11 had positive results to P. pinea (2.2%). All the patients had respiratory symptoms (61.4% during the C. arizonica pollination period), and 62.9% had asthma. Approximately 86% of the patients had positive specific immunoglobulin E results to C. arizonica and 923% had positive results to Cup a 1. Fourteen different bands were recognized by immunoblot; the most frequent bands were those detected at 43, 18, 16, and 14 kDa. All sequenced proteins corresponded to Cup a 1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Allergy to conifer pollen could be considered a relevant cause of respiratory allergy in central Spain. Asthma was more frequent than in other studies. We only identified Cup a 1 as involved in sensitization.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2500\/ar.2016.7.0183","type":"journal-article","created":{"date-parts":[[2017,1,11]],"date-time":"2017-01-11T08:23:22Z","timestamp":1484123002000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Prevalence of Allergic Sensitization to Conifer Pollen in a High Cypress Exposure Area"],"prefix":"10.1177","volume":"7","author":[{"given":"Javier","family":"Dom\u00ednguez-Ortega","sequence":"first","affiliation":[{"name":"Allergy Unit, Hospital Universitario La Paz, Institute for Health Research (IdiPAZ), Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda \u00c1ngeles","family":"L\u00f3pez-Matas","sequence":"additional","affiliation":[{"name":"R&D Department, Laboratorios LETI S.L.U., Tres Cantos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Dolores","family":"Alonso","sequence":"additional","affiliation":[{"name":"Allergy Unit, Hospital Universitario Fundaci\u00f3n Alcorc\u00f3n, Alcorc\u00f3n, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e9lica","family":"Feliu","sequence":"additional","affiliation":[{"name":"Allergy Unit, Hospital del Tajo, Aranjuez, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Ruiz-Hornillos","sequence":"additional","affiliation":[{"name":"Allergy Unit, Hospital Universitario Infanta Elena, Valdemoro, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Allergy Unit, Hospital Infanta Cristina, Parla, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Moya","sequence":"additional","affiliation":[{"name":"R&D Department, Laboratorios LETI S.L.U., Tres Cantos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jer\u00f3nimo","family":"Carn\u00e9s","sequence":"additional","affiliation":[{"name":"R&D Department, Laboratorios LETI S.L.U., Tres Cantos, Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,10,1]]},"reference":[{"key":"bibr1-ar.2016.7.0183","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69283-0"},{"key":"bibr2-ar.2016.7.0183","doi-asserted-by":"publisher","DOI":"10.1111\/cea.12439"},{"key":"bibr3-ar.2016.7.0183","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02646.x"},{"key":"bibr4-ar.2016.7.0183","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2005.00731.x"},{"key":"bibr5-ar.2016.7.0183","first-page":"14","volume":"19","author":"Quirce S.","year":"2009","journal-title":"J Investig Allergol Clin Immunol"},{"key":"bibr6-ar.2016.7.0183","doi-asserted-by":"publisher","DOI":"10.1159\/000053828"},{"key":"bibr7-ar.2016.7.0183","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2012.06.018"},{"key":"bibr8-ar.2016.7.0183","first-page":"205","volume":"15","author":"Rocha-Estrada A.","year":"2008","journal-title":"Ann Agric Environ Med"},{"key":"bibr9-ar.2016.7.0183","first-page":"352","volume":"26","author":"Deane P.M.","year":"2005","journal-title":"Allergy Asthma Proc"},{"key":"bibr10-ar.2016.7.0183","doi-asserted-by":"publisher","DOI":"10.2500\/aap.2010.31.3352"},{"key":"bibr11-ar.2016.7.0183","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.11.002"},{"key":"bibr12-ar.2016.7.0183","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2000.00949.x"},{"key":"bibr13-ar.2016.7.0183","unstructured":"WHO\/IUIS Allergen Nomenclature Sub-committee. http:\/\/www.allergen.org\/."},{"key":"bibr14-ar.2016.7.0183","unstructured":"Allergome. 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