{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T12:54:48Z","timestamp":1774788888845,"version":"3.50.1"},"reference-count":0,"publisher":"University of Toronto Press Inc. (UTPress)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical and Investigative Medicine"],"published-print":{"date-parts":[[2013,4,1]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p> The purpose of this study was to evaluate Interferon (IFN)-\u03b3 and Interleukin(IL)-17 profiles in patients with different clinical presentations of pulmonary tuberculosis (TB) and to compare them with those of tuberculin-negative and tuberculin-reactive healthy controls <\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p> Peripheral blood mononuclear cells (PBMCss), isolated from patients (n=52) and controls (n=30), were stimulated ex vivo with purified protein derivative (PPD) and IFN-\u03b3 and IL-17 levels in the supernatant were measured. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> At baseline, PBMCss from patients with TB released a significantly lower amount of IL-17 (p=0.043) than PBMCss from healthy controls, whereas IFN-\u03b3 levels were similar in the two groups. After PPD stimulation, a significant rise in IL-17 levels was found only among healthy controls (p=0.02). This rise in IL-17 levels was similar between tuberculin-reactive and tuberculin-negative subjects. After PPD stimulation, patients with infiltrative TB secreted higher levels of IL-17 and IFN-\u03b3 than those affected with chronic, miliary and cavitary TB (p &lt; 0.01). IFN-\u03b3 production from patients with infiltrative TB was even higher than for healthy controls (p &lt; 0.01). PBMCss from tuberculin-reactive patients released higher levels of IFN-\u03b3 than tuberculin-negative subjects after PPD stimulation (p &lt; 0.01). <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p> Ex vivo PPD stimulation of PBMCs from patients with pulmonary TB does not significantly stimulate IL-17 release; however, higher IL-17 and IFN-\u03b3 levels are found in patients with infiltrative disease, in comparison with those affected with miliary, cavitary and chronic TB. <\/jats:p><\/jats:sec>","DOI":"10.25011\/cim.v36i2.19568","type":"journal-article","created":{"date-parts":[[2017,5,2]],"date-time":"2017-05-02T23:09:59Z","timestamp":1493766599000},"page":"E64-E71","source":"Crossref","is-referenced-by-count":11,"title":["Interferon-\u03b3 and Interleukin-17 production from PPD-stimulated PBMCss of patients with pulmonary tuberculosis"],"prefix":"10.3138","volume":"36","author":[{"given":"Giuseppe","family":"Nunnari","sequence":"first","affiliation":[{"name":"Department of Clinical and Molecular Biomedicine, Division of Infectious Diseases, University of Catania, Catania 95125, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marilia R","family":"Pinzone","sequence":"additional","affiliation":[{"name":"Department of Clinical and Molecular Biomedicine, Division of Infectious Diseases, University of Catania, Catania 95125, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlo","family":"Vancheri","sequence":"additional","affiliation":[{"name":"Department of Clinical and Molecular Biomedicine, Unit of Chest Diseases, University of Catania, Catania 95125, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filippo","family":"Palermo","sequence":"additional","affiliation":[{"name":"Department of Clinical and Molecular Biomedicine, Division of Infectious Diseases, University of Catania, Catania 95125, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Cacopardo","sequence":"additional","affiliation":[{"name":"Department of Clinical and Molecular Biomedicine, Division of Infectious Diseases, University of Catania, Catania 95125, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1696","container-title":["Clinical and Investigative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/cimonline.ca\/index.php\/cim\/article\/viewFile\/19568\/16201","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/utppublishing.com\/doi\/pdf\/10.25011\/cim.v36i2.19568","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T18:34:42Z","timestamp":1741890882000},"score":1,"resource":{"primary":{"URL":"https:\/\/utppublishing.com\/doi\/10.25011\/cim.v36i2.19568"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,4,1]]}},"alternative-id":["10.25011\/cim.v36i2.19568"],"URL":"https:\/\/doi.org\/10.25011\/cim.v36i2.19568","relation":{},"ISSN":["1488-2353"],"issn-type":[{"value":"1488-2353","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,1]]}}}