{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T04:27:21Z","timestamp":1741926441651,"version":"3.38.0"},"reference-count":0,"publisher":"University of Toronto Press Inc. (UTPress)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical and Investigative Medicine"],"published-print":{"date-parts":[[2014,4,1]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p> GRIM-19 has been shown to be down-regulated in cervical cancers. This study investigated the expression of GRIM-19 in cervical intra-epithelial neoplasias and its association with high-risk human papillomavirus (HR-HPV) infection. <\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p> The expression of GRIM-19 was assessed in cervical exfoliated cells and cervical intra-epithelial neoplasia tissues by immunohistochemistry, and the level of GRIM-19 was also evaluated by Western blotting using cervical exfoliated cells. HR-HPV infection of cervical exfoliated cells was detected by HC II. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> GRIM-19 is predominantly expressed in the cytoplasm of the middle layer of normal cervical epithelial cells, whereas the surface layer cells of the normal cervix showed no GRIM-19 expression. The expression of GRIM-19 gradually decreased from atypical squamous cells of undetermined significance (ASCUS), low-grade squamous intraepithelial lesion (LSIL) and high-grade squamous intraepithelial lesion (HSIL) to squamous cell carcinoma (SCC); a pattern which was also observed in cervical intra-epithelial neoplasias tissues. The reduced expression of GRIM-19 was correlated with HR-HPV infection. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p> GRIM-19 may regulate the differentiation of normal cervical tissue, and a decrease in GRIM-19 may be the result of HR-HPV infection, which in turn leads to the malignant transformation of the cells. <\/jats:p><\/jats:sec>","DOI":"10.25011\/cim.v37i2.21089","type":"journal-article","created":{"date-parts":[[2017,5,2]],"date-time":"2017-05-02T12:50:36Z","timestamp":1493729436000},"page":"E77-E84","source":"Crossref","is-referenced-by-count":3,"title":["Decreased expression of GRIM-19 and its association with high-risk HPV infection in cervical squamous intraepithelial neoplasias and cancer"],"prefix":"10.3138","volume":"37","author":[{"given":"Yong","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology, Shandong, Cancer Hospital and Institute, Shandong University, Jinan, Shandong Province 250117, P.R. China"},{"name":"Department of Oncological Radiotherapy, Anhui Provincial Hospital Affiliated with Anhui, Medical University, Hefei, 230001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong-yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oncological Radiotherapy, Anhui Provincial Hospital Affiliated with Anhui, Medical University, Hefei, 230001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Anhui Provincial Hospital Affiliated with Anhui, Medical University, Hefei, 230001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feng","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Anhui Provincial Hospital Affiliated with Anhui, Medical University, Hefei, 230001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong-hua","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, Shandong, Cancer Hospital and Institute, Shandong University, Jinan, Shandong Province 250117, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1696","container-title":["Clinical and Investigative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/cimonline.ca\/index.php\/cim\/article\/viewFile\/21089\/17193","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/utppublishing.com\/doi\/pdf\/10.25011\/cim.v37i2.21089","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T18:23:40Z","timestamp":1741890220000},"score":1,"resource":{"primary":{"URL":"https:\/\/utppublishing.com\/doi\/10.25011\/cim.v37i2.21089"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,4,1]]}},"alternative-id":["10.25011\/cim.v37i2.21089"],"URL":"https:\/\/doi.org\/10.25011\/cim.v37i2.21089","relation":{},"ISSN":["1488-2353"],"issn-type":[{"type":"electronic","value":"1488-2353"}],"subject":[],"published":{"date-parts":[[2014,4,1]]}}}