{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:45:36Z","timestamp":1789659936542,"version":"4.0.1"},"reference-count":0,"publisher":"University of Toronto Press Inc. (UTPress)","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical and Investigative Medicine"],"published-print":{"date-parts":[[2014,10,4]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p> Aberrant expression of proteins involved in epithelial-to-mesenchymal transition have been described in various cancers. In this retrospective study, we sought to evaluate E-cadherin, \u03b2-catenin and vimentin protein expression in non-metastatic nasopharyngeal (NPC) patients treated with curative intent, examine their relationship with each other, and with clinical outcome measures. <\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p> Pre-treatment formalin-fixed paraffin-embedded biopsies of 140 patients treated between January 2000 and December 2007 were assembled into a tissue microarray (TMA). Automated quantitative immunohistochemistry (AQUA\u00ae) was performed on sequential TMA sections stained with fluorescent-labeled antibodies against E-cadherin, \u03b2-catenin and vimentin. Cox proportional hazards regression was used to estimate the effect of cytoplasmic vimentin, cytoplasmic E-cadherin, \u03b2-catenin nuclear\/cytoplasmic ratio expression on overall survival and disease-free survival. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> The average age of the patients was 51.7 years (SD=12.1; range 18-85), 66% were male, 71% had a KPS \u2265 90% at the start of treatment and 65% had stage III\/IV disease. After adjusting for performance status, WHO and stage, high E-cadherin levels over the 75th percentile were found to produce a significantly increased risk for both a worse overall survival (HR = 2.53, 95% CI 1.21, 5.27) and disease free survival (DFS; HR = 2.14, 95%CI 1.28, 3.59). Vimentin levels over the first quartile produced an increased risk for a worse DFS (HR = 2.21, 95% CI 1.11, 4.38). No association was seen between \u03b2-catenin and survival. <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p> In this cohort of NPC patients, higher levels of E-cadherin and higher levels of vimentin were associated with worse outcomes. Further work is needed to understand the role of these epithelial mesenchymal transition proteins in NPC. <\/jats:p><\/jats:sec>","DOI":"10.25011\/cim.v37i5.22012","type":"journal-article","created":{"date-parts":[[2017,5,2]],"date-time":"2017-05-02T12:47:09Z","timestamp":1493729229000},"page":"E320-E330","source":"Crossref","is-referenced-by-count":12,"title":["Evaluation of E-cadherin, \u03b2-catenin and vimentin protein expression using quantitative immunohistochemistry in nasopharyngeal carcinoma patients"],"prefix":"10.3138","volume":"37","author":[{"given":"Desir\u00e9e","family":"Hao","sequence":"first","affiliation":[{"name":"Department of Medical Oncology, Tom Baker Cancer Centre, Calgary, Alberta, Canada"},{"name":"Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tien","family":"Phan","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada"},{"name":"Department of Radiation Oncology, Tom Baker Cancer Centre, Calgary, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"Jagdis","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jodi E.","family":"Siever","sequence":"additional","affiliation":[{"name":"Senior Analyst\/Biostatistics, Research & Innovation, Population, Public, and Aboriginal Health, Alberta Health Services"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander C.","family":"Klimowicz","sequence":"additional","affiliation":[{"name":"Department of Pathology, Tom Baker Cancer Centre, Calgary, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janessa J.","family":"Laskin","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada"},{"name":"Department of Medical Oncology, British Columbia Cancer Agency \u2013 Vancouver"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Thomson","sequence":"additional","affiliation":[{"name":"Pathologist, British Columbia Cancer Agency \u2013 Vancouver and Faculty of Medicine, University of British Columbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Sarah","family":"Rose","sequence":"additional","affiliation":[{"name":"Rho - Sigma Scientific Consultants Calgary, Calgary, AB, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie K.","family":"Petrillo","sequence":"additional","affiliation":[{"name":"Department of Pathology, Tom Baker Cancer Centre, Calgary, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony M.","family":"Magliocco","sequence":"additional","affiliation":[{"name":"Department of Anatomic Pathology, H. Lee Moffitt Cancer Center, Tampa, Florida, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harold Y.","family":"Lau","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada"},{"name":"Department of Radiation Oncology, Tom Baker Cancer Centre, Calgary, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1696","container-title":["Clinical and Investigative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/cimonline.ca\/index.php\/cim\/article\/viewFile\/22012\/17853","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/utppublishing.com\/doi\/pdf\/10.25011\/cim.v37i5.22012","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T18:27:05Z","timestamp":1741890425000},"score":1,"resource":{"primary":{"URL":"https:\/\/utppublishing.com\/doi\/10.25011\/cim.v37i5.22012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,10,4]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,10,4]]}},"alternative-id":["10.25011\/cim.v37i5.22012"],"URL":"https:\/\/doi.org\/10.25011\/cim.v37i5.22012","relation":{},"ISSN":["1488-2353"],"issn-type":[{"value":"1488-2353","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,10,4]]}}}