{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T18:02:13Z","timestamp":1773252133387,"version":"3.50.1"},"reference-count":0,"publisher":"University of Toronto Press Inc. (UTPress)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical and Investigative Medicine"],"published-print":{"date-parts":[[2023,9,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p> H79 is the first long noncoding RNA (IncRNA) found to be associated with gene imprinting. It is highly expressed in the embryonic stage and may have important regulatory effects on human embryonic development. We investigated the differences between the levels of H79 promoter DNA methylation in the chorionic villi of patients who experienced spontaneous abortion (SA) following in vitro fertilization embryo transfer (IVF-ET) and those of patients with a normal early pregnancy (NEP). We also analyzed the associated DNA methyltransferase (DNMT) activity. <\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p> Chorionic villus tissue from patients with SA and NEP were collected. The DNA methylation levels of two CpG islands in the promoter region of the H19 gene in the two groups were detected by bisulfite sequencing, and the mRNA expression of DNMTs was analyzed by real-time polymerase chain reaction. <\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> The sample size of each group was 32, and there were no significant differences in baseline data, including age, parity, and body mass index, between the two groups. Among the 7 CpG islands measured, the methylation rates of 3 CpG islands (CpG 1, 6, and 7) were significantly lower in the SA group than in the NEP group (P &lt; 0.01). The methylation levels of the other 4 CpG islands were not significantly different between the two groups. There were no differences in the expression of DNMT1 between the two groups (P &gt; 0.05), but DNMT3a and DNMT3b RNA levels were significantly lower in SA group than in the NEP group (P &lt; 0.01). <\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p> The lower H19 promoter DNA methylation levels found in the chorionic villi of patients with SA patients following IVF-ET may be explained by decreased expression of DNMT3a and DNMT3b. <\/jats:p><\/jats:sec>","DOI":"10.25011\/cim.v46i3.41654","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T20:25:06Z","timestamp":1695932706000},"page":"E13-E18","source":"Crossref","is-referenced-by-count":2,"title":["<i>H19<\/i> Promoter DNA Methylation is Lower Among Early Abortion Patients Undergoing IVF Embryo Transfer"],"prefix":"10.3138","volume":"46","author":[{"given":"Lei","family":"Han","sequence":"first","affiliation":[{"name":"Department of Reproductive Medicine, Binzhou Medical university Hospital, Binzhou city, Shandong Province, 256603, China"},{"name":"Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Chongqing Medical University, Chongqing, 401120, China"},{"name":"Department of Reproductive Medicine, Maternal and Child Health Hospital Affiliated to Zunyl Medical University, Zunyl City, Guizhou Province, 563000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianghui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Binzhou Medical university Hospital, Binzhou city, Shandong Province, 256603, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Binzhou Medical university Hospital, Binzhou city, Shandong Province, 256603, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huishu","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Binzhou Medical university Hospital, Binzhou city, Shandong Province, 256603, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baolin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Binzhou Medical university Hospital, Binzhou city, Shandong Province, 256603, China"},{"name":"Department Of Obstetrics and Gynecology, Binzhou Central Hospital, Binzhou City, Shandong Province, 251700, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiwei","family":"Pang","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Binzhou Medical university Hospital, Binzhou city, Shandong Province, 256603, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peihui","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Binzhou Medical university Hospital, Binzhou city, Shandong Province, 256603, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caiying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Postgraduate Student Office, Binzhou Medical University Hospital, Binzhou City, Shandong Province, 256603, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanlin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine, Binzhou Medical university Hospital, Binzhou city, Shandong Province, 256603, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiajia","family":"An","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Binzhou Medical University Hospital, Binzhou City, Shandong Province, 256603, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1696","container-title":["Clinical and Investigative Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cimonline.ca\/index.php\/cim\/article\/download\/41654\/31986","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/utppublishing.com\/doi\/pdf\/10.25011\/cim.v46i3.41654","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T18:09:41Z","timestamp":1741889381000},"score":1,"resource":{"primary":{"URL":"https:\/\/utppublishing.com\/doi\/10.25011\/cim.v46i3.41654"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,9,1]]}},"alternative-id":["10.25011\/cim.v46i3.41654"],"URL":"https:\/\/doi.org\/10.25011\/cim.v46i3.41654","relation":{},"ISSN":["1488-2353"],"issn-type":[{"value":"1488-2353","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,1]]}}}