{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T09:20:20Z","timestamp":1760347220862},"reference-count":0,"publisher":"Universidad del Valle","issue":"3","license":[{"start":{"date-parts":[[2018,9,28]],"date-time":"2018-09-28T00:00:00Z","timestamp":1538092800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"},{"start":{"date-parts":[[2018,9,28]],"date-time":"2018-09-28T00:00:00Z","timestamp":1538092800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/deed.es"}],"content-domain":{"domain":["colombiamedica.univalle.edu.co"],"crossmark-restriction":true},"short-container-title":["Colomb Med"],"abstract":"<jats:p>Background: The yeasts species determination is fundamental not only for an accurate diagnosis but also for establishing a suitable patient treatment. We performed a concordance study of five methodologies for the species identification of oral isolates of Candida in Colombia.\r\nMethods: Sixty-seven Candida isolates were tested by; API\u00ae 20C-AUX, Vitek\u00ae2 Compact, Vitek\u00aeMS, Microflex\u00ae and a molecular test (panfungal PCR and sequencing). The commercial cost and processing time of the samples was done by graphical analysis.\r\nResults: Panfungal PCR differentiated 12 species of Candida, Vitek\u00aeMS and Microflex\u00ae methods identified 9 species, and API\u00ae 20C-AUX and Vitek\u00ae2 Compact methods identified 8 species each. Weighted Kappa (wK) showed a high agreement between Panfungal PCR, Vitek\u00aeMS, Microflex\u00ae and API\u00ae 20C-AUX (wK 0.62-0.93). The wK that involved the Vitek\u00ae2 Compact method presented moderate or good concordances compared with the other methods (wK 0.56-0.73). Methodologies based on MALDI TOF MS required 4 minutes to generate results and the Microflex\u00ae method had the lowest selling price.\r\nConclusion: The methods evaluated showed high concordance in their results, being higher for the molecular methods and the methodologies based on MALDI TOF. The latter are faster and cheaper, presenting as promising alternatives for the routine identification of yeast species of the genus Candida.<\/jats:p>","DOI":"10.25100\/cm.v49i3.3774","type":"journal-article","created":{"date-parts":[[2018,11,29]],"date-time":"2018-11-29T22:43:21Z","timestamp":1543531401000},"page":"193-200","update-policy":"http:\/\/dx.doi.org\/10.25100\/cm.v43i.2","source":"Crossref","is-referenced-by-count":0,"title":["Concordance analysis between different methodologies used for identification of oral isolates of Candida species"],"prefix":"10.25100","volume":"49","author":[{"given":"Natalia","family":"Maldonado","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luz","family":"Cano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandra","family":"Zuluaga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Arango Bustamante","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Caceres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zilpa","family":"S\u00e1nchez Quitian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica","family":"Vel\u00e1squez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"G\u00f3mez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Parra Giraldo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catalina","family":"De Bedout","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ra\u00fal","family":"Rivera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10728","published-online":{"date-parts":[[2018,9,28]]},"container-title":["Colombia Medica"],"original-title":[],"link":[{"URL":"https:\/\/colombiamedica.univalle.edu.co\/index.php\/comedica\/article\/download\/3909\/6635","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/colombiamedica.univalle.edu.co\/index.php\/comedica\/article\/download\/3909\/4376","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/colombiamedica.univalle.edu.co\/index.php\/comedica\/article\/download\/3909\/6637","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/colombiamedica.univalle.edu.co\/index.php\/comedica\/article\/download\/3909\/6636","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/colombiamedica.univalle.edu.co\/index.php\/comedica\/article\/download\/3909\/6634","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,14]],"date-time":"2024-08-14T22:23:47Z","timestamp":1723674227000},"score":1,"resource":{"primary":{"URL":"https:\/\/colombiamedica.univalle.edu.co\/index.php\/comedica\/article\/view\/3909"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,28]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2018,9,28]]}},"URL":"https:\/\/doi.org\/10.25100\/cm.v49i3.3774","relation":{},"ISSN":["1657-9534","0120-8322"],"issn-type":[{"type":"electronic","value":"1657-9534"},{"type":"print","value":"0120-8322"}],"subject":[],"published":{"date-parts":[[2018,9,28]]},"assertion":[{"value":"Yes","name":"crosschecked","label":"CrossChecked"},{"value":"Yes","name":"peer_reviewed","label":"Peer Reviewed"}]}}