{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:54:22Z","timestamp":1787025262786,"version":"build-2736575974"},"reference-count":11,"publisher":"Universidad del Valle","issue":"2","license":[{"start":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T00:00:00Z","timestamp":1693440000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0"}],"content-domain":{"domain":["colombiamedica.univalle.edu.co"],"crossmark-restriction":true},"short-container-title":["Colomb Med"],"abstract":"<jats:p>Introducci\u00f3n:Anemias megalobl\u00e1sticas secundarias a la deficiencia de vitamina B12 son patolog\u00edas producidas por una s\u00edntesis defectuosa del ADN nuclear.\nObjetivo:Describir las alteraciones madurativas encontradas en precursores hematopoy\u00e9ticos de la m\u00e9dula \u00f3sea de una serie de pacientes con anemia megalobl\u00e1stica.\nM\u00e9todos:Se incluyeron pacientes atendidos en el Hospital Regional de Concepci\u00f3n con muestras de m\u00e9dula \u00f3sea enviadas para estudio de citopenias por citometr\u00eda de flujo cuyo diagn\u00f3stico fue anemia megalobl\u00e1stica. El inmunofenotipo se realiz\u00f3 con CD45, CD34, CD117, HLA-DR, marcadores de maduraci\u00f3n de serie de neutr\u00f3filo (CD13, CD11b, CD10, CD16) y\/o eritroblasto (CD105, CD71, CD36).\nResultados:Se identificaron 8 pacientes con anemia megalobl\u00e1stica y como controles se utilizaron s\u00edndromes mielodispl\u00e1sicos (n=9) y m\u00e9dula \u00f3sea normal o reactiva (n=10). El 44% eran hombres, con una mediana de edad de 58 a\u00f1os. La anemia megalobl\u00e1stica se asoci\u00f3 con una mayor proporci\u00f3n de tama\u00f1o y complejidad de progenitores eritroides y mieloides con respecto de los linfocitos en comparaci\u00f3n a los controles. El porcentaje total de eritroblastos y la proporci\u00f3n de c\u00e9lulas mieloides CD34+ comprometidas con el linaje eritroide fue mayor en anemia megalobl\u00e1stica, asociado a una parada madurativa en la etapa de precursor CD105+ (69% vs 19% y 23%, p &lt;0.001). La heterogeneidad de CD36 y CD71 en anemia megalobl\u00e1stica fue similar a los s\u00edndromes mielodispl\u00e1sicos\nConclusiones:La anemia megalobl\u00e1stica produce una afectaci\u00f3n heterog\u00e9nea de la hematopoyesis, caracterizada por un mayor tama\u00f1o y complejidad celulares de precursores de la serie neutr\u00f3filo y eritroide y una detenci\u00f3n madurativa de los eritroblastos<\/jats:p>","DOI":"10.25100\/cm.v54i2.5494","type":"journal-article","created":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T12:07:35Z","timestamp":1694779655000},"page":"e2005494","update-policy":"https:\/\/doi.org\/10.25100\/cm.crossmarkpolicy","source":"Crossref","is-referenced-by-count":5,"title":["Cambios detectados por citometr\u00eda de flujo en la anemia megalobl\u00e1stica secundaria a la deficiencia de cobalamina"],"prefix":"10.25100","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-1731-5828","authenticated-orcid":false,"given":"Vania Bel\u00e9n","family":"Briones Mu\u00f1oz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6023-6113","authenticated-orcid":false,"given":"Fernanda Isabel","family":"Figueroa Fa\u00fandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4700-7360","authenticated-orcid":false,"given":"Catalina Monserrat","family":"Vidal Rojas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7047-4994","authenticated-orcid":false,"given":"Vicente Tom\u00e1s","family":"Micolich Espejo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7047-4994","authenticated-orcid":false,"given":"Mauricio Andr\u00e9s","family":"Chandia Cabas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10728","published-online":{"date-parts":[[2023,8,31]]},"reference":[{"key":"59382","doi-asserted-by":"crossref","unstructured":"Koury MJ, Horne DW, Brown ZA, et al. Apoptosis of late-stage erythroblasts in megaloblastic anemia:association with DNA damage and macrocyte production. Blood. 1997; 89(12): 4617-4623. https:\/\/doi.org\/10.1182\/blood.V89.12.4617 PMid:9192787","DOI":"10.1182\/blood.V89.12.4617"},{"key":"59383","unstructured":"Ballas SK, Pielichowski HJ, Stoll DB. Further characterization of the erythrocyte membrane protein abnormality in megaloblastic anemia. J Med. 1982;13(1-2):15-34."},{"key":"59384","doi-asserted-by":"crossref","unstructured":"Stabler SP. Vitamin B12 Deficiency. N Engl J Med. 2013; 368(2): 149-160. https:\/\/doi.org\/10.1056\/NEJMcp1113996 PMid:23301732","DOI":"10.1056\/NEJMcp1113996"},{"key":"59385","doi-asserted-by":"crossref","unstructured":"Green R. Vitamin B12 deficiency from the perspective of a practicing hematologist. Blood. 2017; 129(19): 2603-2611. https:\/\/doi.org\/10.1182\/blood-2016-10-569186 PMid:28360040","DOI":"10.1182\/blood-2016-10-569186"},{"key":"59386","doi-asserted-by":"crossref","unstructured":"Sutton L, Mba N. Hematogones detected by flow cytometry in a child with vitamin B12 deficiency: a case report. Pediatr Dev Pathol. 2017; 20(2):172-175. https:\/\/doi.org\/10.1177\/1093526616686241 PMid:28326965","DOI":"10.1177\/1093526616686241"},{"key":"59387","doi-asserted-by":"crossref","unstructured":"van Dongen JJM, Lhermitte L, B\u00f6ttcher S, Almeida J, van der Velden VHJ, Flores-Montero J, et al. EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia. 2012; 26(9): 1908-1975. https:\/\/doi.org\/10.1038\/leu.2012.120 PMid:22552007 PMCid:PMC3437410","DOI":"10.1038\/leu.2012.120"},{"key":"59388","doi-asserted-by":"crossref","unstructured":"Matarraz S, L\u00f3pez A, Barrena S, Fernandez C, Jensen E, Flores-Montero J,et al. Bone marrow cells from myelodysplastic syndromes show altered immunophenotypic profiles that may contribute to the diagnosis and prognostic stratification of the disease: a pilot study on a series of 56 patients. Cytometry B Clin Cytom. 2010; 78(3): 154-168. https:\/\/doi.org\/10.1002\/cyto.b.20513 PMid:20198685","DOI":"10.1002\/cyto.b.20513"},{"key":"59389","doi-asserted-by":"crossref","unstructured":"Orfao A, Matarraz S, P\u00e9rez-Andr\u00e9s M, Almeida J, Teodosio C, Berkowska MA, et al. Immunophenotypic dissection of normal hematopoiesis. J Immunol Methods. 2019;475:112684. https:\/\/doi.org\/10.1016\/j.jim.2019.112684 PMid:31676343","DOI":"10.1016\/j.jim.2019.112684"},{"key":"59390","doi-asserted-by":"crossref","unstructured":"Mello FV, Land MGP, Costa ES, Teod\u00f3sio C, Sanchez ML, B\u00e1rcena P, et al. Maturation-associated gene expression profiles during normal human bone marrow erythropoiesis. Cell Death Discov. 2019;5(1):1-12. https:\/\/doi.org\/10.1038\/s41420-019-0151-0 PMid:30854228 PMCid:PMC6395734","DOI":"10.1038\/s41420-019-0151-0"},{"key":"59391","doi-asserted-by":"crossref","unstructured":"Green R, Datta Mitra A. Megaloblastic anemias: nutritional and other causes. Med Clin North Am. 2017;101(2):297-317. https:\/\/doi.org\/10.1016\/j.mcna.2016.09.013 PMid:28189172","DOI":"10.1016\/j.mcna.2016.09.013"},{"key":"59392","doi-asserted-by":"crossref","unstructured":"Matarraz S, L\u00f3pez A, Barrena S, Fernandez C, Jensen E, Flores J, et al. The immunophenotype of different immature, myeloid and B-cell lineage-committed CD34+ hematopoietic cells allows discrimination between normal\/reactive and myelodysplastic syndrome precursors. Leukemia. 2008; 22(6): 1175-1183. https:\/\/doi.org\/10.1038\/leu.2008.49 PMid:18337765","DOI":"10.1038\/leu.2008.49"}],"container-title":["Colombia Medica"],"original-title":[],"link":[{"URL":"https:\/\/colombiamedica.univalle.edu.co\/index.php\/comedica\/article\/download\/5494\/5497","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/colombiamedica.univalle.edu.co\/index.php\/comedica\/article\/download\/5494\/5498","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/colombiamedica.univalle.edu.co\/index.php\/comedica\/article\/download\/5494\/5500","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/colombiamedica.univalle.edu.co\/index.php\/comedica\/article\/download\/5494\/5499","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/colombiamedica.univalle.edu.co\/index.php\/comedica\/article\/download\/5494\/5498","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T13:59:03Z","timestamp":1722952743000},"score":1,"resource":{"primary":{"URL":"https:\/\/colombiamedica.univalle.edu.co\/index.php\/comedica\/article\/view\/5494"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,31]]},"references-count":11,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,7,24]]}},"URL":"https:\/\/doi.org\/10.25100\/cm.v54i2.5494","relation":{},"ISSN":["1657-9534"],"issn-type":[{"value":"1657-9534","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,31]]},"assertion":[{"value":"Yes","name":"crosschecked","label":"CrossChecked"},{"value":"Yes","name":"peer_reviewed","label":"Peer Reviewed"}]}}