{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:17:59Z","timestamp":1790381879092,"version":"4.1.0"},"reference-count":21,"publisher":"S.C. JURNALUL PENTRU MEDICINA SI VIATA S.R.L","issue":"2","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMedLife"],"abstract":"<jats:p><jats:italic>Aspergillus<\/jats:italic> species (sp.) that causes opportunistic infections have been increasingly found in human mainly immunosuppressive patients around the world every year. The main objective was to use a rapid and cheap molecular method for monitoring <jats:italic>Aspergillus<\/jats:italic> infections and epidemiological approaches. In order to identity <jats:italic>Aspergilli<\/jats:italic> species (spp.), a number of molecular methods including restriction fragment length polymorphism (RFLP) have been employed in accordance with ribosomal RNA amplification. The focus of this study \u2014 a group of hospitalized patients with clinical and subclinical signs of infection. All of the collected clinical specimens were transported to the medical mycology lab and examined for <jats:italic>Aspergillus<\/jats:italic> identification. The environmental specimens were collected from air and surfaces inspected for the <jats:italic>Aspergillus<\/jats:italic> within the hospital sources. At first, growth characteristics and microscopic features on mycological media for the identification of <jats:italic>Aspergillus<\/jats:italic> sp. were performed. For the confirmation of <jats:italic>Aspergillus<\/jats:italic> isolates which similarly found in clinical and environmental sources, molecular method polymerase chain reaction\/restriction fragment length polymorphism was carried out. From the mentioned specimens, 102 fungal isolates included <jats:italic>Candida<\/jats:italic> spp., <jats:italic>Aspergillus<\/jats:italic> spp. and other fungi. <jats:italic>Aspergillus flavus<\/jats:italic> (47%), <jats:italic>Aspergillus fumigatus<\/jats:italic> (29.4%) and <jats:italic>Aspergillus niger<\/jats:italic> (23.5%) all were found as the most common clinical isolates. In addition, <jats:italic>Aspergillus<\/jats:italic> isolates from environmental were <jats:italic>Aspergillus niger<\/jats:italic> (43.7%), <jats:italic>Aspergillus flavus<\/jats:italic> (41.7%), <jats:italic>Aspergillus fumigatus<\/jats:italic> (14.6%). Therefore, polymerase chain reaction-restriction fragment length polymorphism with a single restriction enzyme can be very useful in the identification of <jats:italic>Aspergillus<\/jats:italic> spp., because of its facility in use, speed, robust, and high sensitivity of diagnosis.<\/jats:p>","DOI":"10.25122\/jml-2018-0057","type":"journal-article","created":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T12:36:23Z","timestamp":1668688583000},"page":"128-132","source":"Crossref","is-referenced-by-count":15,"title":["Aspergillus diversity in the environments of nosocomial infection cases at a university hospital"],"prefix":"10.25122","volume":"12","author":[{"given":"Kambiz","family":"Diba","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Medical Mycology and Parasitology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Kambiz Diba, Department of Medical Mycology and Parasitology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran, E-mail: kambiz37diba@gmail.com","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farzaneh","family":"Jangi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia, Iran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khadijeh","family":"Makhdoomi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia, Iran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naser","family":"Moshiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Imam Khomeini Hospital, Urmia University of Medical Sciences, Urmia, Iran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatemeh","family":"Mansouri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Genetics and Immunology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10755","published-online":{"date-parts":[[2019,4]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"1. 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J Clin Microbiol. 1995;33(12):3216\u201320.","DOI":"10.1128\/jcm.33.12.3216-3220.1995"}],"container-title":["Journal of Medicine and Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/medandlife.org\/wp-content\/uploads\/JMedLife-12-128.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,17]],"date-time":"2022-11-17T12:36:39Z","timestamp":1668688599000},"score":1,"resource":{"primary":{"URL":"https:\/\/medandlife.org\/wp-content\/uploads\/JMedLife-12-128.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["JMedLife-12-128"],"URL":"https:\/\/doi.org\/10.25122\/jml-2018-0057","relation":{},"ISSN":["1844-122X","1844-3117"],"issn-type":[{"value":"1844-122X","type":"print"},{"value":"1844-3117","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4]]}}}