{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:31:53Z","timestamp":1762432313334},"reference-count":61,"publisher":"S.C. JURNALUL PENTRU MEDICINA SI VIATA S.R.L","issue":"4","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMedLife"],"abstract":"<jats:p>The restoration of voluntary muscle activity in posttraumatic paraplegia in both animal experiments and other clinical applications requires reproducibility of a technically-demanding microsurgical procedure, limited by physicians\u2019 understanding of Brunelli\u2019s spinal cord grafting paradigm. The insufficient clinical investigation of the long-term benefits of the CNS-PNS graft application warrants additional inquiry.<\/jats:p><jats:p>The objective of this study is to explore the potential benefits of the first replicated, graft-induced neuroregeneration of denervated skeletal muscle regarding long-term clinical outcomes and to investigate the effect of Cerebrolysin on neuromodulation.<\/jats:p><jats:p>A randomized study evaluating 30 rats, approved by the National Animal Ethics Advisory Committee was performed. The medication was administered postoperatively. For 14 days, 12 rats received Cerebrolysin (serum), 11 received NaCl 0.9% (shams), and 7 were controls. For microsurgery, the lateral corticospinal tract T10 was grafted to the denervated internal obliquus abdominal muscle. On day 90, intraoperative proof of reinnervation was observed. On day 100, 15 rats were euthanized for fixation, organ removal, and extensive histology-morphology examination, and the Wei-Lachin statistical procedure was employed.<\/jats:p><jats:p>After an open revision of 16 rats, 8 were CMAP positive. After intravenous Vecuronium application, two (Cerebrolysin, NaCl) out of two rats showed an incomplete compound muscle action potential (CMAP) loss due to glutamatergic and cholinergic co-transmission, while two others showed a complete loss of amplitude.<\/jats:p><jats:p>Cerebrolysin medication initiated larger restored muscle fiber diameters and less scarring. FB+ neurons were not observed in the brain but were observed in the Rexed laminae.<\/jats:p><jats:p>Brunelli\u2019s concept was successfully replicated, demonstrating the first graft induced existence of cholinergic and glutamatergic neurotransmission in denervated grafted muscles. Statistics of the histometric count of muscle fibers revealed larger fiber diameters after Cerebrolysin.<\/jats:p><jats:p>Brunelli\u2019s CNS-PNS experimental concept is suitable to analyze graft-neuroplasticity focused on the voluntary restoration of denervated skeletal muscles in spinal cord injury. Neuroprotection by Cerebrolysin is demonstrated.<\/jats:p>","DOI":"10.25122\/jml-2019-0063","type":"journal-article","created":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T05:34:31Z","timestamp":1668576871000},"page":"342-353","source":"Crossref","is-referenced-by-count":2,"title":["Regeneration of Denervated Skeletal Muscles \u2013 Brunelli\u2019s CNS-PNS Paradigm"],"prefix":"10.25122","volume":"12","author":[{"given":"Tobias","family":"von Wild","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Plastic Reconstructive and Aesthetic Surgery, Hand Surgery, Praxisklinik in der Alster City, Hamburg, Germany","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giorgio A.","family":"Brunelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"School of Specialists in Orthopedics, Traumatology, Hand and Microsurgery, University of Brescia, Brescia, Italy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Foundation Giorgio Brunelli for Research on Spinal Cord Lesions ONLUS, E.S.C.R.I., Brescia, Italy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus R.H.","family":"von Wild","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Neurosurgery, Medical Faculty Westphalia Wilhelm\u2019s University M\u00fcnster, M\u00fcnster, Germany","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"International Neuroscience Institute, Hanover, Germany","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Klaus R.H. von Wild, MD, PhD, Prof. of Neurosurgery, Medical Faculty Westphalia Wilhelm\u2019s University M\u00fcnster, Frauenburgstr. 32, 48155 M\u00fcnster, Germany, Prof. of Neurosurgical Neurorehabilitation and Re-Engineering of brain and spinal cord lesions, International Neuroscience Institute, Hanover, Germany; Phone +49-2513977750; cell +491753640400, E-mail: kvw@neurosci.de","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marlene","family":"L\u00f6hnhardt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Plastic and Reconstructive Surgery, Hand Surgery, University Hospital, Hamburg, Germany","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cornel","family":"Catoi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Pathology, University of Agricultural Science and Veterinary Medicine, Cluj-Napoca, Romania","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Florinela Catoi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Functional Biosciences, University of Medicine and Pharmacy \u201cIuliu Hatieganu\u201d, Cluj-Napoca, Romania","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes C.","family":"Vester","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Biometry & Clinical Research, idv Data Analysis and Study Planning, Gauting, Germany","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Strilciuc","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Neurology, University of Medicine and Pharmacy \u201cIuliu Hatieganu\u201d, Cluj-Napoca; Romania","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Trillenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Neurology, University Medical Center Schleswig-Holstein, L\u00fcbeck, Germany","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10755","published-online":{"date-parts":[[2019,10]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Brunelli GA. 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Experimental Cell Research 2014, 234-255","DOI":"10.1016\/j.yexcr.2014.06.020"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Masliah E, D\u00edez-Tejedor E The pharmacology of neurotrophic treatment with Cerebrolysin: brain protection and repair to counteract pathologies of acute and chronic neurological disorders. Drugs Today (Barc) 2012; 48 Suppl A:3-24.","DOI":"10.1358\/dot.2012.48(Suppl.A).1739716"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"Sharma HS: Selected combination of neurotrophins potentials neuroprotection and functional recovery following spinal cord injury in the rat. In: Czernicki Z, Baethmann A, Katayama Y, Kuroiwa T, Mendelow D eds: Brain edema XIV, Heidelberg:Springer Publishers 2010; 358-364","DOI":"10.1007\/978-3-211-98811-4_55"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Muresanu DF: Neuroprotection and neuroplasticity- a holistic approach and future perspectives. J Neurolog Sciences 2007; 257: 38-43","DOI":"10.1016\/j.jns.2007.01.041"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Haninec P, Dubov\u00fd P, S\u00e1mal F, et al. Reinnervation of the rat musculocutaneous nerve stump after its direct reconnection with the C5 spinal cord segment by the nerve graft following avulsion of the ventral spinal roots: a comparison of intrathecal administration of brain-derived neurotrophic factor and Cerebrolysin. Exp Brain Res 2004, 159(4):425-32","DOI":"10.1007\/s00221-004-1969-z"},{"key":"ref19","unstructured":"European Commission Legislation for the protection of animals used for scientific purposes 2010 directive 2010\/63\/EU http:\/\/ec.europa.eu\/environment\/chemicals\/lab_ animals\/index_en.htm"},{"key":"ref20","unstructured":"NIH guide for the care and use of laboratory animals. NIH Publication National Academy of Sciences. The National Academy Press, Washington D.C., USA, 2010"},{"key":"ref21","unstructured":"Slatter DH (ed) (2003) Veterinarian surgery. Textbook of small animal surgery 3 rd ed. USA, Vol. 1, Philadelphia; Sounders Elsevier Science, 2010"},{"key":"ref22","unstructured":"Waynforth HB, Flecknel P. A Experimental and surgical technique in the rat. 2nd ed. Boston, Heidelberg, London: Academic Press San Diego - Amsterdam, UK Elsevier academic press 1992; 100-152, 313"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Watson C., Paxino, G., Kayalioglu, et al. Atlas of the Rat Spinal Cord. In: Watson C, Paxino, G, Kayalioglu, G eds The spinal cord. 1 st Edition. A Christopher and Dana Reeve Foundation text and Atlas. Academic Press 2008; 238-306","DOI":"10.1016\/B978-0-12-374247-6.50019-5"},{"key":"ref24","unstructured":"IACUC Guidelines: Anesthesia Office of animal resources institutional animal care and use university of Iowa.(2016 https:\/\/animal.research.uiowa.edu\/iacuc- guidelines-anesthesia"},{"key":"ref25","unstructured":"Johns Hopkins University (JHU) (2006) Animal care and use policies and guidelines. Use of Ether for animal anesthesia at Johns Hopkins University (Revised by the JHU Joint Health Safety and Environment\/Animal Care and Use committee) 2006; http:\/\/web.jhu.edu\/animalcare\/contact.html"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Smith DC An evoked compound electromyogram simulator with external microprocessor control facility. J Med Eng Technol 1992; 16: 129-32.","DOI":"10.3109\/03091909209021975"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"Kuntzer T, Flocard F, Vial C, et al. Exercise test in muscle channel neuropathies and other muscle disorders. Muscle Nerve 2000; 23:1089-1094","DOI":"10.1002\/1097-4598(200007)23:7<1089::AID-MUS12>3.0.CO;2-Q"},{"key":"ref28","doi-asserted-by":"crossref","unstructured":"Wie LJ, Lachin JM (1984) Two-sample asymptotically distribution-free tests for incomplete multi-variate observations. J Am Stat Assoc 79: 653-661.","DOI":"10.1080\/01621459.1984.10478093"},{"key":"ref29","doi-asserted-by":"crossref","unstructured":"Lachin JM Some large-sample distribution-free estimators and tests for multivariate partially incomplete data from two populations. Stat. Med. 1992; 11: 1151-1170","DOI":"10.1002\/sim.4780110903"},{"key":"ref30","unstructured":"Rahlfs VW, Vester J Der multidimensionale Ansatz statt Testung einzelner Endpunkte. Pharm Med 2012 14: 160-165"},{"key":"ref31","doi-asserted-by":"crossref","unstructured":"Rexed B The cytoarchitectonic organization of the spinal cord in the cat. J Comp Neurol 1952; 96: 414\u2013495","DOI":"10.1002\/cne.900960303"},{"key":"ref32","doi-asserted-by":"crossref","unstructured":"Goldberg JL How does an axon grow? Genes Dev 2003; 17: 941-958","DOI":"10.1101\/gad.1062303"},{"key":"ref33","doi-asserted-by":"crossref","unstructured":"Brumovsky PR VGLUTs in peripheral neurons and the spinal cord: time for a review. ISRN Neurol 2013; 1: 829753. Doi:10.1155\/2013\/829753","DOI":"10.1155\/2013\/829753"},{"key":"ref34","doi-asserted-by":"crossref","unstructured":"Darian-Smith C. Synaptic plasticity, neurogenesis, and functional recovery after spinal cord injury. Neuroscientist 2009;15: 149-165","DOI":"10.1177\/1073858408331372"},{"key":"ref35","doi-asserted-by":"crossref","unstructured":"Weidner N, Ner A, Salimi N, et al. Spontaneous corticospinal axonal plasticity and functional recovery after adult central nervous system injury. PNAS 2001; 98: 35133518","DOI":"10.1073\/pnas.051626798"},{"key":"ref36","doi-asserted-by":"crossref","unstructured":"Beattie MS, Li Q, Bresnahan JC Cell death and plasticity after experimental spinal cord injury. Prog Brain Res 2000; 128: 9\u201321","DOI":"10.1016\/S0079-6123(00)28003-5"},{"key":"ref37","doi-asserted-by":"crossref","unstructured":"Horvat JC, Pecot-Dechavassine M, Mira JC, et al. Formation of functional endplates by spinal axons regenerating through a peripheral nerve graft. A study in the adult rat. Brain Res Bull 1989; 22:103\u201314","DOI":"10.1016\/0361-9230(89)90134-2"},{"key":"ref38","doi-asserted-by":"crossref","unstructured":"Kundi S, Bicknell, R, Ahmed, Z The role of angiogenic and wound-healing factors after spinal cord injury in mammals. Neurosci Res 2013; 76: 1-9","DOI":"10.1016\/j.neures.2013.03.013"},{"key":"ref39","doi-asserted-by":"crossref","unstructured":"Ribeiro-Resende VT, Koenig B, Nichterwitz S, et al. Strategies for inducing the formation of bands of B\u00fcngner in peripheral nerve regeneration. Biomaterials 2009;30: 5251-5259.","DOI":"10.1016\/j.biomaterials.2009.07.007"},{"key":"ref40","doi-asserted-by":"crossref","unstructured":"Francolini M, Brunelli G, Cambianica I, et al. Glutamatergic reinnervation and assembly of glutamatergic synapses in adult rat skeletal muscle occurs at cholinergic endplates. J Neuropathol Exp Neurol 2009; 68: 1103-1115","DOI":"10.1097\/NEN.0b013e3181b7bfc8"},{"key":"ref41","doi-asserted-by":"crossref","unstructured":"Schwab ME, Strittmatter SM Nogo limits neural plasticity and recovery from injury. Curr Opin Neurobiol 2014; 27: 53-60","DOI":"10.1016\/j.conb.2014.02.011"},{"key":"ref42","doi-asserted-by":"crossref","unstructured":"Kadoya K, Nguyen K, Lee-Kubli C, et al. Spinal cord resconstituation with homologous neural grafts enables robust corticospinal regeneration. Nat.Med 2016; 22: 479-487","DOI":"10.1038\/nm.4066"},{"key":"ref43","doi-asserted-by":"crossref","unstructured":"Papastefanski F, Matsas R From demyelination to remyelination: the road toward therapies for spinal cord injry. Glia 2015: 63: 1101-25. doi: 10.1002\/glia.22809. Epub 2015 Mar 2","DOI":"10.1002\/glia.22809"},{"key":"ref44","doi-asserted-by":"crossref","unstructured":"Filli L, Engmann AK, Z\u00f6rner B, et al. Bridging the gap: a reticulo-propriospinal detour bypassing an incomplete spinal cord injury. J Neurosci. 2014; 34: 13399- 62.","DOI":"10.1523\/JNEUROSCI.0701-14.2014"},{"key":"ref45","doi-asserted-by":"crossref","unstructured":"Carlstedt T New treatments for spinal nerve root avulsion injury. Front Neurol 2016; 7: 135. doi: 10.3389\/fneur.2016.00135","DOI":"10.3389\/fneur.2016.00135"},{"key":"ref46","doi-asserted-by":"crossref","unstructured":"Athanasiou A , Klados M A, Pandria N, et al: A Systematic Review of investigations into functional brain connectivity following spinal cord Injury. Front Hum Neurosci. 2017; : 517.published online 2017 Oct 25. doi: [10.3389\/fnhum.2017.00517] PMCID: PMC5669283 PMID: 29163098","DOI":"10.3389\/fnhum.2017.00517"},{"key":"ref47","doi-asserted-by":"crossref","unstructured":"Nardone R, H\u00f6ller Y, Brigo F et al. Functional brain reorganization after spinal cord injury: systematic review of animal and human studies. Brain Res. 2013 Apr 4;1504:58-73. doi: 10.1016\/j.brainres.2012.12.034. Epub 2013 Feb 8.","DOI":"10.1016\/j.brainres.2012.12.034"},{"key":"ref48","unstructured":"Yuying Chen Y, Susan Charlifue S, Noonan V et al: International Spinal Cord Injury Socio-Demographic Basic Data Set (Version 1.0) 201808.10"},{"key":"ref49","doi-asserted-by":"crossref","unstructured":"8 Young W Spinal cord regeneration. Cell Transplant 2014; 23: 573-611","DOI":"10.3727\/096368914X678427"},{"key":"ref50","doi-asserted-by":"crossref","unstructured":"Cramer SC, Sur M, Dobkin BH, et al. 2011; Harnessing neuroplasticity for clinical application. Brain 134: 1591\u20131609","DOI":"10.1093\/brain\/awr039"},{"key":"ref51","doi-asserted-by":"crossref","unstructured":"SK O., SR J. Current Concept of Stem Cell Therapy for Spinal Cord Injury: A Review. Korean J Neurotrauma. 2016;12:40-46. Epub 2016 Oct 31.","DOI":"10.13004\/kjnt.2016.12.2.40"},{"key":"ref52","doi-asserted-by":"crossref","unstructured":"Stoltz JF, de Isla N, Li YP et al. Stem Cells and Regenerative Medicine: Myth or Reality of the 21th Century. Stem Cells Int 2015; 34731: 734-731","DOI":"10.1155\/2015\/734731"},{"key":"ref53","doi-asserted-by":"crossref","unstructured":"Dobkin BH What matters in cellular transplantation for spinal cord injury: the cells, the rehabilitation, or the best mix? Neurorehabil Neural Repair 2010; 24: 7-9","DOI":"10.1177\/1545968309354457"},{"key":"ref54","doi-asserted-by":"crossref","unstructured":"Yilmaz T, Kaptanoglu E. Current and future medical therapeutic strategies for functional repair of spinal cord injury World J Orthop 2015; 6: 42-55","DOI":"10.5312\/wjo.v6.i1.42"},{"key":"ref55","unstructured":"Doucet BM, Lam A, Griffin L. Neuromuscular electrical stimulation for skeletal muscle function. Yale J Biol Med. 2012; 85):201-15. Epub 2012 Jun 25."},{"key":"ref56","doi-asserted-by":"crossref","unstructured":"Von Wild K, Rabischong P, Brunelli G, et al. Computer added locomotion by implanted electrical stimulation in paraplegic patients (SUAW). Acta Neurochir 2008; 79: 99-104.","DOI":"10.1007\/978-3-7091-6105-0_22"},{"key":"ref57","doi-asserted-by":"crossref","unstructured":"Wells JW, Hurlbert RJ, Fehlings MG, et al Neuroprotection by minocycline facilitates significant recovery from spinal cord injury in mice. Brain 2003; 126: 1628-1637","DOI":"10.1093\/brain\/awg178"},{"key":"ref58","doi-asserted-by":"crossref","unstructured":"Muresanu DF, Heiss WD, Hoemberg V, et al. Cerebrolysin and recovery after stroke (CARS): A randomized, placebo-controlled, double-blind, multicenter trial. Stroke 2016: 47: 151-159","DOI":"10.1161\/STROKEAHA.115.009416"},{"key":"ref59","doi-asserted-by":"crossref","unstructured":"Keilhoff G, Lucas B, Josephine Pinkernelle Show J et al. Effects of cerebrolysin on motor-neuron-like NSC-34 cells. Exp Cell Res. 2014; 1;327(2):234-55. doi: 10.1016\/j.yexcr.2014.06.020. 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