{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T16:59:56Z","timestamp":1763398796539,"version":"build-2065373602"},"reference-count":22,"publisher":"S.C. JURNALUL PENTRU MEDICINA SI VIATA S.R.L","issue":"2","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMedLife"],"abstract":"<jats:p>Mesenchymal Stem Cells (MSCs) under TNF-\u03b1 stimulation (MSC-CM-T) can release numerous trophic and survival molecules that have a promising prospect in wound healing acceleration. However, the effective levels of MSC-CM-T in topical gel preparation to accelerate wound healing should be further explored. The aim of this study was to investigate the effects of MSC-CM-T in topical gel preparation in accelerating optimal wound healing through analyzing PDGF levels, wound closure rate percentages, and fibroblast density appearances. Twenty-four male Wistar rats were performed a full-thickness excision. The group studies were randomly assigned into four subgroups: control gel, control medium, and two treatment groups: MSC-CM-T topical gel at doses of 100 \u03bcL and 200 \u03bcL (T1 and T2, respectively). Wound closure rates were measured by standard caliper, platelet-derived growth factor (PDGF) levels were analyzed using ELISA on days 3 and 6, whereas the fibroblast density appearances were determined using hematoxylin-eosin staining. This study found a significant increase in PDGF levels in all treatment groups on days 3 and 6. These findings were in line with the increase of wound closure rates in all treatment groups on day 6, in which the high dose of MSC-CM-T was more effective in initiating the increase of wound closure rate. We also found the fibroblast density appearances on day 6 in the T2 group. We conclude that the topical gel of MSC-CM-T is more effective in accelerating wound closure healing through increasing PDGF levels and wound closure percentages and fibroblast density appearances in the skin defect animal models.<\/jats:p>","DOI":"10.25122\/jml-2019-0103","type":"journal-article","created":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T16:44:31Z","timestamp":1668789871000},"page":"214-221","source":"Crossref","is-referenced-by-count":11,"title":["Topical gel of mesenchymal stem cells-conditioned medium under TNF-\u03b1 precondition accelerates wound closure healing in full-thickness skin defect animal model"],"prefix":"10.25122","volume":"15","author":[{"given":"Agung","family":"Putra","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Stem Cell and Cancer Research (SCCR), Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Postgraduate Biomedical Science, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Pathological Anatomy, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Agung Putra, Stem Cell and Cancer Research (SCCR) Laboratory, Sultan Agung Islamic University, Semarang, Indonesia. E-mail: dr.agungptr@gmail.com","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sugeng","family":"Ibrahim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Molecular Biology and Biochemistry, Faculty of Medicine, Universitas Katolik Soegijapranata, Semarang, Indonesia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Doctoral Program of Medical and Health Science, Faculty of Medicine, Diponegoro University, Semarang, Indonesia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adi Muradi","family":"Muhar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Surgery, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Novalia","family":"Kuntardjo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Student of Postgraduate Biomedical Science Program, Faculty of Medicine, Sultan Agung Islamic University, Semarang, Indonesia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bayu Tirta","family":"Dirja","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Microbiology, Faculty of Medicine, Mataram University, Mataram, Indonesia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zenitalia","family":"Pasongka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Postgraduate Biomedical Science Program, Faculty of Medicine, Udayana University, Bukit Jimbaran, Indonesia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Insan Sosiawan","family":"Tunru","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Pathological Anatomy, Faculty of Medicine, YARSI University, Jakarta, Indonesia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10755","published-online":{"date-parts":[[2022,2]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"1. 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