{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T04:59:48Z","timestamp":1770267588098,"version":"3.49.0"},"reference-count":25,"publisher":"S.C. JURNALUL PENTRU MEDICINA SI VIATA S.R.L","issue":"1","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMedLife"],"abstract":"<jats:p>Diabetes mellitus (DM) is a metabolic disorder that results from insufficient secretion or insulin resistance, or both. Insulin secretion deficiency leads to chronic hyperglycemia along with impaired metabolism of proteins, lipids, and carbohydrates. This study aimed to investigate the TP53 gene SNP (single nucleotide polymorphism) rs1042522 genotype and the interleukin-6 (IL-6) gene SNP rs1800795 genotype in DM and control groups. This study was performed on 70 patients with type 1 DM, 100 patients with type 2 DM without related complications, 66 control subjects for type 1 DM, and 95 control subjects for type 2 DM. The control groups were matched regarding age and gender and did not have a familial relationship with the patient groups. All the subjects were residents of Kermanshah, located in the western part of Iran. Polymorphisms of TP53 and IL-6 genes were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Lipid profile, fasting blood glucose, and HbA1c were measured using the ELISA and immunoturbidometric methods. The frequency of genotypes (CC, CG, GG) of the TP53 gene codon 72 in type 1 DM and its control group were significantly different (P= 0.013). Likewise, the frequency of genotypes (CC, CG, GG) of the TP53 gene codon 72 was significantly different between type 2 DM and control groups (P &lt;0.001). The frequency of genotypes (GG, GC, CC) of G174C polymorphisms in the IL-6 gene was different between type 1 DM and control group as well as between type 2 DM and its control group, but it was not statistically significant. SNP rs1042522 genotypes in the dominant form (CG + GG vs. CC) (OR= 3.880; P &lt; 0.001) and alleles G vs. C alleles (OR= 0.384; P &lt; 0.001) increased the risk of type 2 DM significantly. There was no significant difference between type 1 and type 2 DM groups and respected control groups regarding the frequency of the IL-6 gene SNP rs1800795 alleles. The G allele of SNP rs1042522 encoding the TP53 gene increases the risk of developing DM in the population of the Kermanshah province, Iran.<\/jats:p>","DOI":"10.25122\/jml-2019-0150","type":"journal-article","created":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T08:29:50Z","timestamp":1618561790000},"page":"37-44","source":"Crossref","is-referenced-by-count":14,"title":["Relationship between TP53 and interleukin-6 gene variants and the risk of types 1 and 2 diabetes mellitus development in the Kermanshah province"],"prefix":"10.25122","volume":"14","author":[{"given":"Lida","family":"Haghnazari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Clinical Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"* Corresponding Author: Lida Haghnazari, Department of Clinical Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran. Phone: 0098918833873. E-mail: Lida_haghnazari@yahoo.com","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramin","family":"Sabzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10755","published-online":{"date-parts":[[2021,1]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"1. Ghavimi R, Sharifi M, Mohaghegh MA, Mohammadian H, Khadempar S, Rezaei H. Lack of association between rs1800795 (-174 G\/C) polymorphism in the promoter region of interleukin-6 gene and susceptibility to type 2 diabetesin Isfahan population. Advanced biomedical research. 2016;5.","DOI":"10.4103\/2277-9175.175904"},{"key":"ref2","unstructured":"2. Kasper B, Fauci, Longo, Jameson, Hauser. Harrison's Principles of internal medicine- 16th Ed.-New York: McGraw-Hill Medical Publishing Division, 2005."},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"3. Murray S. Doubling the burden: chronic disease. Canadian Medical Association Journal, 2006;174(6):771.","DOI":"10.1503\/cmaj.060161"},{"key":"ref4","unstructured":"4. Azizi F. Epidemiology of Diabetes Mellitus.2nd International Seminar of Diabetes up Date 2003; 1\u20137."},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"5. Tuomilehto J, Virtala E, Karvonen M, Lounamaa R, PITK\u0100NIEMI J, Reunanen A, et al. Increase in incidence of insulin-dependent diabetes mellitus among children in Finland. International journal of epidemiology 1995;24(5):984\u201392.","DOI":"10.1093\/ije\/24.5.984"},{"key":"ref6","unstructured":"6. Mohan V, Sandeep S, Deepa R, Shah B, Varghese C. Epidemiology of type 2 diabetes: Indian scenario. Indian journal of medical research 2007;125(3):217."},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"7. King H, Aubert RE, Herman WH. Global burden of diabetes, 1995\u20132025: prevalence, numerical estimates, and projections. Diabetes care 1998;21(9):1414\u201331.","DOI":"10.2337\/diacare.21.9.1414"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"8. Alarcon-Aguilar FJ, Roman-Ramos R, Jimenez-Estrada M, Reyes-Chilpa R, Gonzalez-ParedesB, Flores-Saenz JL. Effects of three Mexican medicinal plants (Asteraceae) on blood glucose levels in healthy mice andrabbits. Journal of Ethnopharmacology 1997 ;55(3):171\u20137.","DOI":"10.1016\/S0378-8741(96)01494-8"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"9. Kahn S. The relative contributions of insulin resistance and beta-cell dysfunction to the pathophysiology of type 2 diabetes. Diabetologia 2003 ;46(1):3\u201319.","DOI":"10.1007\/s00125-002-1009-0"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"10. Pim D, Banks L. p53 polymorphic variants at codon 72 exert different effects on cell cycle progression. International journal of cancer 2004 ;108(2):196\u20139.","DOI":"10.1002\/ijc.11548"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"11. Kung C-P, Murphy ME. The role of the p53 tumor suppressor in metabolism and diabetes. Journal of Endocrinology. 2016;231(2): R61\u2013R75.","DOI":"10.1530\/JOE-16-0324"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"12. Piskacek S, Gregor M, Nemethova M, Grabner M, Kovarik P, Piskacek M.Nine-amino-acid transactivation domain: establishment and prediction utilities. Genomics 2007;89(6):756\u201368.","DOI":"10.1016\/j.ygeno.2007.02.003"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"13. Cui R, Widlund HR, Feige E, Lin JY, Wilensky DL, Igras VE, et al. Central role of p53 in the suntan response and pathologic hyperpigmentation. Cell. 2007;128(5):853\u201364.","DOI":"10.1016\/j.cell.2006.12.045"},{"key":"ref14","unstructured":"14. Sanchooli T, Heidari Z, Mahmoudzadeh-Sagheb H, Hashemi M, Rigi-Ladez M. The Relationship between Interleukin-6-174 G\/C Gene polymorphism and chronic periodontitis. ZJRMS 2012, 14(3): 13\u201317"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"15. Testa R, Olivieri F, Bonfigli A, Sirolla C, Boemi M, Marchegiani F, et al. Interleukin-6\u2013174 G> C polymorphism affects the association between IL-6 plasma levels and insulin resistance in type 2 diabetic patients. Diabetes research and clinical practice 2006;71(3):299\u2013305.","DOI":"10.1016\/j.diabres.2005.07.007"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"16. Manca Bitti M, Saccucci P, Bottini E, Gloria-Bottini F. p53 codon 72 polymorphism and type 1 diabetes mellitus. Journal of Pediatric Endocrinology and Metabolism 2010;23(3):291\u20132.","DOI":"10.1515\/JPEM.2010.23.3.291"},{"key":"ref17","unstructured":"17. Spitsina E IN, Chudakova D, Nikitin A, Svetlova G, Soluianova T, et al. Association of polymorphous markers Pro72Arg and C (-594) CC OF TP53 gene with diabetic polyneuropathy in patients with type 1 diabetes mellitus living in Moscow. Molekuliarnaia biologiia. 2006;41(6):989\u201393."},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"18. Qu L, He B, Pan Y, Xu Y, Zhu C, Tang Z, et al. Association between polymorphisms in RAPGEF1, TP53, NRF1 and type 2 diabetes in Chinese Han population. Diabetes research and clinical practice. 2011;91(2):171\u20136.","DOI":"10.1016\/j.diabres.2010.11.019"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"19. Burgdorf KS, Grarup N, Justesen JM, Harder MN, Witte DR, J\u00f8rgensen T, et al. Studies of the association of Arg72Pro of tumor suppressor protein p53 with type 2 diabetes in a combined analysis of 55,521 Europeans. PLoS one. 2011;6(1):e15813.","DOI":"10.1371\/journal.pone.0015813"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"20. Kung C-P, Julia I, Leu J, Basu S, Khaku S, Anokye-Danso F, et al. The P72R polymorphism of p53 predisposes to obesity and metabolic dysfunction. Cell reports. 2016;14(10):2413\u201325.","DOI":"10.1016\/j.celrep.2016.02.037"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"21. Gloria-Bottini F, Banci M, Saccucci P, Magrini A, Bottini E. Is there a role of p53 codon 72 polymorphism in the susceptibility totype 2 diabetes in overweight subjects? A study in patients with cardiovascular diseases. Diabetes research and clinical practice. 2011;91(3):e64-e7.","DOI":"10.1016\/j.diabres.2010.11.031"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"22. Testa R, Olivieri F, Bonfigli A, Sirolla C, Boemi M, Marchegiani F, et al. Interleukin-6\u2013174 G> C polymorphism affects the association between IL-6 plasma levels and insulin resistance in type 2 diabetic patients. Diabetes research and clinical practice. 2006;71(3):299\u2013305.","DOI":"10.1016\/j.diabres.2005.07.007"},{"key":"ref23","unstructured":"23. Rostami F, Hosseini H, Sharifi K, Daneshpour M, Azizi F, Hedayati M. Association of G-174C polymorphism of the interleukin-6 Gene promoter with obesity in Iranian population. World Academy of Science, Engineering and Technology, International Journal of Medical, Health, Biomedical, Bioengineering and Pharmaceutical Engineering. 2010;4(9):400\u20133."},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"24. Huth C, Heid IM, Vollmert C, Gieger C, Grallert H, Wolford JK, et al. IL6 Gene Promoter Polymorphisms and Type 2 Diabetes. Diabetes. 2006;55(10):2915\u201321.","DOI":"10.2337\/db06-0600"},{"key":"ref25","unstructured":"25. Saxena M, Agrawal C, Srivastava N, Banerjee M. Interleukin-6 (IL-6)-597 A\/G (rs1800797) &-174 G\/C (rs1800795) gene polymorphisms in type 2 diabetes. The Indian journal of medical research. 2014;140(1):60."}],"container-title":["Journal of Medicine and Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/medandlife.org\/wp-content\/uploads\/7.-jml-2019-0150.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,16]],"date-time":"2021-04-16T08:30:36Z","timestamp":1618561836000},"score":1,"resource":{"primary":{"URL":"https:\/\/medandlife.org\/wp-content\/uploads\/7.-jml-2019-0150.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["JMedLife-14-37"],"URL":"https:\/\/doi.org\/10.25122\/jml-2019-0150","relation":{},"ISSN":["1844-122X","1844-3117"],"issn-type":[{"value":"1844-122X","type":"print"},{"value":"1844-3117","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1]]}}}