{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T10:52:14Z","timestamp":1793789534122,"version":"4.6.1"},"reference-count":17,"publisher":"S.C. JURNALUL PENTRU MEDICINA SI VIATA S.R.L","issue":"4","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMedLife"],"abstract":"<jats:p>The associations between periodontitis and cardiovascular diseases have been intensely studied in recent years. Oxidative stress is involved in the initiation and both progression of periodontitis and atherosclerosis. Antioxidants can reduce the effects of oxidative stress on inflammatory diseases. Our aim was to measure the effects of a grape seed extract (GSE), rich in antioxidants, on atherosclerosis caused by ligature-induced periodontitis in rats. Thirty male Wistar rats were randomly divided into three groups of 10: control group, periodontitis group, and periodontitis group treated with GSE (GSE group). Periodontitis was induced by placing an orthodontic wire around the cervix of the first mandibular molar and keeping it in place for 4 weeks. On days 1, 7 and 28, blood samples were taken to assess oxidative stress and inflammation markers (malondialdehyde and glutathione - MDA, reduced glutathione - GSH, C reactive protein) and lipids. After 4 weeks, the animals were euthanized, and aortas were collected for histopathologic examination. MDA was significantly higher in Periodontitis group compared to the other groups only at day 7. GSH was significantly increased in the Control and GSE groups on days 1 and 7, compared to Periodontitis group and on day 28 higher in GSE vs. Periodontitis groups. C reactive protein was significantly increased in the Periodontitis group on days 1 and 7 compared to both groups. Cholesterol was significantly decreased in the aortas of GSE group at day 28 compared to the Periodontitis group. Oral administration of a grape seed extract reduces the oxidative stress, inflammation and atherosclerosis in a rat model of ligature-induced periodontitis.<\/jats:p>","DOI":"10.25122\/jml-2020-0177","type":"journal-article","created":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T08:35:58Z","timestamp":1668760558000},"page":"580-586","source":"Crossref","is-referenced-by-count":9,"title":["Grape Seed Extract Reduces the Degree of Atherosclerosis in Ligature-Induced Periodontitis in Rats \u2013 An Experimental Study"],"prefix":"10.25122","volume":"13","author":[{"given":"Tudor","family":"Dimitriu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral and Maxillofacial Surgery, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, Cluj-Napoca, Romania","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pompei","family":"Bolfa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Pathology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Biomedical Sciences, Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and Nevis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soimita","family":"Suciu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Physiology, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, Cluj-Napoca, Romania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Cimpean","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Pathology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zsofia","family":"Daradics","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Pathology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornel","family":"Catoi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Pathology, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Armencea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral and Maxillofacial Surgery, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, Cluj-Napoca, Romania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Gabriel Armencea, Department of Oral and Maxillofacial Surgery, Iuliu Hatieganu University of Medicine and Pharmacy,8 V. Babes, 400012, Cluj-Napoca, Romania E-mail: garmencea@gmail.com","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grigore","family":"Baciut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral and Maxillofacial Surgery, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, Cluj-Napoca, Romania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simion","family":"Bran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral and Maxillofacial Surgery, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, Cluj-Napoca, Romania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristian","family":"Dinu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral and Maxillofacial Surgery, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, Cluj-Napoca, Romania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Cristian Dinu, Department of Oral and Maxillofacial Surgery, Iuliu Hatieganu University of Medicine and Pharmacy,8 V. Babes, 400012, Cluj-Napoca, Romania Phone: +40723352189 E-mail: dinu_christian@yahoo.com","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihaela","family":"Baciut","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Oral and Maxillofacial Surgery, \u201cIuliu Hatieganu\u201d University of Medicine and Pharmacy, Cluj-Napoca, Romania","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10755","published-online":{"date-parts":[[2020,10]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"1. Pihlstrom BL, Michalowicz BS, Johnson NW. Periodontal diseases. Lancet 366, 1809\u20131820 (2005).","DOI":"10.1016\/S0140-6736(05)67728-8"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"2. Janket SJ, Baird AE, Chuang SK, Jones JA. Meta-analysis of periodontal disease and risk of coronary heart disease and stroke. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;95:559\u201369.","DOI":"10.1067\/moe.2003.107"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"3. Chistiakov DA, Orekhov AN, Bobryshev YV. Links between atherosclerotic and periodontal disease. Exp Mol Pathol. 2016 Feb;100(1):220-35.","DOI":"10.1016\/j.yexmp.2016.01.006"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"4. Cairo F, Castellani S, Gori AM, Nieri M, Baldelli G, Abbate R, et al. Severe periodontitis in young adults is associated with sub-clinical atherosclerosis. J Clin Periodontol 2008;35: 465\u201372.","DOI":"10.1111\/j.1600-051X.2008.01228.x"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"5. Kumar KR, Ranganath V, Naik R, Banu S, Nichani AS. Assement of high-sensitivity C-reactive protein and lipid levels in healthy adults and pacients with coronary artery disease, with and without periodontitis \u2013 a cross-sectional study. Journal of Periodontal Research, 2014.","DOI":"10.1111\/jre.12172"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"6. Venugopal S, Devaraj S, Jialal I. Macrophage conditioned medium induces the expression of C-reactive protein in human aortic endothelial cells: potential for paracrine\/autocrine effects. Am J Pathol 2005;166:1265-1271.","DOI":"10.1016\/S0002-9440(10)62345-0"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"7. Kurita-Ochiai T, Jia R, Cai Y, Yamaguchi Y, Yamamoto M. Periodontal Disease-Induced Atherosclerosis and Oxidative Stress. Antioxidants (Basel). 2015 Sep 2;4(3):577-90.","DOI":"10.3390\/antiox4030577"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"8. Budzy\u0144ski J, Wi\u015bniewska J, Ciecierski M, K\u0119dzia A. Int J Angiol. Association between Bacterial Infection and Peripheral Vascular Disease: A Review. 2016 Mar;25(1):3-13.","DOI":"10.1055\/s-0035-1547385"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"9. D Ekuni, Tomofuji T, Sanbe T, Irie K, Azuma T, Maruyama T, Tamaki N, Murakami J, Kokeguchi S, Yamamoto T. Periodontitis-induced lipid peroxidation in rat descending aorta is involved in the initiation of atherosclerosis. J Periodontal Res. 2009 Aug;44(4):434-42.","DOI":"10.1111\/j.1600-0765.2008.01122.x"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"10. Ekuni D, Tomofuji T, Sanbe T, Irie K, Azuma T, Maruyama T, Naofumi T, Murakami J, Kokeguchi S, Yamamoto T. Vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress. Arch Oral Biol. 2009 May;54(5):495-502.","DOI":"10.1016\/j.archoralbio.2009.02.006"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"11. Villacorta L, Azzi A, Zingg JM. Regulatory role of vitamins E and C on extracellular matrix components of the vascular system. Mol Aspects Med 2007;28:507\u201337.","DOI":"10.1016\/j.mam.2007.05.002"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"12. Tomofuji T, Ekuni D, Sanbe T, Irie K, Azuma T, Maruyama T, Tamaki N, Murakami J, Kokeguch S, Yamamoto T. Effects of vitamin C intake on gingival oxidative stress in rat periodontitis. Free Radic Biol Med. 2009 Jan 15;46(2):163-8.","DOI":"10.1016\/j.freeradbiomed.2008.09.040"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"13. Ara\u00fajo AA, Pereira ASBF, Medeiros CACX, Brito GAC, Leit\u00e3o RFC, Ara\u00fajo LS, Guedes PMM, Hiyari S, Pirih FQ, de Ara\u00fajo J\u00fanior RF . Effects of metformin on inflammation, oxidative stress, and bone loss in a rat model of periodontitis. PLoS One. 2017 Aug 28;12(8):e0183506.","DOI":"10.1371\/journal.pone.0183506"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"14. Tamer L, Sucu N, Polat G, Ercan B, Aytacoglu B, Yucebilgi\u00e7 G, Unl\u00fc A, Dikmengil M, Atik U. Decreased serum total antioxidant status and erythrocyte-reduced glutathione levels are associated with increased serum malondialdehyde in atherosclerotic patients. Arch Med Res 2002;33:257\u201360.","DOI":"10.1016\/S0188-4409(01)00381-2"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"15. Ramesh E, Elanchezhian R, Sakthivel M, Jayakumar T, Senthil Kumar RS, Geraldine P, Thomas PA. Epigallocatechin gallate improves serum lipid profile and erythrocyte and cardiac tissue antioxidant parameters in Wistar rats fed an atherogenic diet. Fundam Clin Pharmacol 2008;22:275\u201384.","DOI":"10.1111\/j.1472-8206.2008.00585.x"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"16. Speer H, D'Cunha NM, Botek M, McKune AJ, Sergi D, Georgousopoulou E, Mellor DD , Naumovski N. The Effects of Dietary Polyphenols on Circulating Cardiovascular Disease Biomarkers and Iron Status: A Systematic Review. Nutr Metab Insights. 2019 Oct 21;12:1178638819882739.","DOI":"10.1177\/1178638819882739"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"17. Iiyama K, Hajra L, Iiyama M, Li H, DiChiara M, Medoff BD, Cybulsky MI. Patterns of vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 expression in rabbit and mouse atherosclerotic lesions and at sites predisposed to lesion formation. Circ Res 85, 199\u2013207 (1999).","DOI":"10.1161\/01.RES.85.2.199"}],"container-title":["Journal of Medicine and Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/medandlife.org\/wp-content\/uploads\/JMedLife-13-580.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,18]],"date-time":"2022-11-18T08:36:13Z","timestamp":1668760573000},"score":1,"resource":{"primary":{"URL":"https:\/\/medandlife.org\/wp-content\/uploads\/JMedLife-13-580.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["JMedLife-13-580"],"URL":"https:\/\/doi.org\/10.25122\/jml-2020-0177","relation":{},"ISSN":["1844-122X","1844-3117"],"issn-type":[{"value":"1844-122X","type":"print"},{"value":"1844-3117","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10]]}}}