{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T20:56:52Z","timestamp":1790024212346,"version":"4.0.1"},"reference-count":30,"publisher":"S.C. JURNALUL PENTRU MEDICINA SI VIATA S.R.L","issue":"2","license":[{"start":{"date-parts":[[2021,3,1]],"date-time":"2021-03-01T00:00:00Z","timestamp":1614556800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMedLife"],"abstract":"<jats:p>The thyroid hormone plays a vital role in the development and maturation of the nervous system not only during prenatal and perinatal age but also in adults. \u201cPeripheral marker hypothesis\u201d revealed that gene expression changes in some regions of the brain are reflected into the peripheral blood lymphocytes. The objective of the study was to investigate changes in the gene expression profile of neuropeptides and their receptors in patients with different forms of thyroid pathology. One hundred fifty-three patients with thyroid pathology were enrolled in the study. They were divided into three groups: group 1 included 16 patients with postoperative hypothyroidism, group 2 included 65 patients with hypothyroidism resulting from autoimmune thyroiditis (AIT), and group 3 included 72 patients with AIT and elevated levels of anti-thyroglobulin (anti-Tg) and anti-thyroid peroxidase (anti-TPO) antibodies in the serum. We used a pathway-specific polymerase chain reaction (PCR) array (RT2 Profiler\u2122 PCR Array Human Neurotrophins &amp; Receptors, QIAGEN, Germany) to identify and verify neuropeptides and receptors pathway-focused gene expression in 12 individuals that were randomly selected from each group using real-time PCR. Our research identified that patients with postoperative hypothyroidism had a considerably increased expression of NPY1R, NTSR1, and NPY4R. The patients with hypothyroidism caused by autoimmune thyroiditis had considerably lower expression of NTSR1, while the expression of NPY1R increased. The mRNA levels of NPY2R and PNOC increased in the patients with elevated levels of autoantibodies anti-Tg and anti-TPO in the serum, and mRNA levels of NPY1R and NTSR1 decreased in this group of patients.<\/jats:p>","DOI":"10.25122\/jml-2020-0183","type":"journal-article","created":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T07:29:42Z","timestamp":1634110182000},"page":"243-249","source":"Crossref","is-referenced-by-count":29,"title":["Analysis of the transcriptional activity of genes of neuropeptides and their receptors in the blood of patients with thyroid pathology"],"prefix":"10.25122","volume":"14","author":[{"given":"Iryna Ivanivna","family":"Kamyshna","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Medical Rehabilitation, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"* Corresponding Author: Iryna Ivanivna Kamyshna, MD, PhD, Assistant Professor, Medical Rehabilitation Department, I. Horbachevsky Ternopil National Medical University, Majdan Voli 1, Ternopil, Ukraine, 46001. Phone: +380954313068 E-mail: Iryna.bilous2017@gmail.com","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larysa Borysivna","family":"Pavlovych","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Clinical Immunology, Allergology and Endocrinology, HSEEU Bukovinian State Medical University, Chernivtsi, Ukraine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vitaliy Antonovych","family":"Maslyanko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Clinical Immunology, Allergology and Endocrinology, HSEEU Bukovinian State Medical University, Chernivtsi, Ukraine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandr Mychailovich","family":"Kamyshnyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Microbiology, Virology, and Immunology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10755","published-online":{"date-parts":[[2021,3]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Calz\u00e0 L, Aloe L, Giardino L. Thyroid hormone-induced plasticity in the adult rat brain. Brain Res Bull. 1997;44(4):549\u201357. doi: 10.1016\/s0361-9230(97)00241-4.","DOI":"10.1016\/S0361-9230(97)00241-4"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Porterfield SP, Hendrich CE. The role of thyroid hormones in prenatal and neonatal neurological development-current perspectives. Endocr Rev. 1993;14(1):94\u2013106. doi: 10.1210\/edrv-14-1-94.","DOI":"10.1210\/er.14.1.94"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Courtin F, Chantoux F, Francon J. Thyroid hormone metabolism by glial cells in primary culture. Mol Cell Endocrinol. 1986;48(2-3):167\u201378. doi: 10.1016\/0303-7207(86)90039-0.","DOI":"10.1016\/0303-7207(86)90039-0"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"H\u00f6kfelt T, Broberger C, Xu ZQ, Sergeyev V, Ubink R, Diez M. Neuropeptides-an overview. Neuropharmacology. 2000;39(8):1337\u201356. doi: 10.1016\/s0028-3908(00)00010-1.","DOI":"10.1016\/S0028-3908(00)00010-1"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Lorente L, Mart\u00edn MM, Almeida T, P\u00e9rez-Cejas A, Ramos L, Argueso M, et al. Serum Levels of Substance P and Mortality in Patients with a Severe Acute Ischemic Stroke. Int J Mol Sci. 2016;17(6):991. doi: 10.3390\/ijms17060991.","DOI":"10.3390\/ijms17060991"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Wilkinson M, Brown RE, editors. An Introduction to Neuroendocrinology 2nd ed. Cambridge University Press; 2015. 496p. Neurotransmitters, p.78\u2013119.","DOI":"10.1017\/CBO9781139045803.006"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Rangel-Gomez M, Meeter M. Neurotransmitters and Novelty: A Systematic Review. J Psychopharmacol. 2016;30(1):3\u201312. doi: 10.1177\/0269881115612238.","DOI":"10.1177\/0269881115612238"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Meriney SD, Fanselow EE, editors. Synaptic Transmission. Elsevier; 2019. 528p. Chapter 19, Neuropeptide Transmitters.p. 421\u201334. doi: 10.1016\/B978-0-12-815320-8.00019-3","DOI":"10.1016\/B978-0-12-815320-8.00019-3"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Bilous II, Korda MM, Krynytska IY, Kamyshnyi AM. Nerve impulse transmission pathway-focused genes expression analysis in patients with primary hypothyroidism and autoimmune thyroiditis. 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The Influence of Metformin to the Transcriptional Activity of the mTOR and FOX3 Genes in Parapancreatic Adipose Tissue of Streptozotocin-Induced Diabetic Rats. Journal of Medicine and Life. 2020;13(1):50\u201355.","DOI":"10.25122\/jml-2020-0029"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Garber JR, Cobin RH, Gharib H, Hennessey JV, Klein I, Mechanick JI, Pessah-Pollack R, Singer PA, Woeber KA. Clinical Practice Guidelines for Hypothyroidism in Adults: Cosponsored by the American Association of Clinical Endocrinologists and the American Thyroid Association. Endocr. Pract, 2012, 18:988\u20131028.","DOI":"10.4158\/EP12280.GL"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Kotkowska A, Sewerynek E, Doma\u0144ska D, Pastuszak-Lewandoska D, Brzezia\u0144ska E. Single nucleotide polymorphisms in the STAT3 gene influence AITD susceptibility, thyroid autoantibody levels, and IL6 and IL17 secretion. Cell Mol Biol Lett. 2015;20(1):88\u2013101. doi: 10.1515\/cmble-2015-0004.","DOI":"10.1515\/cmble-2015-0004"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Roseboom PH, Nanda SA, Fox AS, Oler JA, Shackman AJ, Shelton SE, et al. Neuropeptide Y receptor gene expression in the primate amygdala predicts anxious temperament and brain metabolism. Biol Psychiatry. 2014;76(11):850\u20137. doi: 10.1016\/j.biopsych.2013.11.012.","DOI":"10.1016\/j.biopsych.2013.11.012"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"Calvino C, Imp\u00e9rio GE, Wilieman M, Costa-E-Sousa RH, Souza LL, Trevenzoli IH, et sl. Hypothyroidism Induces Hypophagia Associated with Alterations in Protein Expression of Neuropeptide Y and Proopiomelanocortin in the Arcuate Nucleus, Independently of Hypothalamic Nuclei-Specific Changes in Leptin Signaling. Thyroid. 2016;26(1):134\u201343. doi: 10.1089\/thy.2015.0384.","DOI":"10.1089\/thy.2015.0384"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Petitto JM, Huang Z, McCarthy DB. Molecular cloning of NPY-Y1 receptor cDNA from rat splenic lymphocytes: evidence of low levels of mRNA expression and [125I]NPY binding sites. J Neuroimmunol. 1994;54(1\u20132):81\u20136. doi: 10.1016\/0165-5728(94)90234-8.","DOI":"10.1016\/0165-5728(94)90234-8"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Dimitrijevi\u0107 M, Stanojevi\u0107 S. The intriguing mission of neuropeptide Y in the immune system. Amino Acids. 2013;45(1):41\u201353. doi: 10.1007\/s00726-011-1185-7.","DOI":"10.1007\/s00726-011-1185-7"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Singer K, Morris DL, Oatmen KE, Wang T, DelProposto J, Mergian T, et al. Neuropeptide Y is produced by adipose tissue macrophages and regulates obesity-induced inflammation. PLoS One [Internet]. 2013 [Accessed on Mar 5 2020];8(3):e57929. Available at: https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0057929 doi: 10.1371\/journal.pone.0057929.","DOI":"10.1371\/journal.pone.0057929"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Miti\u0107 K, Stanojevi\u0107 S, Ku\u0161trimovi\u0107 N, Vuji\u0107 V, Dimitrijevi\u0107 M. Neuropeptide Y modulates functions of inflammatory cells in the rat: distinct role for Y1, Y2 and Y5 receptors. Peptides. 2011;32(8):1626-33. doi: 10.1016\/j.peptides.2011.06.007.","DOI":"10.1016\/j.peptides.2011.06.007"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"Fetissov SO, Kopp J, H\u00f6kfelt T. Distribution of NPY receptors in the hypothalamus. Neuropeptides. 2004;38(4):175\u201388. doi: 10.1016\/j.npep.2004.05.009.","DOI":"10.1016\/j.npep.2004.05.009"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Lundell I, Blomqvist AG, Berglund MM, Schober DA, Johnson D, et al. Cloning of a human receptor of the NPY receptor family with high affinity for pancreatic polypeptide and peptide YY. J Biol Chem. 1995;270(49):29123\u20138. doi: 10.1074\/jbc.270.49.29123.","DOI":"10.1074\/jbc.270.49.29123"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Johnson EO, Calogero AE, Konstandi M, Kamilaris TC, La Vignera S, Chrousos GP. Effects of short- and long-duration hypothyroidism on hypothalamic-pituitary-adrenal axis function in rats: in vitro and in situ studies. Endocrine. 2012;42(3):684\u201393. doi: 10.1007\/s12020-012-9714-z.","DOI":"10.1007\/s12020-012-9714-z"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"Tohei A. Studies on the functional relationship between thyroid, adrenal and gonadal hormones. J Reprod Dev. 2004;50(1):9\u201320. doi: 10.1262\/jrd.50.9.","DOI":"10.1262\/jrd.50.9"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"Kamilaris TC, DeBold CR, Johnson EO, Mamalaki E, Listwak SJ, Calogero AE, et al. Effects of short and long duration hypothyroidism and hyperthyroidism on the plasma adrenocorticotropin and corticosterone responses to ovine corticotropin-releasing hormone in rats. Endocrinology. 1991;128(5):2567\u201376. doi: 10.1210\/endo-128-5-2567.","DOI":"10.1210\/endo-128-5-2567"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Shi ZX, Levy A, Lightman SL. Thyroid hormone-mediated regulation of corticotropin-releasing hormone messenger ribonucleic acid in the rat. Endocrinology. 1994;134(3):1577\u201380. doi: 10.1210\/endo.134.3.8119200.","DOI":"10.1210\/endo.134.3.8119200"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"Elde R, Schalling M, Ceccatelli S, Nakanishi S, Hokfelt T. (1990). Localization of neuropeptide receptor mRNA in rat brain: initial observations using probes for neurotensin and substance P receptors. Neurosci. Lett. 120, 134\u2013138.","DOI":"10.1016\/0304-3940(90)90187-E"},{"key":"ref28","doi-asserted-by":"crossref","unstructured":"Stolakis V, Kalafatakis K, Botis J, Zarros A, and Liapi C (2010). The regulatory role of neurotensin on the hypothalamic-anterior pituitary axons: emphasis on the control of thyroid-related functions. Neuropeptides 44, 1\u20137.","DOI":"10.1016\/j.npep.2009.09.005"},{"key":"ref29","doi-asserted-by":"crossref","unstructured":"Wolf, SS, Hyde, TM, Saunders, RC, Herman, MM, Weinberger, DR, and Kleinman, JE. (1995). Autoradiographic characterization of neurotensin receptors in the entorhinal cortex of schizophrenic patients and control subjects. J. Neural Transm. Gen. Sect. 102, 55\u201365.","DOI":"10.1007\/BF01276565"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"Fontaine B, Klarsfeld A, H\u00f6kfelt T, Changeux JP. Calcitonin gene-related peptide, a peptide present in spinal cord motoneurons, increases the number of acetylcholine receptors in primary cultures of chick embryo myotubes. 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