{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T17:00:33Z","timestamp":1764349233937},"reference-count":41,"publisher":"S.C. JURNALUL PENTRU MEDICINA SI VIATA S.R.L","issue":"5","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMedLife"],"DOI":"10.25122\/jml-2021-0065","type":"journal-article","created":{"date-parts":[[2021,12,31]],"date-time":"2021-12-31T09:08:10Z","timestamp":1640941690000},"page":"620-635","source":"Crossref","is-referenced-by-count":7,"title":["A new variant of the human \u03b1-lactalbumin-oleic acid complex as an anticancer agent for chronic myeloid leukemia"],"prefix":"10.25122","volume":"14","author":[{"given":"Vivek","family":"Singh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Biochemistry, King George\u2019s Medical University, Lucknow, Uttar Pradesh, India","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Biochemistry, King George\u2019s Medical University, Lucknow, Uttar Pradesh, India","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Biochemistry, King George\u2019s Medical University, Lucknow, Uttar Pradesh, India","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ranjana","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"*Corresponding Author: Ranjana Singh, Department of Biochemistry, King George\u2019s Medical University, Lucknow, Uttar Pradesh, India-226003. E-mail: ranjanasingh.2509@rediffmail.com; ranjanasingh@kgmcindia.edu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dinesh","family":"Kumar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Centre of Biomedical Research, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abbas","family":"Ali Mahdi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anil","family":"Kumar Tripathi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Clinical Hematology, King George\u2019s Medical University, Lucknow, Uttar Pradesh, India","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10755","published-online":{"date-parts":[[2021,1]]},"reference":[{"key":"ref1","unstructured":"1. Singh V, Singh R, Mahdi AA. in-silico analysis of essential amino acids of Alpha-lactalbumin in different species and its association with regulatory proteins for the normal cellular metabolism for growing newborn. International Journal of Emerging Technologies and Innovative Research. 2019 Mar 6;3:191-05."},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"2. H\u00c5KANssoN A, Zhivotovsky B, Orrenius S, Sabharwal H, Svanborg C. Apoptosis induced by a human milk protein. Proceedings of the National Academy of Sciences. 1995 Aug 15;92(17):8064-8.","DOI":"10.1073\/pnas.92.17.8064"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"3. K\u00f6hler C, H\u00e5kansson A, Svanborg C, Orrenius S, Zhivotovsky B. Protease activation in apoptosis induced by MAL. Experimental cell research. 1999 Jun 15;249(2):260-8.","DOI":"10.1006\/excr.1999.4472"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"4. Green KA, Streuli CH. Apoptosis regulation in the mammary gland. Cellular and Molecular Life Sciences. 2004 Jul 1;61(15):1867-83.","DOI":"10.1007\/s00018-004-3366-y"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"5. Svensson M, Sabharwal H, H\u00e5kansson A, Mossberg AK, Lipniunas P, Leffler H, Svanborg C, Linse S. Molecular characterization of \u03b1\u2013lactalbumin folding variants that induce apoptosis in tumor cells. Journal of Biological Chemistry. 1999 Mar 5;274(10):6388-96.","DOI":"10.1074\/jbc.274.10.6388"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"6. Permyakov SE, Knyazeva EL, Leonteva MV, Fadeev RS, Chekanov AV, Zhadan AP, H\u00e5kansson AP, Akatov VS, Permyakov EA. A novel method for preparation of HAMLET-like protein complexes. Biochimie. 2011 Sep 1;93(9):1495-501.","DOI":"10.1016\/j.biochi.2011.05.002"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"7. Svensson M, H\u00e5kansson A, Mossberg AK, Linse S, Svanborg C. Conversion of \u03b1-lactalbumin to a protein inducing apoptosis. Proceedings of the National Academy of Sciences. 2000 Apr 11;97(8):4221-6.","DOI":"10.1073\/pnas.97.8.4221"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"8. Svanborg C, \u00c5gerstam H, Aronson A, Bjerkvig R, D\u00fcringer C, Fischer W, Gustafsson L, Hallgren O, Leijonhuvud I, Linse S, Mossberg AK. HAMLET kills tumor cells by an apoptosis-like mechanism\u2014cellular, molecular, and therapeutic aspects. Advances in cancer research. 2003 Jan 1;88:1-29.","DOI":"10.1016\/S0065-230X(03)88302-1"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"9. Svensson M, Mossberg AK, Pettersson J, Linse S, Svanborg C. Lipids as cofactors in protein folding: Stereo-specific lipid\u2013protein interactions are required to form HAMLET (human \u03b1-lactalbumin m ade le thal to t umor cells). Protein Science. 2003 Dec;12(12):2805-14.","DOI":"10.1110\/ps.0231103"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"10. Kamijima T, Ohmura A, Sato T, Akimoto K, Itabashi M, Mizuguchi M, Kamiya M, Kikukawa T, Aizawa T, Takahashi M, Kawano K. Heat-treatment method for producing fatty acid-bound alpha-lactalbumin that induces tumor cell death. Biochemical and biophysical research communications. 2008 Nov 7;376(1):211-4.","DOI":"10.1016\/j.bbrc.2008.08.127"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"11. Ba\u00f1uelos S, Muga A. Binding of molten globule-like conformations to lipid bilayers structure of native and partially folded \u03b1-lactalbumin bound to model membranes. Journal of Biological Chemistry. 1995 Dec 15;270(50):29910-5.","DOI":"10.1074\/jbc.270.50.29910"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"12. Halskau \u00d8, Fr\u00f8ystein N\u00c5, Muga A, Mart\u0131nez A. The membrane-bound conformation of \u03b1-lactalbumin studied by NMR-monitored 1H exchange. Journal of molecular biology. 2002 Aug 2;321(1):99-110.","DOI":"10.1016\/S0022-2836(02)00565-X"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"13. Halskau \u00d8, Underhaug J, Fr\u00f8ystein N\u00c5, Mart\u00ednez A. Conformational flexibility of \u03b1-lactalbumin related to its membrane binding capacity. Journal of molecular biology. 2005 Jun 24;349(5):1072-86.","DOI":"10.1016\/j.jmb.2005.04.020"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"14. Chenal A, Vernier G, Savarin P, Bushmarina NA, Geze A, Guillain F, Gillet D, Forge V. Conformational states and thermodynamics of \u03b1-lactalbumin bound to membranes: a case study of the effects of pH, calcium, lipid membrane curvature and charge. Journal of molecular biology. 2005 Jun 17;349(4):890-905.","DOI":"10.1016\/j.jmb.2005.04.036"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"15. Agas\u00f8ster AV, Halskau \u00d8, Fuglebakk E, Fr\u00f8ystein N\u00c5, Muga A, Holmsen H, Mart\u00ednez A. The interaction of peripheral proteins and membranes studied with \u03b1-lactalbumin and phospholipid bilayers of various compositions. Journal of Biological Chemistry. 2003 Jun 13;278(24):21790-7.","DOI":"10.1074\/jbc.M211466200"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"16. Chaudhuri D, Narayan M, Berliner LJ. Conformation-dependent interaction of \u03b1-lactalbumin with model and biological membranes: a spin-label ESR study. The protein journal. 2004 Jan 1;23(1):95-101.","DOI":"10.1023\/B:JOPC.0000016263.50484.e1"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"17. Ptitsyn OB. Molten globule and protein folding. InAdvances in protein chemistry, Vol, 46,1995, Academic Press, 83-229","DOI":"10.1016\/S0065-3233(08)60546-X"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"18. Knyazeva EL, Grishchenko VM, Fadeev RS, Akatov VS, Permyakov SE, Permyakov EA. Who is Mr. HAMLET? Interaction of human \u03b1-lactalbumin with monomeric oleic acid. Biochemistry. 2008 Nov 12;47(49):13127-37.","DOI":"10.1021\/bi801423s"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"19. Gaynon PS, Trigg ME, Heerema NA, Sensel MG, Sather HN, Hammond GD, Bleyer WA. Children\u2019s Cancer Group trials in childhood acute lymphoblastic leukemia: 1983\u20131995. Leukemia. 2000 Dec;14(12):2223.","DOI":"10.1038\/sj.leu.2401939"},{"key":"ref20","unstructured":"20. Altieri DC. Survivin apoptosis: an interloper between cell death and cell proliferation in cancer. Lab invest. 1999;11:1327-33."},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"21. Altieri DC. Survivin and apoptosis control. Advances in cancer research. 2003 Jan 1;88:31-52.","DOI":"10.1016\/S0065-230X(03)88303-3"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"22. Ambrosini G, Adida C, Altieri DC. A novel anti-apoptosis gene, survivin, expressed in cancer and lymphoma. Nature medicine. 1997 Aug;3(8):917.","DOI":"10.1038\/nm0897-917"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"23. Velculescu VE, Madden SL, Zhang L, Lash AE, Yu J, Rago C, Lal A, Wang CJ, Beaudry GA, Ciriello KM, Cook BP. Analysis of human transcriptomes. Nature genetics. 1999 Dec;23(4):387.","DOI":"10.1038\/70487"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"24. Chakravarti A, Zhai GG, Zhang M, Malhotra R, Latham DE, Delaney MA, Robe P, Nestler U, Song Q, Loeffler J. Survivin enhances radiation resistance in primary human glioblastoma cells via caspase-independent mechanisms. Oncogene. 2004 Sep;23(45):7494.","DOI":"10.1038\/sj.onc.1208049"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"25. Lu J, Tan M, Huang WC, Li P, Guo H, Tseng LM, Su XH, Yang WT, Treekitkarnmongkol W, Andreeff M, Symmans F. Mitotic deregulation by survivin in ErbB2-overexpressing breast cancer cells contributes to Taxol resistance. Clinical Cancer Research. 2009 Feb 15;15(4):1326-34.","DOI":"10.1158\/1078-0432.CCR-08-0954"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"26. Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC, Altieri DC. Control of apoptosis and mitotic spindle checkpoint by survivin. Nature. 1998 Dec;396(6711):580.","DOI":"10.1038\/25141"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"27. Lakshminarayanan V, Beno DW, Costa RH, Roebuck KA. Differential regulation of interleukin-8 and intercellular adhesion molecule-1 by H2O2 and tumor necrosis factor-\u03b1 in endothelial and epithelial cells. Journal of Biological Chemistry. 1997 Dec 26;272(52):32910-8.","DOI":"10.1074\/jbc.272.52.32910"},{"key":"ref28","doi-asserted-by":"crossref","unstructured":"28. DeForge LE, Preston AM, Takeuchi E, Kenney J, Boxer LA, Remick DG. Regulation of interleukin 8 gene expression by oxidant stress. Journal of Biological Chemistry. 1993 Dec 5;268(34):25568-76.","DOI":"10.1016\/S0021-9258(19)74429-9"},{"key":"ref29","doi-asserted-by":"crossref","unstructured":"29. DeForge LE, Fantone JC, Kenney JS, Remick DG. Oxygen radical scavengers selectively inhibit interleukin 8 production in human whole blood. The Journal of clinical investigation. 1992 Nov 1;90(5):2123-9.","DOI":"10.1172\/JCI116097"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"30. Massion PP, Linden A, Inoue H, Mathy M, Grattan KM, Nadel JA. Dimethyl sulfoxide decreases interleukin-8-mediated neutrophil recruitment in the airways. American Journal of Physiology-Lung Cellular and Molecular Physiology. 1996 Nov 1;271(5):L838-43.","DOI":"10.1152\/ajplung.1996.271.5.L838"},{"key":"ref31","unstructured":"31. Sanbrook J, Fritsch EF, Maniatis T. Molecular cloning: a laboratory manual. Cold Spring Harbor, NY, Cold Spring Harbor Laboratory. 1989;11:31."},{"key":"ref32","doi-asserted-by":"crossref","unstructured":"32. Morgan BR, Artiss JD, Zak B. Calcium determination in serum with stable alkaline Arsenazo III and triglyceride clearing. Clinical chemistry. 1993 Aug 1;39(8):1608-12.","DOI":"10.1093\/clinchem\/39.8.1608"},{"key":"ref33","doi-asserted-by":"crossref","unstructured":"33. Cistola DP, Hamilton JA, Jackson D, Small DM. Ionization and phase behavior of fatty acids in water: application of the Gibbs phase rule. Biochemistry. 1988 Mar 1;27(6):1881-8.","DOI":"10.1021\/bi00406a013"},{"key":"ref34","doi-asserted-by":"crossref","unstructured":"34. Edwards K, Silvander M, Karlsson G. Aggregate structure in dilute aqueous dispersions of oleic acid\/sodium oleate and oleic acid\/sodium oleate\/egg phosphatidylcholine. Langmuir. 1995 Jul;11(7):2429-34.","DOI":"10.1021\/la00007a020"},{"key":"ref35","doi-asserted-by":"crossref","unstructured":"35. Tolin S, De Franceschi G, Spolaore B, Frare E, Canton M, Polverino de Laureto P, Fontana A. The oleic acid complexes of proteolytic fragments of \u03b1-lactalbumin display apoptotic activity. The FEBS journal. 2010 Jan;277(1):163-73.","DOI":"10.1111\/j.1742-4658.2009.07466.x"},{"key":"ref36","doi-asserted-by":"crossref","unstructured":"36. Chirita CN, Necula M, Kuret J. Anionic micelles and vesicles induce tau fibrillization in vitro. Journal of Biological Chemistry. 2003 Jul 11;278(28):25644-50.","DOI":"10.1074\/jbc.M301663200"},{"key":"ref37","doi-asserted-by":"crossref","unstructured":"37. L\u00fccke C, Gantz DL, Klimtchuk E, Hamilton JA. Interactions between fatty acids and \u03b1-synuclein. Journal of lipid research. 2006 Aug 1;47(8):1714-24.","DOI":"10.1194\/jlr.M600003-JLR200"},{"key":"ref38","doi-asserted-by":"crossref","unstructured":"38. Altznauer F, Martinelli S, Yousefi S, Th\u00fcrig C, Schmid I, Conway EM, Sch\u00f6ni MH, Vogt P, Mueller C, Fey MF, Zangemeister-Wittke U. Inflammation-associated cell cycle\u2013independent block of apoptosis by survivin in terminally differentiated neutrophils. Journal of Experimental Medicine. 2004 May 17;199(10):1343-54.","DOI":"10.1084\/jem.20032033"},{"key":"ref39","doi-asserted-by":"crossref","unstructured":"39. Wang T, Li L, Hong W. SNARE proteins in membrane trafficking. Traffic. 2017 Dec;18(12):767-75.","DOI":"10.1111\/tra.12524"},{"key":"ref40","doi-asserted-by":"crossref","unstructured":"40. Okada H, Mak TW. Pathways of apoptotic and non-apoptotic death in tumour cells. Nature Reviews Cancer. 2004 Aug;4(8):592.","DOI":"10.1038\/nrc1412"},{"key":"ref41","doi-asserted-by":"crossref","unstructured":"41. Speliotes EK, Uren A, Vaux D, Horvitz HR. The survivin-like C. elegans BIR-1 protein acts with the Aurora-like kinase AIR-2 to affect chromosomes and the spindle midzone. Molecular cell. 2000 Aug 1;6(2):211-23.","DOI":"10.1016\/S1097-2765(00)00023-X"}],"container-title":["Journal of Medicine and Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/medandlife.org\/wp-content\/uploads\/5.-jml-2021-0065.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,12,31]],"date-time":"2021-12-31T09:08:24Z","timestamp":1640941704000},"score":1,"resource":{"primary":{"URL":"https:\/\/medandlife.org\/wp-content\/uploads\/5.-jml-2021-0065.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":41,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["JMedLife-14-620"],"URL":"https:\/\/doi.org\/10.25122\/jml-2021-0065","relation":{},"ISSN":["1844-122X","1844-3117"],"issn-type":[{"type":"print","value":"1844-122X"},{"type":"electronic","value":"1844-3117"}],"subject":[],"published":{"date-parts":[[2021,1]]}}}