{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T03:02:05Z","timestamp":1792551725001,"version":"4.5.0"},"reference-count":30,"publisher":"S.C. JURNALUL PENTRU MEDICINA SI VIATA S.R.L","issue":"4","license":[{"start":{"date-parts":[[2021,8,1]],"date-time":"2021-08-01T00:00:00Z","timestamp":1627776000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMedLife"],"abstract":"<jats:p>Vitamin D is known to alter immune regulation. It binds to the vitamin D receptors (VDR) expressed on T lymphocytes and macrophages. In individuals with Hashimoto\u2019s thyroiditis, serum vitamin D levels were found to be lower compared to healthy controls. The study\u2019s objective was to investigate the association between VDR gene polymorphism (rs2228570) with blood serum levels of 25-OH vitamin D in patients with thyroid pathology from western Ukraine. The study involved a total of 153 patients with various forms of thyroid pathology. 25-OH vitamin D levels in the serum of the patients and healthy individuals were quantified with ELISA using the 25-OH vitamin D Total (Vit D-Direct) Test System ELISA Kit (Monobind Inc.\u00ae, United States, Product Code: 9425-300) on the EIA Reader Sirio S (Seac, Italy). Genotyping of the VDR (rs2228570) gene polymorphism was performed using TaqMan probes and TaqMan Genotyping Master Mix (4371355) on CFX96\u2122Real-Time PCR Detection System (Bio-Rad Laboratories, Inc., USA). Polymerase chain reaction (PCR) for TaqMan genotyping was carried out according to the kit instructions (Applied Biosystems, USA). Our research identified that that genotype variants of VDR rs2228570 are not risk factors for reduced serum 25-OH vitamin D or vitamin D deficiency in patients with various forms of thyroid pathology patients in the West-Ukrainian population. Vitamin D levels were significantly lower in the carriers of AA and AG genotypes with hypothyroidism caused by autoimmune thyroiditis. In AA genotype carriers with postoperative hypothyroidism, 25-OH vitamin D levels were significantly lower compared to AA genotype carriers with autoimmune thyroiditis.<\/jats:p>","DOI":"10.25122\/jml-2021-0101","type":"journal-article","created":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T05:01:19Z","timestamp":1634274079000},"page":"549-556","source":"Crossref","is-referenced-by-count":15,"title":["25-OH Vitamin D blood serum linkage with VDR gene polymorphism (rs2228570) in thyroid pathology patients in the West-Ukrainian population"],"prefix":"10.25122","volume":"14","author":[{"given":"Iryna Ivanivna","family":"Kamyshna","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Medical Rehabilitation, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Iryna Ivanivna Kamyshna, MD, PhD, Assistant Professor, Department of Medical Rehabilitation, I. Horbachevsky Ternopil National Medical University, Majdan Voli 1, Ternopil, Ukraine, 46001. Phone: +380954313068; E-mail: Iryna.bilous2017@gmail.com","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larysa Borysivna","family":"Pavlovych","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Clinical Immunology, Allergology and Endocrinology, HSEEU Bukovinian State Medical University, Chernivtsi, Ukraine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor Volodymyrovych","family":"Malyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of the System Analysis and Insurance and Financial Mathematics, Yuriy Fedkovych Chernivtsi National University, Chernivtsi, Ukraine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandr Mychailovich","family":"Kamyshnyi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Microbiology, Virology, and Immunology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10755","published-online":{"date-parts":[[2021,8]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Lee H. J., Li C. W., Hammerstad S. S., Stefan M. & Tomer Y. Immunogenetics of autoimmune thyroid diseases: A comprehensive review. J. Autoimmun. 64, 82\u201390, doi: 10.1016\/j.jaut.2015.07.009 (2015).","DOI":"10.1016\/j.jaut.2015.07.009"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Hasham A. & Tomer Y. Genetic and epigenetic mechanisms in thyroid autoimmunity. Immunol. Res. 54, 204\u2013213, doi: 10.1007\/s12026-012-8302-x (2012).","DOI":"10.1007\/s12026-012-8302-x"},{"key":"ref3","unstructured":"Ramesh B. G. et al. Genomics and phenomics of Hashimoto\u2019s thyroiditis in children and adolescents: a prospective study from Southern India. 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