{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T23:24:17Z","timestamp":1780961057677,"version":"3.54.1"},"reference-count":0,"publisher":"S.C. JURNALUL PENTRU MEDICINA SI VIATA S.R.L","issue":"9","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMedLife"],"abstract":"<jats:p><jats:italic>Pseudomonas aeruginosa<\/jats:italic> pathogen is opportunistic. Several virulence factors and biofilms can cause its pathogenicity. Furthermore, infections triggered via multidrug-resistant <jats:italic>P. aeruginosa<\/jats:italic> among hospitalized patients are a public health concern. The primary antimicrobial agents in treating Gram-negative infection include Meropenem and Imipenem. Moreover, the spread of Carbapenem-resistant <jats:italic>P. aeruginosa<\/jats:italic> is a focal concern worldwide. The present research aims to determine the spread of Carbapenem-resistant <jats:italic>P. aeruginosa<\/jats:italic>, and the distribution of the Alginate and Metallo-beta-lactamase encoding gene in clinical isolates. In the present cross-sectional descriptive research, 50 wound and sputum clinical specimens were obtained. Isolates were all identified by applying cultural characteristics and biochemical tests. The Polymerase Chain Reaction (PCR) was conducted to distinguish <jats:italic>algD, BLA-VIM, BLA-IMP<\/jats:italic>, and 16SrRNA genes. Moreover, the phenotypic method was used to detect hemolysin. The disk diffusion technique was applied to screen clinical isolates for eight antimicrobial agents. The PCR results showed all isolates to be positive for <jats:italic>algD<\/jats:italic> and negative for <jats:italic>BLA-VIM<\/jats:italic> and <jats:italic>BLA-IMP<\/jats:italic> genes. Hemolysin and multidrug resistance prevalence was 100% and 76%, respectively. Furthermore, Meropenem proved to be the most efficient antibiotic against clinical isolates. Alginate and hemolysin are considered significant virulence factors for <jats:italic>P. aeruginosa<\/jats:italic>, playing a key role in triggering diseases and tissue or skin lesions. The emergence of Multidrug Resistant (MDR) isolates indicates that developing antibiotic stewardship in our regional community hospital is a top priority. Infection control measures could help control the distribution of virulence genes in <jats:italic>P. aeruginosa<\/jats:italic> isolates. Moreover, regular observation is needed to decrease public health threats, distributing virulence factors and Imipenem-resistance patterns in clinical isolates of <jats:italic>P. aeruginosa<\/jats:italic>.<\/jats:p>","DOI":"10.25122\/jml-2021-0196","type":"journal-article","created":{"date-parts":[[2022,11,20]],"date-time":"2022-11-20T05:33:24Z","timestamp":1668922404000},"page":"1105-1109","source":"Crossref","is-referenced-by-count":3,"title":["Molecular detection of Metallo-Beta-Lactamase and alginate in multidrug resistance Pseudomonas aeruginosa isolated from the clinical specimen"],"prefix":"10.25122","volume":"15","author":[{"given":"Govend Musa","family":"Qader","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Biology, College of Science, University of Salahaddin-Erbil, Kurdistan Region, Iraq","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khanzad Khudhur","family":"Jarjees","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Food Technology, College of Agricultural Engineering Sciences, University of Salahaddin-Erbil, Kurdistan Region, Iraq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Khanzad Khudhur Jarjees, Department of Food Technology, College of Agriculture, University of Salahaddin, Erbil, Iraq. E-mail: khanzad.jarjees@su.edu.krd","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rozhhalat Khudhur","family":"Jarjees","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Pharmacy, Erbil Medical Technical Institute, Erbil Polytechnic University, Erbil, Kurdistan Region, Iraq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10755","published-online":{"date-parts":[[2022,9]]},"container-title":["Journal of Medicine and Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/medandlife.org\/wp-content\/uploads\/JMedLife-15-1105.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,20]],"date-time":"2022-11-20T05:33:24Z","timestamp":1668922404000},"score":1,"resource":{"primary":{"URL":"https:\/\/medandlife.org\/wp-content\/uploads\/JMedLife-15-1105.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["JMedLife-15-1105"],"URL":"https:\/\/doi.org\/10.25122\/jml-2021-0196","relation":{},"ISSN":["1844-122X","1844-3117"],"issn-type":[{"value":"1844-122X","type":"print"},{"value":"1844-3117","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,9]]}}}