{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T10:43:36Z","timestamp":1742381016623},"reference-count":0,"publisher":"S.C. JURNALUL PENTRU MEDICINA SI VIATA S.R.L","issue":"9","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMedLife"],"abstract":"<jats:p>Carbonic anhydrase 9 (CA9) is a marker for decreased O<jats:sub>2<\/jats:sub> concentration and acidosis, associated with poor prognosis in cancerous patients. The current study suggested that the changes in CA9 gene expression level might be used as a predictive marker to assess early prognosis at the time of detection of <jats:italic>de novo<\/jats:italic> leukemia, and then monitor tumor progress during treatment. This study highlights the level of CA9 gene expression in leukemic patients. A total of 44 cases (acute myeloid leukaemia (AML) group: 23 cases; acute lymphoid leukaemia (ALL) group: 13 cases; control group: 8 healthy volunteers) were selected for this study. The CA9 gene expression was assessed by a real-time PCR with the SYBR green assay. A high level of CA9 gene expression was noticed in AML patients compared to the control group, while the results were not significant in ALL patients. After treatment follow-up, significant differences were observed in CA9 gene expression between a complete response and no response in AML patients. As a result, the CA9 tumor gene could act as a potential early marker for acute leukemia prognosis. A low level of CA9 expression was associated with better clinical outcomes, while a high level was related to a negative prognosis in patients with AML.<\/jats:p>","DOI":"10.25122\/jml-2021-0212","type":"journal-article","created":{"date-parts":[[2022,11,20]],"date-time":"2022-11-20T05:34:18Z","timestamp":1668922458000},"page":"1158-1163","source":"Crossref","is-referenced-by-count":4,"title":["Gene expression of carbonic anhydrase 9 (CA9) in de novo acute leukemia as a predictive marker for prognosis"],"prefix":"10.25122","volume":"15","author":[{"given":"Kifah Jabbar","family":"Alyaqubi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Middle Euphrates of Cancer Research Unit, Faculty of Medicine, University of Kufa, Kufa, Iraq","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rasha Hatem","family":"Dosh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Human Anatomy, Faculty of Medicine, University of Kufa, Kufa, Iraq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rawaa Behlul","family":"Al-Fatlawi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Clinical Laboratory Sciences, Faculty of Pharmacy, University of Kufa, Kufa, Iraq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siham Mahmood","family":"Al-Rehemi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Human Anatomy, Faculty of Medicine, University of Kufa, Kufa, Iraq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Najah Rayish","family":"Hadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Kufa, Kufa, Iraq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Najah Rayish Hadi, Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Kufa, Kufa, Iraq. E-mail: drnajahhadi@yahoo.com","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10755","published-online":{"date-parts":[[2022,9]]},"container-title":["Journal of Medicine and Life"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/medandlife.org\/wp-content\/uploads\/JMedLife-15-1158.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,20]],"date-time":"2022-11-20T05:34:19Z","timestamp":1668922459000},"score":1,"resource":{"primary":{"URL":"https:\/\/medandlife.org\/wp-content\/uploads\/JMedLife-15-1158.pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["JMedLife-15-1158"],"URL":"https:\/\/doi.org\/10.25122\/jml-2021-0212","relation":{},"ISSN":["1844-122X","1844-3117"],"issn-type":[{"type":"print","value":"1844-122X"},{"type":"electronic","value":"1844-3117"}],"subject":[],"published":{"date-parts":[[2022,9]]}}}