{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:55:21Z","timestamp":1787025321662,"version":"build-2736575974"},"reference-count":22,"publisher":"S.C. JURNALUL PENTRU MEDICINA SI VIATA S.R.L","issue":"2","license":[{"start":{"date-parts":[[2023,2,1]],"date-time":"2023-02-01T00:00:00Z","timestamp":1675209600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JMedLife"],"abstract":"<jats:p>This study aimed to investigate if C21 could prevent acute renal injury induced by sepsis by regulating the expression of p-AKT\/PI3K. Five equal groups of 25 adult male Swiss-albino mice were randomly divided (n=5): sham (laparotomy without CLP), CLP, vehicle (equivalent amount of DMSO one hour before CLP), and C21 (0.03 mg\/kg, one hour before CLP). ELISA was used to measure serum inflammatory mediators, and the expression of PI3K and P-AKT was determined using PCR and immunohistochemistry (IHC), respectively. TNF, TNF receptor, F8-isoprostane, urea, creatinine, and IL-6 blood levels were considerably lower in the CLP group (p&lt;0.05) compared to the sham group, whereas the C21 treated group had significantly (p&lt;0.05) greater levels of these inflammatory mediators. The IHC analysis revealed that P-AKT expression was significantly lower (p&lt;0.05) in the CLP group compared to the sham group, while the C21 pretreatment group had significantly higher levels of P-AKT expression compared to the CLP group (p&lt;0.05). The PI3K expression in the CLP group was significantly lower than in the sham group (p&lt;0.05), according to PCR results, whereas the PI3K expression in the C21 pretreatment group was significantly greater than in the CLP group (p&lt;0.05). This study showed that C21 might reduce levels of pro-inflammatory cytokines, including TNF-, IL-6, and TNF receptor, by modulating the PI3K\/AKT signaling pathways, which can, in turn, reduce renal dysfunction during CLP-induced sepsis in male mice.<\/jats:p>","DOI":"10.25122\/jml-2022-0299","type":"journal-article","created":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T02:10:10Z","timestamp":1683511810000},"page":"203-209","source":"Crossref","is-referenced-by-count":9,"title":["Investigating the renoprotective effect of C21 in male mice with sepsis via modulation of p-AKT\/PI3K expression"],"prefix":"10.25122","volume":"16","author":[{"given":"Huda","family":"Jabber","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Pharmacology and Therapeutics, College of Medicine, University of Al-Qadisiyah, Iraq","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bassim","family":"Mohammed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Pharmacology and Therapeutics, College of Medicine, University of Al-Qadisiyah, Iraq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najah Rayish","family":"Hadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Kufa, Kufa, Iraq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"* Corresponding Author: Najah Rayish Hadi, Department of Pharmacology & Therapeutics, Faculty of Medicine, University of Kufa, Kufa, Iraq. E-mail: drnajahhadi@yahoo.com","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10755","published-online":{"date-parts":[[2023,2]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"1. Singer M, Deutschman CS, Seymour CW, Shankar-Hari M,\n                      et al.\n                      The Third International Consensus Definitions for Sepsis and Septic Shock (Sepsis-3). JAMA. 2016 Feb 23;315(8):801-10. doi: 10.1001\/jama.2016.0287.","DOI":"10.1001\/jama.2016.0287"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"2. Rudd KE, Johnson SC, Agesa KM, Shackelford KA,\n                      et al.\n                      Global, regional, and national sepsis incidence and mortality, 1990-2017: analysis for the Global Burden of Disease Study. 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