{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T06:16:30Z","timestamp":1792995390060,"version":"4.5.0"},"reference-count":22,"publisher":"Scientific Scholar","content-domain":{"domain":["cytojournal.com"],"crossmark-restriction":true},"short-container-title":["Cytojournal"],"abstract":"\n Objective:<\/jats:title>\n The function of tumor protein p53-inducible nuclear protein 2 (TP53INP2) in numerous cancers has been elucidated, but its role across the development of spinal cord glioma (SCG) remains largely unexplored. This study aims to explore the anti-cancer effect of TP53INP2\/nuclear factor-kappa B (NF-kB)\/nuclear factor erythroid 2-related factor 2 (Nrf2)\/heme oxygenase-1 (HO-1) pathway in SCG.<\/jats:p>\n <\/jats:sec>\n \n Material and Methods:<\/jats:title>\n \n Quantitative real-time polymerase chain reaction was employed to determine TP53INP2 messenger RNA expression\n in vitro<\/jats:italic>\n . Western blot analysis was conducted to detect TP53INP2, epithelial-tomesenchymal transition (EMT) marker, and NF-kB\/Nrf2\/HO-1 pathway protein. The proliferative potentials of glioma cells were assessed by 5-ethynyl-2\u2019-deoxyuridine, colony formation, and cell counting kit-8 assays. Transwell assays were used to evaluate migratory and invasive capacities. Apoptotic cells and reactive oxygen species were analyzed using flow cytometer. Enzyme-linked immunosorbent assay was performed to measure superoxide dismutase and glutathione peroxidase levels. A tumor xenograft model in mouse was established.\n <\/jats:p>\n <\/jats:sec>\n \n Results:<\/jats:title>\n High expression of TP53INP2 was observed in glioma cells. TP53INP2 depletion significantly inhibited tumor growth, metastasis, EMT, and oxidative stress and increased the apoptosis rate and number of immune cells. The silenced TP53INP2 hampered the activation of NF-kB and promoted the activation of the Nrf2\/HO-1 pathway.<\/jats:p>\n <\/jats:sec>\n \n Conclusion:<\/jats:title>\n This work highlights the therapeutic potential of TP53INP2\/NF-kB\/Nrf2\/HO-1 axis in SCG.<\/jats:p>\n <\/jats:sec>","DOI":"10.25259\/cytojournal_13_2025","type":"journal-article","created":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T13:03:11Z","timestamp":1761224591000},"page":"87","update-policy":"https:\/\/doi.org\/10.25259\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The anti-cancer role of tumor protein p53-inducible nuclear protein 2\/nuclear factor-kB\/nuclear factor erythroid 2-related factor 2\/heme oxygenase-1 pathway in spinal cord glioma"],"prefix":"10.25259","volume":"22","author":[{"given":"Jiang","family":"Huang","sequence":"first","affiliation":[{"name":"Orthopedic Hospital, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhimin","family":"Pan","sequence":"additional","affiliation":[{"name":"Orthopedic Hospital, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Jiangxi, China"},{"name":"Department of Orthopedics, The Fourth Medical Center, Chinese PLA General Hospital, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yu","sequence":"additional","affiliation":[{"name":"Orthopedic Hospital, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Zhu","sequence":"additional","affiliation":[{"name":"Orthopedic Hospital, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangbin","family":"Wang","sequence":"additional","affiliation":[{"name":"Orthopedic Hospital, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanguo","family":"Zhou","sequence":"additional","affiliation":[{"name":"Orthopedic Hospital, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, The Second Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, China,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2025,10,23]]},"reference":[{"key":"10.25259\/Cytojournal_13_2025\/ref-1","doi-asserted-by":"publisher","first-page":"13","DOI":"10.14245\/ns.2143210.605","article-title":"Glioma immunotherapy: Advances and challenges for spinal cord gliomas","volume":"19","author":"Grady","year":"2022","journal-title":"Neurospine"},{"key":"10.25259\/Cytojournal_13_2025\/ref-2","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1186\/s40478-021-01222-6","article-title":"Molecular diagnostics helps to identify distinct subgroups of spinal astrocytomas","volume":"9","author":"Biczok","year":"2021","journal-title":"Acta Neuropathol Commun"},{"key":"10.25259\/Cytojournal_13_2025\/ref-3","doi-asserted-by":"publisher","first-page":"436","DOI":"10.1053\/j.sult.2023.03.012","article-title":"The role of MRI in the diagnosis of spinal cord tumors","volume":"44","author":"De Paiva","year":"2023","journal-title":"Semin Ultrasound CT MR"},{"key":"10.25259\/Cytojournal_13_2025\/ref-4","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1007\/s00066-018-1366-3","article-title":"Treatment outcomes of radiotherapy for primary spinal cord glioma","volume":"195","author":"Choi","year":"2019","journal-title":"Strahlenther Onkol"},{"key":"10.25259\/Cytojournal_13_2025\/ref-5","doi-asserted-by":"publisher","first-page":"1341","DOI":"10.1080\/15548627.2020.1713646","article-title":"The bifunctional role of TP53INP2 in transcription and autophagy","volume":"16","author":"Xu","year":"2020","journal-title":"Autophagy"},{"key":"10.25259\/Cytojournal_13_2025\/ref-6","doi-asserted-by":"publisher","first-page":"e34034","DOI":"10.1371\/journal.pone.0034034","article-title":"DOR\/Tp53inp2 and Tp53inp1 constitute a metazoan gene family encoding dual regulators of autophagy and transcription","volume":"7","author":"Sancho","year":"2012","journal-title":"PLoS One"},{"key":"10.25259\/Cytojournal_13_2025\/ref-7","doi-asserted-by":"publisher","first-page":"870","DOI":"10.1091\/mbc.e08-07-0671","article-title":"The TP53INP2 protein is required for autophagy in mammalian cells","volume":"20","author":"Nowak","year":"2009","journal-title":"Mol Biol Cell"},{"key":"10.25259\/Cytojournal_13_2025\/ref-8","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1016\/j.molcel.2014.12.013","article-title":"Deacetylation of nuclear LC3 drives autophagy initiation under starvation","volume":"57","author":"Huang","year":"2015","journal-title":"Mol Cell"},{"key":"10.25259\/Cytojournal_13_2025\/ref-9","doi-asserted-by":"publisher","first-page":"9587","DOI":"10.2147\/OTT.S251830","article-title":"TP53INP2 modulates epithelial-to-mesenchymal transition via the GSK-3beta\/beta-catenin\/snail1 pathway in bladder cancer cells","volume":"13","author":"Zhou","year":"2020","journal-title":"Onco Targets Ther"},{"key":"10.25259\/Cytojournal_13_2025\/ref-10","doi-asserted-by":"publisher","first-page":"630794","DOI":"10.3389\/fgene.2021.630794","article-title":"Integrative analysis of TP53INP2 in head and neck squamous cell carcinoma","volume":"12","author":"Cao","year":"2021","journal-title":"Front Genet"},{"key":"10.25259\/Cytojournal_13_2025\/ref-11","doi-asserted-by":"publisher","first-page":"149275","DOI":"10.1016\/j.bbrc.2023.149275","article-title":"TP53INP2 modulates the malignant progression of colorectal cancer by reducing the inactive form of b-catenin","volume":"690","author":"Shi","year":"2024","journal-title":"Biochem Biophys Res Commun"},{"key":"10.25259\/Cytojournal_13_2025\/ref-12","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1387\/ijdb.210180gk","article-title":"Epithelial-to-mesenchymal transition of tumor cells: Cancer progression and metastasis","volume":"66","author":"Vardas","year":"2022","journal-title":"Int J Dev Biol"},{"key":"10.25259\/Cytojournal_13_2025\/ref-13","doi-asserted-by":"publisher","first-page":"118782","DOI":"10.1016\/j.bbamcr.2020.118782","article-title":"Epithelial-to-mesenchymal transition as the driver of changing carcinoma and glioblastoma microenvironment","volume":"1867","author":"Majc","year":"2020","journal-title":"Biochim Biophys Acta Mol Cell Res"},{"key":"10.25259\/Cytojournal_13_2025\/ref-14","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1016\/j.ccell.2020.06.001","article-title":"Oxidative stress in cancer","volume":"38","author":"Hayes","year":"2020","journal-title":"Cancer Cell"},{"key":"10.25259\/Cytojournal_13_2025\/ref-15","doi-asserted-by":"publisher","first-page":"112133","DOI":"10.1016\/j.fct.2021.112133","article-title":"Roflumilast protects from cisplatin-induced testicular toxicity in male rats and enhances its cytotoxicity in prostate cancer cell line, Role of NF-kB-p65, cAMP\/PKA and Nrf2\/HO-1, NQO1 signaling","volume":"151","author":"Abdel-Wahab","year":"2021","journal-title":"Food Chem Toxicol"},{"key":"10.25259\/Cytojournal_13_2025\/ref-16","doi-asserted-by":"publisher","first-page":"1541","DOI":"10.1007\/s11010-020-04005-0","article-title":"Modulatory effect of Tagetes erecta flowers essential oils via Nrf2\/HO-1\/NF-kB\/p65 axis mediated suppression of N-methyl-N'nitro-Nnitroguanidine (MNNG) induced gastric cancer in rats","volume":"476","author":"Cui","year":"2021","journal-title":"Mol Cell Biochem"},{"key":"10.25259\/Cytojournal_13_2025\/ref-17","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1097\/COC.0000000000000434","article-title":"Literature review of spinal cord glioblastoma","volume":"41","author":"Timmons","year":"2018","journal-title":"Am J Clin Oncol"},{"key":"10.25259\/Cytojournal_13_2025\/ref-18","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/j.cmet.2020.06.002","article-title":"Targeting DGAT1 ameliorates glioblastoma by increasing fat catabolism and oxidative stress","volume":"32","author":"Cheng","year":"2020","journal-title":"Cell Metab"},{"key":"10.25259\/Cytojournal_13_2025\/ref-19","doi-asserted-by":"publisher","first-page":"620677","DOI":"10.3389\/fmolb.2020.620677","article-title":"Drug resistance in glioblastoma: The two faces of oxidative stress","volume":"7","author":"Olivier","year":"2020","journal-title":"Front Mol Biosci"},{"key":"10.25259\/Cytojournal_13_2025\/ref-20","doi-asserted-by":"publisher","first-page":"295","DOI":"10.7150\/ijbs.10586","article-title":"Hydroferrate fluid, MRN-100, provides protection against chemical-induced gastric and esophageal cancer in Wistar rats","volume":"11","author":"Ghoneum","year":"2015","journal-title":"Int J Biol Sci"},{"key":"10.25259\/Cytojournal_13_2025\/ref-21","doi-asserted-by":"publisher","first-page":"1120","DOI":"10.1016\/j.ijbiomac.2017.12.109","article-title":"Structural characterization of a polysaccharide from Sargassum henslowianum, and its immunomodulatory effect on gastric cancer rat","volume":"108","author":"Han","year":"2018","journal-title":"Int J Biol Macromol"},{"key":"10.25259\/Cytojournal_13_2025\/ref-22","doi-asserted-by":"publisher","first-page":"29740","DOI":"10.1038\/s41598-024-80856-7","article-title":"Triptolide induces apoptosis of glioma cells by inhibiting NF-kB activation during oxidative stress","volume":"14","author":"Li","year":"2024","journal-title":"Sci Rep"}],"container-title":["Cytojournal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cytojournal.com\/the-anti-cancer-role-of-tumor-protein-p53-inducible-nuclear-protein-2-nuclear-factor-kb-nuclear-factor-erythroid-2-related-factor-2-heme-oxygenase-1-pathway-in-spinal-cord-glioma\/","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cytojournal.com\/the-anti-cancer-role-of-tumor-protein-p53-inducible-nuclear-protein-2-nuclear-factor-kb-nuclear-factor-erythroid-2-related-factor-2-heme-oxygenase-1-pathway-in-spinal-cord-glioma\/","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T13:03:11Z","timestamp":1761224591000},"score":1,"resource":{"primary":{"URL":"https:\/\/cytojournal.com\/the-anti-cancer-role-of-tumor-protein-p53-inducible-nuclear-protein-2-nuclear-factor-kb-nuclear-factor-erythroid-2-related-factor-2-heme-oxygenase-1-pathway-in-spinal-cord-glioma\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,23]]},"references-count":22,"alternative-id":["10.25259\/Cytojournal_13_2025"],"URL":"https:\/\/doi.org\/10.25259\/cytojournal_13_2025","relation":{},"ISSN":["1742-6413","0974-5963"],"issn-type":[{"value":"1742-6413","type":"electronic"},{"value":"0974-5963","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10,23]]},"article-number":"87"}}