{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:22:46Z","timestamp":1791595366085,"version":"4.3.4"},"reference-count":31,"publisher":"Scientific Scholar","content-domain":{"domain":["cytojournal.com"],"crossmark-restriction":true},"short-container-title":["Cytojournal"],"abstract":"<jats:sec id=\"st1\">\n<jats:title>Objective:<\/jats:title>\n<jats:p>This study aimed to explore the clinical significance of CD45<jats:sup>+<\/jats:sup>CD33<jats:sup>+<\/jats:sup>CD14<jats:sup>\u2212<\/jats:sup>CD10<jats:sup>\u2212<\/jats:sup>HLA-DR<jats:sup>\u2212\/low<\/jats:sup> neutrophils (Cluster of Differentiation 10 [CD10<jats:sup>\u2212<\/jats:sup>] neutrophils) in B-cell non-Hodgkin\u2019s lymphoma (B-NHL). An amplification system of CD10<jats:sup>\u2212<\/jats:sup> neutrophils <jats:italic>in vitro<\/jats:italic> was constructed using cytokines, and the mechanisms underlying the cytokine-induced expansion and activation of the CD10<jats:sup>\u2212<\/jats:sup> neutrophil subpopulation were investigated.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"st2\">\n<jats:title>Material and Methods:<\/jats:title>\n<jats:p>We identified a novel suppressive cell population known as CD10<jats:sup>\u2212<\/jats:sup> neutrophils in the peripheral blood of patients with B-NHL in different statuses by flow cytometry and found it to be correlated with interleukin-6 levels, T cell counts, and plasma arginase-1 (Arg-1) levels. We then verified the effect of CD10<jats:sup>\u2212<\/jats:sup> neutrophil expression on the prognosis of patients with B-NHL. Furthermore, we described a clinically compatible method for generating granulocyte populations rich in CD10<jats:sup>\u2212<\/jats:sup> neutrophils using cultures of peripheral blood-isolated neutrophils supplemented with cytokines <jats:italic>in vitro<\/jats:italic>. Arg-1 expression was detected in neutrophils before and after induction by cytokines through reverse-transcription polymerase chain reaction, enzyme-linked immunosorbent assay, and flow cytometry. T-cell proliferation and apoptosis were measured by carboxyfluorescein succinimidyl ester assay and Annexin V-Propidium Iodide stains, and induced cells were exposed to Arg-1 inhibitor and ruxolitinib. signal transducer and activator of transcription 3 (STAT3)\/Arg-1 signaling was studied mainly by western blot and chromatin immunoprecipitation experiments.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"st3\">\n<jats:title>Results:<\/jats:title>\n<jats:p>We established a correlation between high CD10<jats:sup>\u2212<\/jats:sup> neutrophil levels and poorer survival outcomes in patients with B-NHL. Moreover, CD10<jats:sup>\u2212<\/jats:sup> neutrophils were positively correlated with interleukin (IL)-6, T-reg cells, and plasma Arg-1 levels and negatively correlated with the absolute number of total T cells. Granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor, and IL-6 could all induce the expansion of CD10<jats:sup>\u2212<\/jats:sup> neutrophil phenotype cells <jats:italic>in vitro<\/jats:italic>, which exhibit typical immature cellular morphology, and the combination of IL-6 and GM-CSF was the most effective. We confirmed that the STAT3\/Arg-1 signaling pathway could be a critical mechanism regulating CD10<jats:sup>\u2212<\/jats:sup> neutrophil-mediated immunosuppression <jats:italic>in vitro<\/jats:italic>.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"st4\">\n<jats:title>Conclusion:<\/jats:title>\n<jats:p>CD10<jats:sup>\u2212<\/jats:sup> neutrophils exhibited basic characteristics similar to conventional myeloid-derived suppressor cells. Our observations provide a promising STAT3 or Arg-1 targeting strategy for B-NHL and an important method for generating remarkably amounts of inhibitory granulocyte populations rich in CD10<jats:sup>\u2212<\/jats:sup> neutrophils for immunotherapy.<\/jats:p>\n<\/jats:sec>","DOI":"10.25259\/cytojournal_165_2024","type":"journal-article","created":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T11:52:12Z","timestamp":1734695532000},"page":"69","update-policy":"https:\/\/doi.org\/10.25259\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Association of immunosuppressive CD45<sup>+<\/sup>CD33<sup>+<\/sup>CD14<sup>\u2212<\/sup> CD10<sup>\u2212<\/sup>HLA-DR<sup>\u2212\/low<\/sup> neutrophils with poor prognosis in patients with lymphoma and their expansion and activation through STAT3\/arginase-1 pathway <i>in vitro<\/i>"],"prefix":"10.25259","volume":"21","author":[{"given":"Meng","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Hematology, Hematological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui., China,"},{"name":"Department of Hematology, Jining NO.1 People\u2019s Hospital, Jining, Shandong, China,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Health Statistics, School of Public Health, Anhui Medical University, Hefei, Anhui, China,"},{"name":"School of Nursing, Anhui Medical University, Hefei, Anhui, China,"},{"name":"Nursing International Collaboration Research Center of Anhui Province, Hefei, Anhui, China,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanqiu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hematology, Hematological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui., China,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Hematology, Hematological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui., China,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinjing","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Hematology, Hematological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui., China,"},{"name":"Department of Laboratory, Fuyang People\u2019s Hospital, Fuyang, China,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Hematology, Hematological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui., China,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinli","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Hematology, Hematological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui., China,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xunyi","family":"Jiao","sequence":"additional","affiliation":[{"name":"Department of Hematology, Hematological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui., China,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhimin","family":"Zhai","sequence":"additional","affiliation":[{"name":"Department of Hematology, Hematological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui., China,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiping","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematology, Hematological Research Center, The Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui., China,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2024,12,20]]},"reference":[{"key":"10.25259\/Cytojournal_165_2024\/ref-1","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1038\/s41392-021-00670-9","article-title":"Myeloid-derived suppressor cells as immunosuppressive regulators and therapeutic targets in cancer","volume":"6","author":"Li","year":"2021","journal-title":"Signal Transduct Target Ther"},{"key":"10.25259\/Cytojournal_165_2024\/ref-2","doi-asserted-by":"publisher","first-page":"12150","DOI":"10.1038\/ncomms12150","article-title":"Recommendations for myeloid-derived suppressor cell nomenclature and characterization standards","volume":"7","author":"Bronte","year":"2016","journal-title":"Nat Commun"},{"key":"10.25259\/Cytojournal_165_2024\/ref-3","doi-asserted-by":"publisher","first-page":"875","DOI":"10.1016\/j.immuni.2021.04.004","article-title":"MDSC: Markers, development, states, and unaddressed complexity","volume":"54","author":"Hegde","year":"2021","journal-title":"Immunity"},{"key":"10.25259\/Cytojournal_165_2024\/ref-4","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1189\/jlb.4RI0515-204R","article-title":"Transcriptional regulation of myeloid-derived suppressor cells","volume":"98","author":"Condamine","year":"2015","journal-title":"J Leukoc Biol"},{"key":"10.25259\/Cytojournal_165_2024\/ref-5","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1186\/s12943-022-01657-y","article-title":"Myeloid-derived suppressor cells: An emerging target for anticancer immunotherapy","volume":"21","author":"Wu","year":"2022","journal-title":"Mol Cancer"},{"key":"10.25259\/Cytojournal_165_2024\/ref-6","doi-asserted-by":"publisher","first-page":"1791","DOI":"10.1016\/S0140-6736(23)02705-8","article-title":"B-cell non-Hodgkin lymphomas","volume":"403","author":"Silkenstedt","year":"2024","journal-title":"Lancet"},{"key":"10.25259\/Cytojournal_165_2024\/ref-7","doi-asserted-by":"publisher","first-page":"e168","DOI":"10.1097\/HS9.0000000000000168","article-title":"Myeloid-derived suppressor cells in hematologic diseases: Promising biomarkers and treatment targets","volume":"3","author":"Bizymi","year":"2019","journal-title":"Hemasphere"},{"key":"10.25259\/Cytojournal_165_2024\/ref-8","first-page":"363","article-title":"The research progress and clinical application of human CD10 molecule","volume":"37","author":"Liu","year":"2014","journal-title":"Int J Immunol"},{"key":"10.25259\/Cytojournal_165_2024\/ref-9","doi-asserted-by":"publisher","first-page":"449","DOI":"10.1189\/jlb.5MA1116-453RR","article-title":"Expansion and activation of granulocytic, myeloid-derived suppressor cells in childhood precursor B cell acute lymphoblastic leukemia","volume":"102","author":"Liu","year":"2017","journal-title":"J Leukoc Biol"},{"key":"10.25259\/Cytojournal_165_2024\/ref-10","doi-asserted-by":"publisher","first-page":"1343","DOI":"10.1182\/blood-2016-04-713206","article-title":"Mature CD10+and immature CD10-neutrophils present in G-CSF-treated donors display opposite effects on T cells","volume":"129","author":"Marini","year":"2017","journal-title":"Blood"},{"key":"10.25259\/Cytojournal_165_2024\/ref-11","doi-asserted-by":"publisher","first-page":"256","DOI":"10.1111\/imcb.12728","article-title":"CD14-CD10-CD45+HLA-DR-SSC+ neutrophils may be granulocytic myeloid-derived suppressor cell-like cells and relate to disease progression in non-Hodgkin's lymphoma patients","volume":"102","author":"Zhou","year":"2024","journal-title":"Immunol Cell Biol"},{"key":"10.25259\/Cytojournal_165_2024\/ref-12","first-page":"707","article-title":"Chinese Association for Clinical Oncologists, Medical Oncology Branch of Chinese International Exchange and Promotion Association for Medical and Healthcare. Clinical practice guideline for lympoma in China (2021 Edition)","volume":"43","author":"China Anti-cancer Association Lymphoma Committee","year":"2021","journal-title":"Zhonghua Zhong Liu Za Zhi"},{"key":"10.25259\/Cytojournal_165_2024\/ref-13","doi-asserted-by":"publisher","first-page":"2191","DOI":"10.1001\/jama.2013.281053","article-title":"World Medical Association Declaration of Helsinki: Ethical principles for medical research involving human subjects","volume":"310","author":"World Medical Association","year":"2013","journal-title":"JAMA"},{"key":"10.25259\/Cytojournal_165_2024\/ref-14","doi-asserted-by":"publisher","first-page":"2159","DOI":"10.1182\/blood-2018-11-844548","article-title":"Neutrophil plasticity in the tumor microenvironment","volume":"133","author":"Giese","year":"2019","journal-title":"Blood"},{"key":"10.25259\/Cytojournal_165_2024\/ref-15","doi-asserted-by":"publisher","first-page":"471","DOI":"10.3389\/fimmu.2015.00471","article-title":"How neutrophils shape adaptive immune responses","volume":"6","author":"Leliefeld","year":"2015","journal-title":"Front Immunol"},{"key":"10.25259\/Cytojournal_165_2024\/ref-16","doi-asserted-by":"publisher","first-page":"938","DOI":"10.3389\/fimmu.2020.00938","article-title":"Myeloid cell-derived arginase in cancer immune response","volume":"11","author":"Grzywa","year":"2020","journal-title":"Front Immunol"},{"key":"10.25259\/Cytojournal_165_2024\/ref-17","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1186\/s12943-020-01258-7","article-title":"Targeting STAT3 in cancer immunotherapy","volume":"19","author":"Zou","year":"2020","journal-title":"Mol Cancer"},{"key":"10.25259\/Cytojournal_165_2024\/ref-18","doi-asserted-by":"publisher","first-page":"105","DOI":"10.31138\/mjr.31.1.105","article-title":"Therapeutic targeting of JAKs: from hematology to rheumatology and from the first to the second generation of JAK inhibitors","volume":"31","author":"Bertsias","year":"2020","journal-title":"Mediterr J Rheumatol"},{"key":"10.25259\/Cytojournal_165_2024\/ref-19","doi-asserted-by":"publisher","first-page":"1580","DOI":"10.1172\/JCI60083","article-title":"STAT3 regulates arginase-I in myeloid-derived suppressor cells from cancer patients","volume":"123","author":"Vasquez-Dunddel","year":"2013","journal-title":"J Clin Invest"},{"key":"10.25259\/Cytojournal_165_2024\/ref-20","doi-asserted-by":"publisher","first-page":"7363084","DOI":"10.1155\/2020\/7363084","article-title":"Circulating monocytic myeloid-derived suppressor cells are elevated and associated with poor prognosis in acute myeloid leukemia","volume":"2020","author":"Wang","year":"2020","journal-title":"J Immunol Res"},{"key":"10.25259\/Cytojournal_165_2024\/ref-21","doi-asserted-by":"publisher","first-page":"104","DOI":"10.3389\/fonc.2015.00104","article-title":"Clinical impact of the immunome in lymphoid malignancies: The role of myeloid-derived suppressor cells","volume":"5","author":"Vetro","year":"2015","journal-title":"Front Oncol"},{"key":"10.25259\/Cytojournal_165_2024\/ref-22","doi-asserted-by":"publisher","first-page":"e12954","DOI":"10.1371\/journal.pone.0012954","article-title":"The density of CD10 corresponds to commitment and progression in the human B lymphoid lineage","volume":"5","author":"Ichii","year":"2010","journal-title":"PLoS One"},{"key":"10.25259\/Cytojournal_165_2024\/ref-23","doi-asserted-by":"publisher","first-page":"2439","DOI":"10.1084\/jem.20100587","article-title":"HIF-1aregulates function and differentiation of myeloid-derived suppressor cells in the tumor microenvironment","volume":"207","author":"Corzo","year":"2010","journal-title":"J Exp Med"},{"key":"10.25259\/Cytojournal_165_2024\/ref-24","doi-asserted-by":"publisher","first-page":"561","DOI":"10.3390\/cells9030561","article-title":"Myeloid-derived suppressor cells as a therapeutic target for cancer","volume":"9","author":"Law","year":"2020","journal-title":"Cells"},{"key":"10.25259\/Cytojournal_165_2024\/ref-25","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1080\/10428194.2020.1817431","article-title":"Increased myeloid-derived suppressor cells in patients with myelodysplastic syndromes suppress CD8+T lymphocyte function through the STAT3-ARG1 pathway","volume":"62","author":"Qi","year":"2021","journal-title":"Leuk Lymphoma"},{"key":"10.25259\/Cytojournal_165_2024\/ref-26","doi-asserted-by":"publisher","first-page":"4717","DOI":"10.3390\/jcm10204717","article-title":"Increased monocytic myeloid-derived suppressor cells in whole blood predict poor prognosis in patients with plasma cell myeloma","volume":"10","author":"Bae","year":"2021","journal-title":"J Clin Med"},{"key":"10.25259\/Cytojournal_165_2024\/ref-27","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1016\/j.jid.2018.07.006","article-title":"CXCL5 as Regulator of neutrophil function in cutaneous melanoma","volume":"139","author":"Forsthuber","year":"2019","journal-title":"J Invest Dermatol"},{"key":"10.25259\/Cytojournal_165_2024\/ref-28","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1016\/j.it.2019.01.006","article-title":"Neutrophil degranulation, plasticity, and cancer metastasis","volume":"40","author":"Mollinedo","year":"2019","journal-title":"Trends Immunol"},{"key":"10.25259\/Cytojournal_165_2024\/ref-29","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/j.imbio.2016.02.001","article-title":"The diversity of circulating neutrophils in cancer","volume":"222","author":"Mishalian","year":"2017","journal-title":"Immunobiology"},{"key":"10.25259\/Cytojournal_165_2024\/ref-30","doi-asserted-by":"publisher","first-page":"2254","DOI":"10.1182\/blood-2010-12-325753","article-title":"A human promyelocytic-like population is responsible for the immune suppression mediated by myeloid-derived suppressor cells","volume":"118","author":"Solito","year":"2011","journal-title":"Blood"},{"key":"10.25259\/Cytojournal_165_2024\/ref-31","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1038\/s41590-017-0022-x","article-title":"Myeloid-derived suppressor cells coming of age","volume":"19","author":"Veglia","year":"2018","journal-title":"Nat Immunol"}],"container-title":["Cytojournal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cytojournal.com\/association-of-immunosuppressive-cd45cd33cd14-cd10hla-dr-low-neutrophils-with-poor-prognosis-in-patients-with-lymphoma-and-their-expansion-and-activation-through-stat3-argina\/","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cytojournal.com\/association-of-immunosuppressive-cd45cd33cd14-cd10hla-dr-low-neutrophils-with-poor-prognosis-in-patients-with-lymphoma-and-their-expansion-and-activation-through-stat3-argina\/","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,20]],"date-time":"2024-12-20T11:52:14Z","timestamp":1734695534000},"score":1,"resource":{"primary":{"URL":"https:\/\/cytojournal.com\/association-of-immunosuppressive-cd45cd33cd14-cd10hla-dr-low-neutrophils-with-poor-prognosis-in-patients-with-lymphoma-and-their-expansion-and-activation-through-stat3-argina\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,20]]},"references-count":31,"alternative-id":["10.25259\/Cytojournal_165_2024"],"URL":"https:\/\/doi.org\/10.25259\/cytojournal_165_2024","relation":{},"ISSN":["1742-6413","0974-5963"],"issn-type":[{"value":"1742-6413","type":"electronic"},{"value":"0974-5963","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12,20]]},"article-number":"69"}}