{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T14:06:28Z","timestamp":1776866788061,"version":"3.51.2"},"reference-count":35,"publisher":"Scientific Scholar","content-domain":{"domain":["cytojournal.com"],"crossmark-restriction":true},"short-container-title":["Cytojournal"],"abstract":"<jats:sec id=\"st1\">\n<jats:title>Objective<\/jats:title>\n<jats:p>Liposarcomas are rare tumors, and it is difficult to collect cases in less densely populated areas. Therefore, we aimed to document more cases over a relatively long period to provide more data about the characteristics of liposarcomas. In this study, the clinicopathological features of liposarcomas were investigated in 27 patients.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"st2\">\n<jats:title>Material and Methods<\/jats:title>\n<jats:p>All cases were confirmed by diagnosis through hematoxylin and eosin staining, immunohistochemistry (IHC), and fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH). Combined IHC analysis was performed for murine double minute 2 (MDM2), cyclin-dependent kinase 4 (CDK4), multiple tumor suppressor 1 (P16), and Cyclin D1. FISH was performed to detect MDM2 amplification in atypical lipomatous tumor\/well-differentiated liposarcoma (ALT\/WDLPS) and dedifferentiated liposarcoma (DDLPS), and DNA damage inducible transcript 3 ( DDIT3) rearrangements in myxoid liposarcoma (MLPS).<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"st3\">\n<jats:title>Results<\/jats:title>\n<jats:p>Seven cases of liposarcoma were located in the paratesticular region (25.9%, 7\/27), 12 in the retroperitoneum (44.4%, 12\/27), and eight in the limbs (29.6%, 8\/27). Histological analysis showed that there were 13 cases of ALT\/WDLPS (48.1%, 13\/27), nine cases of DDLPS (33.3%, 9\/27), three cases of MLPS (11.1%, 3\/27), and two cases of pleomorphic liposarcoma (7.4%, 2\/27). IHC analysis revealed that 26 cases were MDM2-positive (96.3%, 26\/27), 22 were CDK4-positive (81.5%, 22\/27), 26 were P16-positive (96.3%, 26\/27), and 27 were cyclin D1-positive (100%, 27\/27). FISH analysis revealed 20 cases of MDM2 positivity (90.9%, 20\/22) and one case of DDIT3 positivity (50%, 1\/2). The clinical outcomes were available for 21 patients. Four patients died (4\/21, 19.0%), five experienced recurrence (5\/21, 23.8%), and 12 (12\/21, 57.1%) survived with no other disease.<\/jats:p>\n<\/jats:sec>\n<jats:sec id=\"st4\">\n<jats:title>Conclusion<\/jats:title>\n<jats:p>A combined IHC examination of the four indicators may be used to diagnose ALT\/WDLPS and DDLPS, and FISH is recommended as an important supporting method.<\/jats:p>\n<\/jats:sec>","DOI":"10.25259\/cytojournal_246_2024","type":"journal-article","created":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T05:30:15Z","timestamp":1743485415000},"page":"40","update-policy":"https:\/\/doi.org\/10.25259\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Clinicopathological and molecular diagnostic features of liposarcoma: A study of 27 cases"],"prefix":"10.25259","volume":"22","author":[{"given":"Yin","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Pathology, Ningbo Yinzhou No.2 Hospital, Ningbo, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Ningbo Yinzhou No.2 Hospital, Ningbo, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Ningbo Yinzhou No.2 Hospital, Ningbo, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuo","family":"Wang","sequence":"additional","affiliation":[{"name":"Hangzhou Cancer Institution, Hangzhou Cancer Hospital, Hangzhou, China,"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","published-online":{"date-parts":[[2025,4,1]]},"reference":[{"key":"10.25259\/Cytojournal_246_2024\/ref-1","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1111\/his.12311","article-title":"Liposarcomas: Diagnostic pitfalls and new insights","volume":"64","author":"Dei","year":"2014","journal-title":"Histopathology"},{"key":"10.25259\/Cytojournal_246_2024\/ref-2","doi-asserted-by":"publisher","first-page":"644","DOI":"10.1111\/his.14265","article-title":"Updates from the 2020 World Health Organization classification of soft tissue and bone tumours","volume":"78","author":"Anderson","year":"2021","journal-title":"Histopathology"},{"key":"10.25259\/Cytojournal_246_2024\/ref-3","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1177\/10668969221099628","article-title":"Well differentiated liposarcoma presenting as a duodenal polyp: A case report and review of the literature","volume":"31","author":"Warnick","year":"2023","journal-title":"Int J Surg Pathol"},{"key":"10.25259\/Cytojournal_246_2024\/ref-4","doi-asserted-by":"publisher","first-page":"3230","DOI":"10.3390\/jcm10153230","article-title":"Biology and management of dedifferentiated liposarcoma: State of the art and perspectives","volume":"10","author":"Nishio","year":"2021","journal-title":"J Clin Med"},{"key":"10.25259\/Cytojournal_246_2024\/ref-5","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1097\/PAS.0000000000002037","article-title":"Does \u201cLow-Grade\u201d dedifferentiated liposarcoma exist? 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