{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T06:01:43Z","timestamp":1790402503838,"version":"4.1.0"},"reference-count":33,"publisher":"Scientific Scholar","content-domain":{"domain":["cytojournal.com"],"crossmark-restriction":true},"short-container-title":["Cytojournal"],"abstract":"<jats:sec id=\"st1\">\n                    <jats:title>Objectives:<\/jats:title>\n                    <jats:p>This study investigated the potential of folate receptor-positive circulating tumor cells (FR+CTCs) as a non-invasive biomarker for breast cancer diagnosis and clinical stratification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"st2\">\n                    <jats:title>Materials and Methods:<\/jats:title>\n                    <jats:p>Between January 2024 and October 2024, 179 breast cancer patients and 38 patients with benign breast diseases were enrolled at Sichuan Provincial People\u2019s Hospital, China. FR+CTCs were isolated from peripheral blood using the CytoploRare\u00ae kit and quantified via real-time polymerase chain reaction. Diagnostic accuracy was assessed using receiver operating characteristic (ROC) analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"st3\">\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      FR+CTC levels were significantly higher in breast cancer patients compared to those with benign diseases (\n                      <jats:italic>P<\/jats:italic>\n                      &lt;0.001). The ROC analysis yielded an area under the curve of 0.871 (95% confidence interval: 0.818\u2013 0.924), with a sensitivity of 82.12% and specificity of 84.21%. In addition, FR+CTC levels correlated significantly with T, N, M, and tumor node metastasis stages, as well as molecular subtypes, particularly human epidermal growth factor receptor-2 (\n                      <jats:italic>P<\/jats:italic>\n                      &lt;0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"st4\">\n                    <jats:title>Conclusion:<\/jats:title>\n                    <jats:p>FR+CTCs demonstrate significant potential as a non-invasive biomarker for breast cancer, showing high diagnostic accuracy and associations with key clinical characteristics. While promising, these findings warrant validation in larger, more diverse populations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.25259\/cytojournal_250_2025","type":"journal-article","created":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T05:36:11Z","timestamp":1790400971000},"page":"58","update-policy":"https:\/\/doi.org\/10.25259\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Folate receptor-positive circulating tumor cells as a potential biomarker for breast cancer diagnosis"],"prefix":"10.25259","volume":"23","author":[{"given":"Juan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Breast Surgery, Sichuan Provincial People\u2019s Hospital, University of Electronic Science and Technology of China, Chengdu, China,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery, Sichuan Provincial People\u2019s 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