{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,8]],"date-time":"2026-11-08T07:34:41Z","timestamp":1794123281120,"version":"4.8.0"},"reference-count":11,"publisher":"Scientific Scholar","content-domain":{"domain":["ijmr.org.in"],"crossmark-restriction":true},"short-container-title":["Indian J Med Res"],"abstract":"<jats:sec>\n                    <jats:title>Background and objectives<\/jats:title>\n                    <jats:p>Only 10% of the individuals infected with Mycobacterium tuberculosis (MTB) manifest active tuberculosis. Interferon-\u03b3 release assay (IGRA) and tuberculin skin test using purified protein derivative (PPD) are used to identify tuberculosis (TB) infection. We aimed to study the comparative performance of Cy-Tb (the SIILTIBCY skin test) and PPD with IGRA as the reference standard.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In step-I, 30 TB patients and 32 IGRA-negative health controls; in step-II, 2651 participants aged 1 to 85 yr; and in step- III, 647 household contacts of TB patients aged 1-18 years old were enrolled. Blood for IGRA was drawn immediately prior to intra-dermal administration of Cy-Tb (the SIILTIBCY skin test) or PPD. The size of induration of Cy-Tb \u22655 mm or PPD \u2265 10mm after 48 to 72 h were considered positive. Combined Data from step II and step III were analysed for sensitivity, specificity, agreement, and kappa-coefficient with IGRA as the reference standard using Stata version 14.2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The step-I results for Cy-Tb showed 90% specificity, 93.8% specificity, and an agreement of 91.9% with IGRA and a Cohen\u2019s kappa coefficient of 0.83. Combined analysis of step-II and step-III data revealed sensitivity of 82.6% (95% CI 79.1-85.8), specificity of 71.1% (95% CI 68.2-73.8), agreement of 74.8%, and kappa of 0.5 for Cy-Tb. PPD showed sensitivity and specificity of 71.0% (95% CI 66.7-75.0) and 71.9% (95% CI 69.0-74\u03876), respectively with 71.6% agreement and kappa of 0.4 with IGRA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Interpretation and conclusions<\/jats:title>\n                    <jats:p>Performance of Cy-Tb was comparable to that of PPD with IGRA as reference standard. Cy-Tb skin test can be used for diagnosis of TB infection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.25259\/ijmr_1794_2025","type":"journal-article","created":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T19:55:41Z","timestamp":1775418941000},"page":"309-318","update-policy":"https:\/\/doi.org\/10.25259\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Diagnostic accuracy and safety of Cy-Tb for detection of tuberculosis infection: A multicentric study from India"],"prefix":"10.25259","volume":"163","author":[{"given":"Manjula","family":"Singh","sequence":"first","affiliation":[{"name":"Division of Delivery Research, \u2020Indian Council of Medical Research, New Delhi, India"},{"name":"Academy of Scientific and Innovative Research, Ghaziabad, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahziba","family":"Hussain","sequence":"additional","affiliation":[{"name":"Department of Microbiology, \u2020\u2020ICMR-Regional Medical Research Centre, Bhubaneswar, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bhavesh","family":"Modi","sequence":"additional","affiliation":[{"name":"Department of Community Medicine, GMERS Medical college, Gandhinagar, India"},{"name":"Department of Community Medicine, All India Institute of Medical Sciences, Rajkot, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjeev","family":"Nair","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine, Government Medical College, Trivandrum, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunil","family":"Kumar","sequence":"additional","affiliation":[{"name":"State TB Cell, Kerala, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasir","family":"Alvi","sequence":"additional","affiliation":[{"name":"Department of Community Medicine, Hamdard Institute of Medical Sciences and Research, New Delhi, 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Vinod","family":"Pantula","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Medicine, Bhagwan Mahavir Medical Research Center, Hyderabad, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijaya L.","family":"Valluri","sequence":"additional","affiliation":[{"name":"Department of Immunology, Bhagwan Mahavir Medical Research Center, Hyderabad, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirankumar","family":"Rade","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, WHO India, STOP TB Partnership, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajendra Prasad","family":"Joshi","sequence":"additional","affiliation":[{"name":"Central TB Division, Ministry of Health and Family Welfare, Government of India, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjay 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