{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:25:17Z","timestamp":1790270717779,"version":"4.1.0"},"reference-count":29,"publisher":"Scientific Scholar","content-domain":{"domain":["ijmr.org.in"],"crossmark-restriction":true},"short-container-title":["IJMR"],"abstract":"\n Background & objectives<\/jats:title>\n \n OXA-232 is a five amino acid substitution variant of OXA-48 and is reported in carbapenem-resistant\n Klebsiella pneumoniae<\/jats:italic>\n (CRKP), which is associated with nosocomial infections among immunocompromised patients in the intensive care unit. This study aimed to characterise\n bla<\/jats:italic>\n OXA-232<\/jats:sub>\n in CRKP of clinical origin and investigate its transcriptional response against sub-inhibitory levels of carbapenems.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n CRKP was isolated from blood (pathogens) and stool cultures (colonisers) of neonates and was characterized for\n bla<\/jats:italic>\n OXA-232<\/jats:sub>\n . Co-existing resistance determinants were investigated in\n bla<\/jats:italic>\n OXA-232<\/jats:sub>\n positive isolates, followed by horizontal gene transferability assay and PCR-based replicon typing (PBRT). Cloning of\n bla<\/jats:italic>\n OXA-232<\/jats:sub>\n was performed, and expression of\n bla<\/jats:italic>\n OXA-232<\/jats:sub>\n in the isolates and their clones under sub-inhibitory concentrations of carbapenems was checked\n via<\/jats:italic>\n RT-PCR. Mobile genetic elements associated with\n bla<\/jats:italic>\n OXA-232<\/jats:sub>\n were investigated, followed by DNA fingerprinting through enterobacterial repetitive intergenic consensus (ERIC) PCR.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n bla<\/jats:italic>\n OXA-232<\/jats:sub>\n with co-carriage of extended-spectrum beta-lactamases (ESBLs), sulphonamides and quinolones were identified in seven CRPK isolates recovered from blood samples of neonates. Transformation and cloning of\n bla<\/jats:italic>\n OXA-232<\/jats:sub>\n was successful. The sub-inhibitory concentration of carbapenems induces elevated expression of this resistant determinant. IS\n Ecp1<\/jats:italic>\n was associated with\n bla<\/jats:italic>\n OXA-232<\/jats:sub>\n in the upstream region within two haplotypes of CRKP isolates of clinical origin.\n <\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions<\/jats:title>\n Selective carbapenem pressure resulted in higher expression of this gene, which could account for treatment failure. With frequent reports of occurrence among clinical isolates, monitoring and further investigation of this novel variant are necessary to understand its transmission dynamics and to thwart its further dissemination.<\/jats:p>\n <\/jats:sec>","DOI":"10.25259\/ijmr_1915_22","type":"journal-article","created":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T12:27:53Z","timestamp":1725884873000},"page":"644-652","update-policy":"https:\/\/doi.org\/10.25259\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Characterization of\n bla<\/i>\n OXA-232<\/sub>\n \n \n <\/i>\n carrying carbapenem-resistant\n Klebsiella pneumoniae<\/i>\n (CRKP) & their expression profiles under selective carbapenem pressure: An in-depth study from India"],"prefix":"10.25259","volume":"159","author":[{"given":"Bhaskar Jyoti","family":"Das","sequence":"first","affiliation":[{"name":"Department of Microbiology, Assam University, Silchar, Assam, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuhina","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jayalaxmi","family":"Wangkheimayum","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Assam University, Silchar, Assam, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kajal","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashok","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amitabha","family":"Bhattacharjee","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Assam University, Silchar, Assam, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2024,9,7]]},"reference":[{"key":"10.25259\/IJMR_1915_22\/ref-1","doi-asserted-by":"crossref","first-page":"229","DOI":"10.3389\/fpubh.2019.00229","article-title":"An outbreak of carbapenem-resistant and hypervirulent Klebsiella pneumoniae in an intensive care unit of a major teaching hospital in Wenzhou, China","volume":"7","author":"Zhao","year":"2019","journal-title":"Front Public Health"},{"key":"10.25259\/IJMR_1915_22\/ref-2","doi-asserted-by":"crossref","first-page":"3216","DOI":"10.1016\/j.transproceed.2014.09.159","article-title":"Bloodstream infections caused by carbapenemase-producing Klebsiella pneumoniae among intensive care unit patients after 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