{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T16:36:47Z","timestamp":1781195807005,"version":"3.54.1"},"reference-count":26,"publisher":"Scientific Scholar","content-domain":{"domain":["ijmr.org.in"],"crossmark-restriction":true},"short-container-title":["Indian J Med Res"],"abstract":"<jats:sec>\n<jats:title>Background &amp; objectives<\/jats:title>\n<jats:p>The global impact of COVID-19, with over 45 million cases and 533,300 deaths in India alone, necessitates effective surveillance methods. Traditional approaches face challenges in detecting pre-symptomatic and asymptomatic cases, prompting the exploration of wastewater-based epidemiology (WBE). This study focuses on Mumbai\u2019s vulnerable slums, aiming to assess the potential of WBE as an alternative surveillance method.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Methods<\/jats:title>\n<jats:p>Genomic surveillance of SARS-CoV-2 was conducted in Mumbai\u2019s vulnerable settings (slums) for 11 months (August 2022 to June 2023). Wastewater samples from open drains and sewage treatment plants were correlated with reported COVID-19 cases in the city. Early detection of emerging viral variants and seasonal variations in viral load were explored.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Results<\/jats:title>\n<jats:p>Correlations were identified between wastewater samples and reported COVID-19 cases in Mumbai\u2019s vulnerable slums, with early detection occurring three weeks before clinical diagnoses, underscoring the potential utility of WBE. Genomic sequencing provided insights into the viral variants, identifying shifts in predominant variants. Seasonal variations showed higher viral concentrations in summer and monsoon, potentially associated with accelerated droplet evaporation in early summer and droplet-based transmission during mid-summer and monsoon.<\/jats:p>\n<\/jats:sec>\n<jats:sec>\n<jats:title>Interpretation &amp; conclusions<\/jats:title>\n<jats:p>Wastewater-based epidemiology emerges as a cost-effective and rapid early warning system, providing crucial insights into virus behaviour and evolution. Particularly significant for countries like India, WBE aids in outbreak monitoring and targeted interventions. The global integration of wastewater surveillance emphasizes its importance in comprehensive pandemic monitoring, establishing it as an integral component of public health strategies worldwide.<\/jats:p>\n<\/jats:sec>","DOI":"10.25259\/ijmr_299_24","type":"journal-article","created":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T05:44:19Z","timestamp":1736487859000},"page":"570-577","update-policy":"https:\/\/doi.org\/10.25259\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Wastewater-based genomic surveillance of SARS-CoV-2 in vulnerable communities in Mumbai"],"prefix":"10.25259","volume":"160","author":[{"given":"Pratibha Prakash","family":"Kadam","sequence":"first","affiliation":[{"name":"Department of Tuberculosis, The Foundation for Medical Research, Mumbai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tejal","family":"Mestry","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, The Foundation for 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